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Wall-breaking method for haematococcus pluvialis

A technology of Haematococcus pluvialis and algae, applied in the direction of microorganism-based methods, biochemical equipment and methods, and the dissolution of microorganisms, which can solve problems such as the complexity of the extraction process

Inactive Publication Date: 2015-07-08
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jiang Ling and others [5] Ultrasonic crushing is used for grinding, and the extraction rate is high, but it needs to be combined with acid heating, which makes the extraction process complicated

Method used

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  • Wall-breaking method for haematococcus pluvialis
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  • Wall-breaking method for haematococcus pluvialis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Plate culture and storage of algae: 1. Add 15% agar powder to BBM (Bold's Basal Medium) medium, sterilize at 121°C for 20 minutes, cool to 50°C, and pour it into a plate with a thickness of about 0.5cm; 2. Use Pick a small amount of algae from the old plate with the inoculation loop and paint on the new plate; 3. Place the plate at a temperature of 23.8°C and a light intensity of 20 μmol photos m -2 the s -1 4. Finally, store the plates at room temperature and away from light, repaint every two plates, and store them after subculture to maintain the activity of algae species. The composition of BBM plate medium is NaNO 3 2.9mM, MgSO 4 0.3mM, NaCl 0.43mM, K 2 HPO 4 ·3H 2 O 0.43mM, KH 2 PO 4 1.29mM, CaCl 2 2H 2 O 0.17mM, KOH 0.55mM, H 3 BO 3 0.18mM, EDTA-Na 2 0.12mM, ZnSO 4 ·7H 2 O 0.0307mM, MnCl 2 4H 2 O 0.0073mM, sodium glacial molybdate 0.0049mM, CuSO 4 ·5H 2 O 0.0063mM, Co(NO 3 ) 2 ·6H 2 O 0.0017mM, EDTA-Fe 3+ 1.8mM, agar 15-20g / L, pH 6.8, ste...

Embodiment 2

[0047] 1) Plate culture and preservation of algae: 1. Add 15% agar powder to BBM (Bold's Basal Medium) medium, sterilize at 121°C for 20 minutes, cool to 50°C, and pour it into a plate with a thickness of about 0.5cm; 2. Use Pick a small amount of algae from the old plate with the inoculation loop and paint on the new plate; 3. Place the plate at a temperature of 23.8°C and a light intensity of 20 μmol photos m -2 the s -1 4. Finally, store the plates at room temperature and away from light, repaint every two plates, and store them after subculture to maintain the activity of algae species. The composition of BBM plate medium is NaNO 3 2.9mM, MgSO 4 0.3mM, NaCl 0.43mM, K 2 HPO 4 ·3H 2 O 0.43mM, KH 2 PO 4 1.29mM, CaCl 2 2H 2 O 0.17mM, KOH 0.55mM, H 3 BO 3 0.18mM, EDTA-Na 2 0.12mM, ZnSO 4 ·7H 2 O 0.0307mM, MnCl 2 4H 2 O 0.0073mM, sodium glacial molybdate 0.0049mM, CuSO 4 ·5H 2 O 0.0063mM, Co(NO 3 ) 2 ·6H 2 O 0.0017mM, EDTA-Fe 3+ 1.8mM, agar 15-20g / L, pH 6.8...

Embodiment 3

[0057] 1) Plate culture and preservation of algae: 1. Add 15% agar powder to BBM (Bold's Basal Medium) medium, sterilize at 121°C for 20 minutes, cool to 50°C, and pour it into a plate with a thickness of about 0.5cm; 2. Use Pick a small amount of algae from the old plate with the inoculation loop and paint on the new plate; 3. Place the plate at a temperature of 23.8°C and a light intensity of 20 μmol photos m -2 the s -1 4. Finally, store the plates at room temperature and away from light, repaint the plates every 2 times, and store them after subculture to maintain the activity of algae species. The composition of BBM plate medium is NaNO 3 2.9mM, MgSO 4 0.3mM, NaCl 0.43mM, K 2 HPO 4 ·3H 2 O 0.43mM, KH 2 PO 4 1.29mM, CaCl 2 2H 2 O 0.17mM, KOH 0.55mM, H 3 BO 3 0.18mM, EDTA-Na 2 0.12mM, ZnSO4 ·7H 2 O 0.0307mM, MnCl 2 4H 2 O 0.0073mM, sodium glacial molybdate 0.0049mM, CuSO 4 ·5H 2 O 0.0063mM, Co(NO 3 ) 2 ·6H 2 O 0.0017mM, EDTA-Fe 3+ 1.8mM, agar 15-20g / L, ...

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Abstract

The invention provides a wall-breaking method for haematococcus pluvialis, and relates to a breaking method for a freshwater algae cell wall. Algal species plate cultivation is performed; counting is performed on algal liquid through a blood counting chamber, the algal liquid is inoculated into a triangular bottle containing a first-class seed BBM+V culture medium to be cultured, and first-class seed liquid is obtained when algal bodies grow to a logarithm growth period; the first-class seed liquid is taken to be inoculated into a triangular bottle containing a second-class seed BBM+V culture medium to be cultured, the triangular bottle is shaken by hands for twice everyday to prevent the algal bodies from adhering to the bottle wall, and second-class seed liquid is obtained; a fermentation medium is contained in a triangular bottle, the second-class seed liquid is inoculated according to the initial algal cell density of 5*104 cells / ml, the triangular bottle is placed in an illuminating incubator to be cultured and shaken by hands for twice regularly everyday, and three biological repetitions are performed on each set of cells; wall-breaking treatment is performed on the algal bodies by utilizing a high-pressure homogenizing breaking method or a high-speed bead milling method or a manual grinding method, a sample is taken to be used for analysis on parameters such as carotenoid releasing amount and cell breaking rate.

Description

technical field [0001] The invention relates to a method for crushing the cell wall of freshwater algae, in particular to a cell wall crushing method for obtaining the maximum sporulation rate and astaxanthin yield from Haematococcus pluvialis H. Background technique [0002] Astaxanthin is a ketone carotenoid that widely exists in nature. It has a good coloring function and is widely distributed in aquatic animals such as salmon, trout, crustaceans, krill, etc., but the aquatic animals themselves Astaxanthin cannot be synthesized, so astaxanthin has important application value in aquatic feed additives. In addition, astaxanthin is known as "super vitamin E", and its antioxidant performance is 500 times that of vitamin E. The strong antioxidant and free radical scavenging ability of astaxanthin makes it play an important role in the health of the human body, and can effectively prevent tissues, cells and DNA from being oxidatively damaged [1] . Super strong antioxidant ca...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12R1/89
CPCC12N1/066
Inventor 江志军卢英华凌雪萍谢友坪潘雪珊
Owner XIAMEN UNIV
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