Method for preparing high-purity all-trans lycopene crystal

A technology of lycopene and all-trans, which is applied in the field of chemical pharmaceuticals, can solve the problems of low solubility of lycopene, decrease of the ratio of all-trans, increase of product cost, etc., and achieve the effect of convenient extraction, purification and separation operation, and good dehydration

Active Publication Date: 2009-04-29
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the mycelium contains a large amount of water, the drying process and the subsequent concentration of the extract will cause lycopene to be oxidized, lost or isomerized to reduce the ratio of all-trans, and the solubility of lycopene in ester solvents is small, so the extraction Requires the use of large amounts of solvent, increasing product cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Use the feed liquid fermented by B. trispora to filter the mycelium, take 1 kg (containing 16.0 g of lycopene), put it in a basket grinding mill, add 3 kg of ethanol, and grind for 15 minutes at 5°C , centrifugal filtration. Add 2 kg of n-hexane to the mycelium, stir and impregnate at 10°C for half an hour, filter and deoil. Add 20 kg of n-hexane to the mycelium, stir and extract at 35°C for 15 minutes, filter, and slowly cool the filtrate to -5°C while stirring, stir and crystallize for 12 hours, filter, and dry the crystallization in vacuum at 35°C to obtain lycopene 9.8 grams, the extraction yield is 61.3%.

Embodiment 2

[0029] Take 3 kg of mycelium of Brassella trispora (containing 48.0 g of lycopene), put it in a basket grinder, add 15 kg of ethanol, grind for 30 minutes at 20° C., and centrifuge. Add 15 kg of n-hexane to the mycelium, stir and impregnate at 0°C for half an hour, filter and deoil. 150 kg of mycelium n-hexane, stirred and extracted at 45°C for 15 minutes, filtered, and the filtrate was slowly cooled to -5°C under stirring, stirred and crystallized for 12 hours, filtered, and vacuum-dried at 35°C to obtain 33.4 grams of lycopene , and the extraction yield was 69.6%.

Embodiment 3

[0031] Take 3 kg of mycelium of Brassella trispora (containing 48.0 g of lycopene), put it in a basket mill, add 30 kg of ethanol, grind for 30 minutes at 5°C, and centrifuge. Add 9 kg of n-hexane to the mycelia, stir and impregnate at 5°C for half an hour, filter and deoil. Add 50 kg of n-hexane to the mycelium, stir and extract at 55°C for 10 minutes, filter, and then stir and extract the filter residue twice in the same way, combine the filtrate, slowly cool down to -5°C under stirring, stir and crystallize for 12 hours, filter, and crystallize Vacuum drying at 35°C yielded 40.4 g of lycopene with a yield of 84.2%.

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Abstract

The invention discloses a preparation method of high-purity all-trans lycopene crystal, and comprises the following steps: filtering the ferment material liquid, thus obtaining mycelium; adding polar organic solvent to the mycelium to grind and break the wall, and then filtering and removing the organic solvent; taking the mixture of mycelium and low-boiling petroleum based organic solvent as degreaser to remove mycelium oily consistituent and the foreign matter such as little amount of Beta-carotene; taking the mixture of mycelium and low-boiling petroleum based organic solvent as extractingagent to liquid-solid extract the lycopene in mycelium; cooling down the extract liquor for crystallization; filtering and drying in vacuum, thus obtaining high-purity all-trans lycopene crystal. Thetechnique adopted by the method can avoid lycopene from retrogradation and isomerization owing to heat and oxidation. The lycopene crystal prepared by the method has high purity, the content of lycopene exceeds over 97 percent, and the content of the all-trans structure exceeds over 90 percent.

Description

technical field [0001] The invention relates to a preparation method of high-purity all-trans lycopene crystal, which belongs to the field of chemical pharmacy. Background technique [0002] Lycopene is a natural pigment, a polyene compound containing 40 carbons, a straight-chain hydrocarbon composed of 11 conjugated and 2 non-conjugated carbon-carbon double bonds, and there are all-trans and many cis Common cis isomers such as 5-cis, 9-cis, 13-cis, 15-cis, all-trans and cis lycopene have different chemical properties and bioavailability in the human body. Similarly, it is generally believed that all-trans has greater physiological activity, because naturally occurring lycopene is mainly all-trans. Due to the structural characteristics of lycopene, it is easy to undergo oxidative degradation and isomerization into a cis structure during processing and storage, making it difficult to obtain high-purity all-trans lycopene. [0003] Currently, there are three methods for prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C11/21C07C7/10C07C7/14C12P5/02C12R1/01
CPCY02E50/343Y02E50/30
Inventor 侯卫南王健王志伟李建州肖江峰杜尔凤陈晓丹
Owner JIANGSU ALAND NOURISHMENT
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