Method for improving dewaterability of activated sludge through addition of microbial flocculant
A microbial flocculant and activated sludge technology, which is applied in the direction of dehydration/drying/thickened sludge treatment, can solve the problems of poor dehydration performance and high cost of mud cake subsequent treatment and disposal, and achieves easy operation, low investment and operation cost, and source wide range of effects
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[0043] Example 1
[0044] The fermentation broth after shaking culture was centrifuged at 5000r / min for 30min, and the supernatant was the liquid microbial flocculant. The isolated and screened Klebsiella flocculant was used to treat 4g / L kaolin suspension solution. 0.4g kaolin, 5mL CaCl passed through a 60 mesh sieve 2 (1%) The solution and 2mL fermentation supernatant are mixed in a 250mL beaker, add deionized water to 100mL, a magnetic stirrer 400r / min fast stirring for 1min, 150r / min slow stirring for 5min, transfer to a 100mL graduated cylinder and let stand for 5min. Measure the absorbance (A) of the supernatant liquid at a wavelength of 550nm with an ultraviolet spectrophotometer, select a fresh medium instead of the fermentation broth, and measure the absorbance of the treated kaolin suspension as a control (A) 0 ) The calculation formula of flocculation rate (Fr) is as follows:
[0045] Fr = A 0 - A A 0 X 100 % - - - ( 1 )
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[0048] Example 2
[0049] Klebsiella flocculant is applied to conditioning sludge to improve the dewatering performance of sludge. Add 0~5mL microbial flocculant to 25mL sample of sludge to be tested, stir at 400r / min for 60s and slowly at 150r / min for 5min in a magnetic stirrer, then move to the sludge specific resistance measuring device (attached) image 3 ), filter under the condition of a constant vacuum of 0.05MPa, record the filtration time t and the corresponding filtrate volume V. The sludge specific resistance (SRF) and moisture content were selected to characterize the sludge dewatering effect, and the moisture content was measured by gravimetric method. The specific resistance in filtration (SRF) represents the resistance per unit mass of sludge per unit filtration area when filtered under constant pressure. The larger the sludge specific resistance, the worse the filtration performance. The calculation of the sludge specific resistance is shown in the formula (2):
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