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Process method for producing yeast polysaccharide and yeast nucleotide from yeast

A technology of zymosan and a process method, which is applied in the field of yeast production of zymosan and nucleotides, can solve the problems of inability to realize large-scale industrial application, high procurement cost of hydrolyzed protease, inability to realize production cost control, etc., and achieve process production cost control. , The effect of breaking the wall is significant, and the time of enzymatic hydrolysis and alkali treatment is shortened

Inactive Publication Date: 2011-09-07
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

This method uses the cavitation effect of ultrasonic waves to achieve the effect of breaking the wall. Due to the complicated operation of the ultrasonic breaker, the processing capacity is small (the processing capacity per hour is about several liters to more than ten liters), and it is easy to generate high temperature (the local instantaneous high temperature can reach 10000000000000000000000000 5000°C, the temperature of the product after treatment is also about 70°C, high temperature will denature the protein, and the active ingredient will lose its activity), the process conditions are high, and it is generally only used in laboratories and other places with small processing capacity
At present, this method is only in the experimental stage, and cannot realize large-scale industrial application.
And because the cell wall of yeast is thicker than ordinary cells, up to 0.1-0.3 μm, while the thickness of general bacterial cell walls is only about tens of nanometers, in order to ensure the efficiency of enzymatic hydrolysis, the amount of protease added is usually controlled at 200-210U / g dry matter , and the purchase cost of hydrolytic protease is high, if the usage of protease cannot be reduced, the purpose of substantive production cost control cannot be achieved

Method used

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  • Process method for producing yeast polysaccharide and yeast nucleotide from yeast
  • Process method for producing yeast polysaccharide and yeast nucleotide from yeast

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Take yeast powder (waste beer yeast) and add water to the yeast suspension with a concentration of 10% (m / m), homogenize the yeast suspension at 60Mpa high pressure for 3 times, then centrifuge at 3000r / min for 5min, collect the precipitate, press 120U / g dry matter was added with papain and neutral protease (the mass ratio of the two was 1:1), the pH was 5, 55°C constant temperature enzymatic hydrolysis for 6 hours, and then centrifuged at 3000r / min for 5min to obtain the enzymatic hydrolysis precipitate and enzymatic hydrolysis supernatant, , add 3% NaOH (m / m) solution to the enzymolysis precipitate with 2ml / g dry matter, treat at 90°C for 2 hours, centrifuge at 3000r / min for 5min, collect the alkali-treated precipitate, wash it with water several times until neutral, and spray dry The zymosan was obtained (detected by the phenol sulfuric acid method, the product polysaccharide had a purity of 85.4%, and the yield was 10.5%); the enzymolysis supernatant was spray-dried ...

Embodiment 2

[0024] Take yeast powder (beer waste yeast) and add water to a yeast suspension with a concentration of 12%. Homogenize the yeast suspension at 55Mpa high pressure for 3 times, then centrifuge at 3500r / min for 5min, collect the sediment, and press 130U / g dry matter Add papain and neutral protease (the mass ratio of the two is 1:1), enzymolyze at 60°C for 7 hours, then centrifuge at 3000r / min for 5min to obtain the enzymolysis precipitate and enzymolysis supernatant, add 2ml to the enzymolysis precipitate Add 3% NaOH solution per g of dry matter, treat at 95°C for 2 hours, centrifuge at 3000r / min for 5min, collect the alkali-treated precipitate, wash it with water for several times until neutral, and spray dry to obtain zymosan (detected by the phenol-sulfuric acid method, the purity of the product polysaccharide reached 80.2%, and the yield was 8.3%); the enzymatic hydrolysis supernatant was inactivated by instant high-temperature enzyme and then spray-dried to obtain yeast nuc...

Embodiment 3

[0026] Take yeast powder (beer waste yeast) and add water to a yeast suspension with a concentration of 11%, homogenize the yeast suspension twice at 50Mpa high pressure, then centrifuge at 3000r / min for 5min, collect the sediment, and press 110U / g dry matter Add papain, enzymolysis at constant temperature at 50°C for 5h, then centrifuge at 3000r / min for 5min to obtain the enzymolysis precipitate and enzymolysis supernatant, add 3% NaOH solution to the enzymolysis precipitate at 2ml / g dry matter, and treat at 85°C for 2 hours, 3000r / min centrifuged for 5min, collected the alkali-treated precipitate, washed it with water for several times until neutral, and spray-dried to obtain zymosan (detected by the phenol-sulfuric acid method, the product polysaccharide purity reached 77.3%, and the yield was 8.0%); enzymolysis supernatant Yeast nucleotides (crude protein content 43.2%, RNA content 14.5%) were obtained by spray-drying after instantaneous high-temperature enzyme inactivation...

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Abstract

The invention relates to a process method for producing yeast polysaccharide and yeast nucleotide from yeast, which solves the problems of low yield, long period, high equipment investment and high production cost existing in the conventional process for extracting the yeast polysaccharide and the yeast nucleotide. The yeast polysaccharide and the yeast nucleotide are respectively extracted by taking waste beer yeast as a raw material through the steps of high-pressure homogenization and performing of an enzyme method. The process method has the characteristics of high yield and purity of extracted yeast polysaccharide, high yeast nucleotide content, short extraction time, low enzyme consumption, low equipment investment and production cost and suitability for large-scale industrial production.

Description

technical field [0001] The invention relates to a yeast treatment process, in particular to a process method for yeast to produce zymosan and nucleotides. Background technique [0002] The waste yeast in the production of beer industry in our country is mostly treated cheaply as roughage raw material, resulting in a lot of waste of resources. If the waste yeast is fully developed and utilized so that the effective components in it can be recovered reasonably, the economic benefits will be considerable. At present, the treatment of waste yeast mainly uses waste yeast as raw material to extract zymosan by acid-base method or enzyme-alkaline method. Polysaccharides, but the required enzyme concentration is high, and the time for enzymolysis and alkali treatment is long (enzymolysis time is at least 12h, and alkali treatment time is at least 4h), which greatly increases production costs and affects production efficiency and production scale. Masson et al. published a "Study on...

Claims

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Application Information

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IPC IPC(8): C12N15/10C08B37/00
Inventor 王春维吴灵英朱惠玲王锐徐栋王兆钧刘昌杜
Owner WUHAN POLYTECHNIC UNIVERSITY
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