The invention which relates to a method for preparing an immobilized nitrilase belongs to the technical field of immobilized enzymes. According to the invention, the immobilized nitrilase is prepared by treating a nitrilase sold in the market as a raw material and a lysine-macroporous ion exchange resin sold in the market as a carrier, and carrying out simple processes of immobilization, suction filtration and lavation. The method of the invention has the advantages of simplicity and easy implementation, mild reaction condition, simple and easily available equipment, no discharge of "three wastes", full substance resource utilization, low production cost, and convenient popularization and application; and the immobilized nitrilase has the advantages of small steric hindrance effect, high heat stability, wide use range, and long service life. The method can be widely used for preparing the immobilized enzymes, and products prepared through adopting the method of the invention can be widely applied to the biodegradation industry, the bioremediation industry, the pharmaceutical industry, the chemical engineering industry, the agricultural industry and the like.