Pretreatment liquid applied before analysis of pathogenic bacterium sample and pretreatment method

Abstract

The invention relates to a pretreatment liquid applied before analysis of a pathogenic bacterium sample and a pretreatment method. The pretreatment liquid applied before analysis of the pathogenic bacterium sample is prepared from tris(hydroxymethyl) aminomethane, calcium salt, phospholipase, lysozyme and double distilled water; in terms of every 100 ml of the pretreatment liquid applied before analysis of the pathogenic bacterium sample, the content of tris(hydroxymethyl) aminomethane is 0.0121-0.121 g, the content of the calcium salt is 0.05-0.5 g in terms of the calcium element, the content of the phospholipase is 0.003-0.03 g, and the content of the lysozyme is 0.025-0.1 g; the pH of the pretreatment liquid applied before analysis of the pathogenic bacterium sample is 7.0-8.5. The pretreatment liquid applied before analysis of the pathogenic bacterium sample and the pretreatment method are provided, so that the sensitivity of direct pathogen detection methods such as colloidal gold, ELISA (enzyme linked immunosorbent assay) and the like on the basis of an antigen-antibody capture reaction is improved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a pretreatment liquid and a pretreatment method before analysis of a pathogenic bacteria sample. Background technique [0002] The history of human health and medical development is a history of fighting against various infectious diseases. At present, infectious diseases (including infectious diseases) account for more than 25% of all human deaths. Infectious diseases caused by bacterial infection occupy the first place. In 2014, the World Health Organization released the global cause of death data, which showed that among the top ten causes of death in the world, lower respiratory tract infection, HIV / AIDS, diarrheal disease and tuberculosis ranked 4th, 6th, 7th and 10th, among which lower respiratory tract infection , diarrhea and tuberculosis are mostly bacterial infections. The inspection of pathogenic bacteria is the main means of clinical diagnosis of infectious disea...

AI Technical Summary

Problems solved by technology

However, serological tests can only provide a retrospective diagnosis and sometimes require double sera

In addition, the timing of antibody appearance is not easy to grasp, and there are differences in specific antibodies between children, adolescents and adults, so the detection quality of existing serological methods is also limited

[0006] The third is to use molecular biology techniques to detect the presence of pathogen DNA, the most commonly used of which is polymerase chain reaction (PCR). And the operation requirements are high, and false positives are prone to occur, so it cannot be used as a commonly used clinical diagnosis method in my country

The fact is that sometimes the extracellular domain of the cell surface antigen (usually a protein) can be successfully found, but due to the complexity of the cell surface structure, such as steric hindrance, the antibody prepared therefrom cannot effectively bind to the cell surface antigen. antigen, which reduces the detection sensitivity of the method

Therefore, the types of pathogenic bacteria that can be detected by this method are less, which greatly limits the application of this method.