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Method for producing yeast autolysate by probiotic fermentation

A technology of yeast hydrolyzate and yeast, which is applied in the field of probiotic fermentation to produce yeast hydrolyzate, can solve the problems of large loss of nutrients, influence on enzymatic hydrolysis efficiency, and high requirements for equipment conditions, so as to reduce production costs, have broad application prospects, and break wall effect noticeable effect

Active Publication Date: 2015-02-11
青岛玛斯特生物技术有限公司 +1
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] ①The acid-base hydrolysis method is to add acid or alkali to the yeast cell to break the wall. The commonly used acid and alkali are hydrochloric acid, sulfuric acid, NaOH, etc. This method is cheap and the yield of amino acid is high, but the hydrolyzed The loss of micronutrients in the product is large, the taste is poor, the color is not good, and the environment is polluted. Today, the requirements for food safety are getting higher and the pressure on environmental protection is getting higher and higher. This method is gradually eliminated.
[0005] ②Chemical wall-breaking method: toluene has the best wall-breaking effect, but it is highly toxic, while chloroform, ethyl acetate, paraben, etc. have general wall-breaking effects, and there is a problem of residual chemical toxic substances
[0006] ③Mechanical method: including high-pressure homogenization, bead milling, ultrasonic method, etc., whose wall breaking efficiency can reach more than 90%, but requires high equipment conditions, and must be operated at low temperature to prevent protein denaturation due to shearing and heating , affect the efficiency of enzymatic hydrolysis, only suitable for laboratory operation
[0009] In the current actual production, the combination of autolytic wall breaking and enzymatic wall breaking is the most widely used method, but this method still has many defects, such as large amount of enzyme, high enzyme cost, complicated process, etc., which greatly limits the production of yeast by-products. mass production and application of

Method used

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  • Method for producing yeast autolysate by probiotic fermentation
  • Method for producing yeast autolysate by probiotic fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Screening of strains using yeast cell walls as nutrients

[0019] 1. Medium

[0020] 1) Solid selective medium: yeast cell wall powder 1% (w / v), yeast extract 0.05% (w / v), ammonium dihydrogen phosphate 0.1% (w / v), ammonium sulfate 0.1% (w / v) , ferric chloride 0.01% (w / v), magnesium sulfate 0.01% (w / v), agar 1% (w / v).

[0021] Weigh the above components in proportion, dissolve them in tap water, adjust the pH value to 6-7, sterilize at 121°C for 20 minutes, and make a solid culture plate for use.

[0022] 2) Liquid selective medium: yeast cell wall powder 1% (w / v), yeast extract 0.05% (w / v), ammonium dihydrogen phosphate 0.1% (w / v), ammonium sulfate 0.1% (w / v) , ferric chloride 0.01% (w / v), magnesium sulfate 0.01% (w / v).

[0023] Weigh the above components in proportion, dissolve them in tap water, adjust the pH value to 6-7, and sterilize at 121°C for 20 minutes.

[0024] ,filter

[0025] Sample: Sludge from the sewage outlet of Tsingtao Brewery in ...

Embodiment 2

[0028] Example 2 Screening of broken strains

[0029] 1) Filter the yeast mud from Tsingtao Brewery with 100 mesh gauze to remove impurities, measure the moisture content, and adjust the moisture to obtain a yeast suspension with a water content of 70%-95%. The yeast cells were detected under a microscope, and the results showed that more than 99% of the yeast cells in the yeast suspension were intact and not broken.

[0030] 2) Inoculate K1, K2, K3, ..., K8 strains in 100mL liquid selective medium, 250rpm, 55°C constant temperature shaking culture for 48 hours; then add 50mL of the above yeast suspension, 250rpm, 55°C constant temperature shaking culture for 48 hours Take a small amount of culture solution every 8 hours, observe the wall breaking situation of yeast cells under a microscope, and calculate the wall breaking rate of yeast cells. At the same time, a blank control group was set up, that is, 100 mL of liquid selection medium and 50 mL of yeast suspension, withou...

Embodiment 3

[0034] Example 3 Identification of the wall-breaking strain K1

[0035] 1) Physiological and biochemical characteristics of the K1 strain:

[0036] Colonies are flat, rough and opaque surface, dirty white, spore size 0.6-0.9×1.0-1.5μm, oval or columnar, mesozoic or near-mesozoic; Gram-positive, positive for V-P test, positive for starch hydrolysis test, and indole test Positive, glucose, arabinose, xylose and mannitol can be utilized, the growth temperature range is 20-45°C, and the pH range is 5-9.

[0037] 2) Molecular biology identification of K1 strain:

[0038] The K1 strain obtained from the above screening was identified by the method of molecular biology, its 16s rDNA sequence was measured, and blast comparison was performed in the GenBank nucleic acid database. Combining the biological characteristics of K1 and the 16srDNA comparison results, the applicant confirmed that the K1 strain is Bacillus subtilis ( Bacillus subtilis ), named Bacillus subtilis K1 ( Baci...

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Abstract

The invention provides a novel method for producing yeast autolysate by probiotic fermentation, which is particularly implemented by carrying out mixed fermentation on Bacillus subtilis K1 and yeast cells. The collection number of the Bacillus subtilis K1 is CCTCC NO:M2014396. After 48 hours of fermentation, the wall breaking ratio of the yeast cells in the fermentation tank filled with the Bacillus subtilis K1 is up to 95% above, thereby obtaining unexpected technical effects. Compared with the traditional technique, the method provided by the invention greatly shortens the technical process, reduces the production cost, and has obvious wall breaking effect and wide application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to a method for producing yeast hydrolyzate by fermenting probiotics. technical background [0002] Yeast, especially brewer's yeast and baker's yeast, are unicellular eukaryotic microorganisms of economic importance. Yeast cytoplasm is rich in nutrients, such as protein, ribonucleic acid, B vitamins, and rich amino acids, among which the flavor nucleotides have been widely used in food condiments and health care products, and are also widely used in animal breeding of food attractants. The β-1,3 glucan in the yeast cell wall can enhance the immune activity of mammals, reduce cholesterol and blood lipids, and promote wound healing, etc. It is a good biological effect regulator. Because the yeast cell wall is very thick, the above-mentioned substances cannot be released to play a role without breaking the complete yeast cell. Therefore, how to break the yeast cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12R1/645
CPCC12N1/063
Inventor 苟万里魏万权李冬冬陈琳
Owner 青岛玛斯特生物技术有限公司
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