Extraction method of Cinnamomum camphora anthocyanin

An extraction method and technology of camphor fruit are applied in the field of high-efficiency extraction of plant anthocyanins, and can solve problems such as the influence of low-efficiency energy consumption on biologically active substances and the like

Active Publication Date: 2021-08-13
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with other extraction techniques, the energy provided to the material during ultra-high pressure treatment is relatively low, and generally only destroys non-covalent bonds such as hydrogen bonds, ionic bonds, and hydrophobic bonds that contribute to the three-dimensional structure of biomacromolecules, while covalent It has no effect on the bond, has the advantages of low energy consumption, high efficiency, and little impact on biologically active substances. It has been applied to the extraction of polyphenols, polysaccharides, flavonoids and other substances, but the research on ultra-high pressure assisted extraction of camphor fruit anthocyanins is still rarely reported

Method used

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  • Extraction method of Cinnamomum camphora anthocyanin
  • Extraction method of Cinnamomum camphora anthocyanin

Examples

Experimental program
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Effect test

Embodiment approach 1

[0031] The collected camphor fruit raw materials were cleaned, cored, frozen at -80°C for 24 hours, then dried in a freeze dryer for 25 hours, mechanically crushed, and passed through a 60-mesh sieve to obtain camphor fruit powder; ethyl acetate was used at a ratio of 1:2 to liquid Extract and degrease the camphor fruit powder to obtain defatted camphor fruit powder; after drying in the shade, accurately weigh 1.00 g of the defatted camphor fruit powder and mix it with 30 mL of choline chloride-acetic acid type deep eutectic solvent with a molar ratio of 1:2 , according to the proportion of 20%, after adding water to dilute, the magnetic force is fully oscillated, and the extraction temperature is 40 ℃, and the extraction is 40 minutes. Then the resulting solution was raised from atmospheric pressure to 300Mpa in a closed container over 3 minutes, and then kept under the ultra-high pressure condition for 10 minutes, and then released to atmospheric pressure over 2 seconds. Aft...

Embodiment approach 2

[0033] This embodiment is roughly the same as Embodiment 1, and the only difference is that ethyl acetate is used to extract and degrease camphor fruit powder at a solid-to-liquid ratio of 1:3. Apart from this, this embodiment is completely the same as Embodiment 1, and details are not repeated here.

Embodiment approach 3

[0035] This embodiment is roughly the same as Embodiment 2, except that after drying in the shade, 1.00 g of defatted camphor fruit powder is accurately weighed and mixed with 20 mL of choline chloride-acetic acid type deep eutectic solvent with a molar ratio of 1:2. Apart from this, this embodiment is completely the same as Embodiment 2, and will not be repeated here.

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Abstract

The invention relates to the field of chemical industry, and discloses an extraction method of Cinnamomum camphora anthocyanin. The method comprises the following steps of: cleaning a collected Cinnamomum camphora raw material, removing kernels, carrying out freeze-drying, pulverizing, and screening to obtain Cinnamomum camphora powder; carrying out leaching degreasing treatment on the Cinnamomum camphora powder by using ethyl acetate according to a certain material-liquid ratio to obtain degreased Cinnamomum camphora powder; mixing the degreased Cinnamomum camphora powder and a deep-eutectic solvent according to a certain material-liquid ratio, diluting with water, and carrying out magnetic sufficient oscillation extraction; carrying out ultrahigh-pressure treatment on the obtained solution under ultrahigh-pressure conditions; adding a certain proportion of back-extraction solution into the obtained solution, centrifuging, and taking the supernate to obtain a Cinnamomum camphora anthocyanin crude extract; and eluting the Cinnamomum camphora anthocyanin crude extract with a macroporous resin and a dextrangel to obtain a Cinnamomum camphora anthocyanin refined extract. The degreasing and deep-eutectic solvent repeated extraction are matched with the ultrahigh-pressure treatment to achieve the excellent wall breaking effect, thereby greatly enhancing the extraction efficiency of the Cinnamomum camphora anthocyanin.

Description

technical field [0001] The invention relates to the field of high-efficiency extraction of plant anthocyanins, in particular to a method for extracting camphor fruit anthocyanins. Background technique [0002] Anthocyanins are natural water-soluble pigments that widely exist in nature. They exist in the cell fluid of flowers, fruits, stems, leaves and root organs of plants, making them appear in different colors from yellow, red, purple, blue, black, etc., with Antioxidant, protect cardiovascular, improve eyesight, maintain the diversity of intestinal flora, etc., and can significantly enhance the body's immunity, improve the body's resistance to pathogenic bacteria and viruses, and protect the body's health. Anthocyanins have a wide range of sources and abundant reserves, among which plants are the main source of natural anthocyanins, and the number of plant species in my country ranks third in the world, providing diverse and rich sources for the extraction and application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/54B01D11/02A61K131/00
CPCA61K36/54B01D11/0211B01D11/0288B01D11/0292B01D11/02A61K2236/37A61K2236/17A61K2236/15A61K2236/55Y02P20/54
Inventor 赵祥杰宋毓晔聂泽坤陈曦仝争杨荣玲毕艳红赵希荣
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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