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Method for extracting marine red shrimp astaxanthin from rhodotorula mucilaginosa

A technology of sea red astaxanthin and plastic red yeast, which is applied in the field of sea red astaxanthin extraction, can solve the problems of inability to meet market demand and high price, and achieve the effect of maintaining integrity and ensuring stability

Inactive Publication Date: 2015-08-19
WEIHAI LIDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many biotechnology companies in the United States, Canada, the European Union and other countries are committed to developing and producing such products, but they are far from meeting market demand and the price is high

Method used

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  • Method for extracting marine red shrimp astaxanthin from rhodotorula mucilaginosa
  • Method for extracting marine red shrimp astaxanthin from rhodotorula mucilaginosa
  • Method for extracting marine red shrimp astaxanthin from rhodotorula mucilaginosa

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Preparation of Rhodotorula japonicum cells: Weigh 3500 g of Rhodotorula japonicus fermentation liquid, centrifuge for 15 minutes, discard the supernatant, wash with distilled water and centrifuge again, and repeat the operation 3 times to obtain Rhodotorula japonicus cells.

[0026] Accurately weigh 8 g of Rhodotorula gelatinum thalline (wet bacterium slime), add 40 ml of water (substrate concentration 20%), add β-glucanase (enzyme activity 10000U / g) and β-mannanase (enzyme activity 60000U / g) each 0.2g (0.005g / ml×40ml=0.2g), adjust the pH to 4.5, the reagents used to adjust the pH value are hydrochloric acid and sodium hydroxide solution, 50 ℃ water bath for 1h, then centrifuge at 4200rpm for 25min, discard The cell wall of Rhodotorula supernatant was broken, and the cell was collected. In the present invention, the enzymatic method is used to break the wall thoroughly, and the effect of breaking the wall is obviously better than that of the chemical method, which is be...

Embodiment 2

[0032] Preparation of Rhodotorula japonicum cells: Weigh 4000 g of Rhodotorula japonicus fermentation broth, centrifuge for 15 minutes, discard the supernatant, wash with distilled water and centrifuge again, and repeat the operation 4 times to obtain Rhodotorula japonicus cells (wet sludge).

[0033] Accurately weigh 9 g of Rhodotorula gelatinum thalline (wet bacterium slime), add 30 ml of water (substrate concentration 30%), add β-glucanase (enzyme activity 10000U / g) and β-mannanase (enzyme activity 60000U / g) each 2g (0.05g / ml×40ml=2g), adjust the pH to 5.0, bathe in water at 50°C for 2h, then centrifuge at 4200rpm for 25min, discard the supernatant rhodotorula and collect the broken cells of rhodotorula. wall bacteria, the broken wall is as follows Figure 1a-Figure 1b shown.

[0034] Add 90ml of absolute ethanol to the broken cell of Rhodotorula colloides, the mass volume ratio of the cell to absolute ethanol is 1:9, mix thoroughly, and extract at 25°C for 25 minutes;

[...

Embodiment 3

[0039]Accurately weigh 8 g of Rhodotorula gelatinum thalline (wet bacterium slime), add 40 ml of water (substrate concentration 20%), add β-glucanase (enzyme activity 10000U / g) and β-mannanase (enzyme activity 60000U / g) 0.2g each, adjust the pH to 4.8, bathe in 50°C water for 2h, then centrifuge at 4200rpm for 20min, discard the supernatant broken cells of Rhodotorula japonicus, and collect the broken cells of Rhodotorula japonicus.

[0040] Add 50ml of absolute ethanol to the broken cell of Rhodotorula japonicus, the mass volume ratio of the cell to absolute ethanol is 1:5, mix thoroughly, and extract at 26°C for 20 minutes;

[0041] Ultrasonic further extraction is used to obtain the crude extract of sea red astaxanthin, such as figure 2 shown. Ultrasonic conditions: power 600W, ultrasonic time 8s, interval 8s, ultrasonic 90 times, time 20min.

[0042] According to the sea red astaxanthin ethanol extract: petroleum ether volume ratio of 1:1, add petroleum ether for extrac...

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Abstract

The application discloses a method for extracting marine red shrimp astaxanthin from rhodotorula mucilaginosa, which comprises the steps of breaking walls of the rhodotorula mucilaginosa, and adopting an enzymic method to break the walls of the rhodotorula mucilaginosa, wherein wall breaking conditions are as follows: Beta-glucanase with enzyme activity of 10,000U / g and Beta-mannase with the enzyme activity of 60,000U / g with the ratio of 1:1 are added, and respective enzyme adding quantities are 0.005-0.05g / ml, pH is regulated to be 4.5-5.0, and water bath is performed at 50 DEG C to obtain the wall-broken rhodotorula mucilaginosa; digestion with absolute ethyl alcohol; ultrasonic extraction; removing polar impurities; obtaining the product. According to the method, the wall breaking is more through by adopting an enzymic method, and the wall breaking effect is obviously better than that of a chemical method, and structure completeness of marine red shrimp astaxanthin is favorably kept; the method has greater advantage in the aspect of cost, can be used for large-scale extraction and easy for implementation of industrialized production.

Description

technical field [0001] The invention relates to the technical field of yeast, in particular to a method for extracting sea red astaxanthin from Rhodotorula gelatin. Background technique [0002] Astaxanthin is a carotenoid with strong antioxidant and biological coloring ability, and has been widely used in food, medicine, cosmetics, feed and many other fields. It has high application value in preventing cardiovascular and cerebrovascular diseases, protecting eyes and central nervous system, preventing ultraviolet radiation, inhibiting tumors, anti-inflammation, and relieving exercise fatigue. [0003] Sea red astaxanthin is a carotenoid with high antioxidant capacity. Its mobility is close to that of natural astaxanthin ester in thin layer chromatography, and its antioxidant capacity is also close to that of natural L-astaxanthin. DPPH method detects that its antioxidant capacity is about 20-40% stronger than that of natural L-astaxanthin. [0004] At present, there is a g...

Claims

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Application Information

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IPC IPC(8): C07C403/24
Inventor 魏升宁张文丽孙常明李纪丰张美丽程振伟
Owner WEIHAI LIDA BIOTECH
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