Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use

A technology of Dendrobium officinale polysaccharides and Dendrobium officinale, which is applied in the direction of drug combination, pharmaceutical formulation, drug delivery, etc., can solve the problems of no polysaccharides in Dendrobium officinale, and achieve the effects of no adverse reactions, enhanced water solubility, and not easy to degrade

Active Publication Date: 2007-08-15
深圳市资福药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no medicine or pharmaceutical compos...

Method used

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  • Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use
  • Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use
  • Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] The preparation of embodiment 1 Dendrobium officinale polysaccharide extract

[0076] Take 500g of Dendrobium officinale, after sorting, crush it into a coarse powder, put it in a container, add 10 times the amount of 95% ethanol to reflux and extract twice, each time for 1 hour, recover ethanol from the filtrate, add 25 times the amount of water to extract at 80°C 4 times, 2 hours each time, the filtrates were combined, filtered, concentrated under reduced pressure to 1000ml, added 3750ml of 95% ethanol, stood overnight, filtered, recovered ethanol from the filtrate, washed the precipitate with 3 times the amount of absolute ethanol, filtered and dried, 50°C Dry under vacuum to obtain the polysaccharide extract of Dendrobium officinale. Its polysaccharide content was measured to be 44.18% (or 441.8 mg / g).

Embodiment 2

[0077] The preparation of embodiment 2 Dendrobium officinale polysaccharide extract

[0078] Take 500g of Dendrobium candidum, after sorting, crush it into a coarse powder, put it in a container, add 15 times the amount of 85% ethanol to reflux for extraction twice, each time for 1 hour, recover ethanol from the filtrate, add 25 times the amount of water to extract at 100 °C 4 times, 2 hours each time, the filtrates were combined, filtered, concentrated under reduced pressure to 1250ml, added 4700ml of 95% ethanol, stood overnight, filtered, recovered ethanol from the filtrate, washed the precipitate with 2 times the amount of absolute ethanol and acetone, and filtered dry. Vacuum drying at 50°C to obtain the polysaccharide extract of Dendrobium officinale. Its polysaccharide content was measured to be 42.73% (or 427.3mg / g).

Embodiment 3

[0079] The preparation of the dendrobium officinale polysaccharide extract of embodiment 3 purification

[0080] Take 10 g of the crude polysaccharide of Example 1 and add 500 ml of water to fully dissolve, centrifuge at 8000 r / min for 15 minutes, add 1 / 5 volume of sevage reagent to the supernatant to extract 3 times, centrifuge at 2000 r / min for 20 minutes to remove the precipitate, add an appropriate amount of After water, use an ultrafiltration membrane with a molecular weight of 10K to repeat ultrafiltration, concentrate to 200ml, and pass the concentrated solution through DEAE-52 column chromatography. The sample volume is 1 / 10 of the column volume, and deionized water and 0.1M NaCl solution are used respectively Rinse twice the column volume, detect online with anthrone sulfuric acid method, dialyze the collected first eluent and second eluent respectively, concentrate and freeze-dry to obtain two polysaccharide extracts of Dendrobium officinale with different polysacchar...

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Abstract

This invention relates to polysaccharides extract from Dendrobium candidum and its preparation method. The molecular weight of the polysaccharide extract of Dendrobium candidum is 10000-300000. The content of polysaccharide is no less than 25 wt%. The invention also relates to a Chinese medicinal composition containing polysaccharide extract from Dendrobium candidum and its preparation method, and application of the polysaccharide extract in preparing medicine for auxiliary treatment of diseases caused by hypoimmunity. The extract and Chinese medicinal composition containing the same can improve immunologic function with high bioavailability, no toxicity and adverse side effect. the preparation method is simple and convenient for operation and commercial process.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a dendrobium officinale polysaccharide extract, a preparation method and application thereof. [0002] The present invention also relates to a pharmaceutical composition, in particular to a pharmaceutical composition containing the polysaccharide extract of Dendrobium officinale and a preparation method thereof. technical background [0003] Dendrobium candidum wall.ex lindl. is a plant of the genus Dendrobium (Orchidaceae). It is recorded in the 2000 edition of the Pharmacopoeia of the People's Republic of China. It is an important traditional Chinese medicinal material in my country. Dendrobium officinale is the top grade of Dendrobium, and it ranks first among the "Nine Chinese Immortal Herbs" because of its special living environment and outstanding nourishing effect. The main components of Dendrobium candidum are polysaccharides and alkaloids. Studies have shown that...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61K9/08A61K9/14A61K9/16A61K9/19A61K9/20A61K9/48A61P37/04C08B37/00A61K31/715
Inventor 石秀伟章晓骅
Owner 深圳市资福药业有限公司
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