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Dendrobium candidum tissue water cultivating method

A technology of dendrobium candidum and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, cultivation, etc., can solve the problems of less transplanting of tissue culture seedlings, low survival rate of tissue culture seedlings, etc., and achieve more new shoots tillering, domestication The process is short and the leaves are dark green

Inactive Publication Date: 2007-09-26
XINYANG NORMAL UNIVERSITY
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Scholars at home and abroad have done a lot of work on the rapid propagation and expansion of the reproduction coefficient of Dendrobium. There have been many kinds of Dendrobium successfully cultivated tissue cultured seedlings, but there are few reports on the transplanting of tissue cultured seedlings.
At present, the low survival rate of transplanted tissue cultured seedlings is common, and the intermediate link from tissue cultured seedlings to field cultivation has become a major obstacle restricting the production and development of Dendrobium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Dendrobium candidum asexual rapid propagation technology

[0013] From April to June in spring, take the sprouts from the well-grown Dendrobium officinale plants, cut off the stems, and inoculate them in MS+BA2.0mg / L+NAA0.5mg / L+3% sucrose+0.75 % agar induction medium; the induced yellow-green protocorms were transferred into MS+BA1.0mg / L+NAA0.5mg / L+10%banana+3%sucrose+0.75%agar proliferation and differentiation medium , subculture once every 2 months or so, a large number of protocorm buds are amplified, and the quantity is determined according to the actual production situation. Then the seedlings with roots and leaves are inoculated in MS+10% banana+NAA 2.0 mg / L+3% sucrose+0.75% agar to grow strong.

Embodiment 2

[0015] Pick out the Dendrobium officinale seedlings with 5-8 roots and 8-10 leaves from the bottle, rinse the roots with clean water at 15-20°C, drain off excess water, and dry them in a shaded place to avoid wind. moisture. Then plant the Dendrobium candidum tissue cultured seedlings in the pores of a foam floating plate with a thickness of 5-10 mm, place the floating plate on the nutrient solution tank, fill the nutrient solution in the tank, immerse the root system for 40-60% of the length, and start the water pump. The nutrient solution is circulated at a flow rate of 5 m / min. Solar greenhouse or plastic greenhouse, shade and keep the light intensity at 5000-10000LX, the temperature at 15-28°C, the humidity at 80-95%, the daily management is spraying the leaves 1-3 times a day, and a small amount of ventilation. After about 30 days of hydroponic domestication, the tissue-cultured seedlings of Dendrobium officinale can perform photosynthesis well, with well-developed root ...

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Abstract

The invention discloses a method for cultivating algam Dendrobium nobile in group in water. Since the blades of algam Dendrobium nobile before output bottle will not form complete horny layer to process effective photosynthesis, according to the growth and physiological characters, the algam Dendrobium nobile output bottle is cultivated by water cultivate device, to be cultivated for 30 days at right condition, therefore, cultivated seeds can be transplanted into large field. The invention can effectively resolve the problems as low survival rate and improve survival rate to 95%.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for hydroponic domestication of dendrobium candidum tissue culture seedlings. Background technique [0002] Dendrobium candidum Wall (ex Lindl.) is one of the medicinal dendrobium recorded in the Pharmacopoeia of the People's Republic of China, and is a famous and precious medicinal plant. It has the effects of nourishing yin and clearing heat, benefiting stomach and promoting body fluid, clearing throat and improving eyesight, and enhancing human immunity. Due to excessive mining and serious damage to natural resources, it was listed as an endangered Chinese herbal medicine by the state in 1978. Dendrobium, as a commonly used traditional Chinese medicinal material, has a large market demand. How to solve the contradiction between demand and resources? The only way is artificial cultivation. Since ancient times, people have been exploring the artificial cult...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G1/00A01G7/00A01H4/00
Inventor 张苏锋远凌威陈世锋袁正仿赵昕梅张海宾李先文张伟杨薇
Owner XINYANG NORMAL UNIVERSITY
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