Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings

A technology of dendrobium candidum and tissue culture bottles, which is applied in the biological field, can solve the problems of unsatisfactory medicinal material quality, long domestication time, and long production cycle, and achieve the goals of optimizing seedling hardening and tree cultivation effects, simplifying tree cultivation methods, and reducing manpower Effect

Inactive Publication Date: 2014-02-05
GUIZHOU YANHE WUJIANG BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of rapid tree cultivation method of Dendrobium candidum tissue culture bottle seedlings, which solves the problems of long domestication t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Hardening and harvesting of seedlings:

[0035] Move the whole bottle of Dendrobium officinale tissue culture bottle seedlings from the cultivation room to the sun room for seedling hardening for 10 days, loosen the bottle cap and leave it overnight, open the bottle cap the next day, use tweezers to pinch the seedlings out of the bottle, and rinse them clean , remove and drain for 24 hours;

[0036] (2) Pretreatment of seedlings:

[0037] After the drained seedlings obtained in step (1) are sterilized and dried until the roots turn white, put the roots of the seedlings into the planting water and soak for 25 minutes, then remove and drain the water;

[0038] The planting water components are: 0.8‰ sodium p-nitrophenolate, 1.8‰ sodium o-nitrophenolate, 1.8‰ sodium 5-nitroguaiacol, 99‰ thiamine hydrochloride, 4.8‰ pyridoxine hydrochloride, cyclic 99‰ of hexanehexanol, the rest is water;

[0039] (3) Treatment of bark and moss:

[0040]Properly treat the surface of...

Embodiment 2

[0051] (1) Hardening and harvesting of seedlings:

[0052] Move the whole bottle of Dendrobium candidum tissue culture seedlings from the cultivation room to the sun room for seedling hardening for 12 days, unscrew the bottle cap and leave it for one night, open the bottle cap the next day, use tweezers to pinch the seedlings out of the bottle, and rinse them clean , remove and drain for 30 hours;

[0053] (2) Pretreatment of seedlings:

[0054] After the drained seedlings obtained in step (1) are sterilized and dried until the roots turn white, put the roots of the seedlings into the planting water and soak for 27 minutes, then remove and drain the water;

[0055] The composition of the planting water is: sodium p-nitrophenolate 1.2‰, sodium o-nitrophenolate 2.2‰, sodium 5-nitroguaiacol 2.2‰, thiamine hydrochloride 101‰, pyridoxine hydrochloride 5.2‰, cyclo 101‰ of hexanehexanol, the rest is water;

[0056] (3) Treatment of bark and moss:

[0057] Properly treat the surfa...

Embodiment 3

[0068] (1) Hardening and harvesting of seedlings:

[0069] Move the whole bottle of Dendrobium officinale tissue culture bottle seedlings from the cultivation room to the sun room for seedling hardening for 14 days, unscrew the bottle cap and leave it for one night, open the bottle cap the next day, use tweezers to clamp the seedlings out of the bottle, and rinse them clean , remove and drain for 36 hours;

[0070] (2) Pretreatment of seedlings:

[0071] After the drained seedlings obtained in step (1) are sterilized and dried until the roots turn white, put the roots of the seedlings into the planting water and soak for 29 minutes, then remove and drain the water;

[0072] The composition of the planting water is: sodium p-nitrophenolate 0.9‰, sodium o-nitrophenolate 1.9‰, sodium 5-nitroguaiacol 1.9‰, thiamine hydrochloride 99.5‰, pyridoxine hydrochloride 4.9-5.1 ‰, cyclohexyl alcohol 99.5‰, the rest is water;

[0073] (3) Treatment of bark and moss:

[0074] Appropriatel...

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PUM

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Abstract

The invention discloses a rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings. The rapid tree cultivation method is to bind dendrobium candidum tissue-culture bottle seedlings after subjected to short-term refining on threes for cultivation, and includes seedling hardening, seedling fetching, pretreatment of seedlings, treatment on tree bark and moss, preparation of a binding material, binding of the seedlings, spraying sanitization, spraying management and final-period management. According to the invention, the tree cultivation method is simplified, seedling hardening and tree cultivation effects are optimized, production period is shortened, relatively high seedling survival rate is guaranteed as the survival rate is above 0.095, the obtained medicine is high in quality, multiple effects of speediness, high yield and excellent production are achieved, and the rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings is an efficient and feasible tree cultivation method for dendrobium candidum.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid tree culture method for tissue culture bottle seedlings of Dendrobium officinale. Background technique [0002] Dendrobium officinale is one of the most precious Chinese herbal medicines in my country. It is honored as the first of the "Nine Great Immortal Grasses of China". It is known as "the gold in medicine". The main medicinal ingredients of Dendrobium officinale are polysaccharides, flavonoids, alkaloids, amino acids, phenols, and more than a dozen trace elements needed by the human body. At present, fresh products of Dendrobium candidum can be sold for more than 1,000 yuan per kilogram. Dendrobium candidum artificially planted in imitation of the wild has higher medicinal ingredients than dendrobium grown in greenhouses, and the market price is more than three times higher than that of ordinary dendrobium candidum. Since it is planted on the trunk, the up and down th...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G1/00
Inventor 冯新建
Owner GUIZHOU YANHE WUJIANG BIOTECH DEV
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