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Preparation method and application of dendrobium officinale extract

A Dendrobium officinale, extract technology, applied in the field of extraction and separation of plant active substances, can solve the problems of reduction, extraction rate reduction, polysaccharide components destroying water solubility, etc., and achieve simple process operation, low starch residue, and good industrial operability Effect

Active Publication Date: 2015-08-19
云南金九地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dendrobium polysaccharides in the dried dendrobium were heated to undergo intermolecular condensation, and some polysaccharide components were destroyed, and the water solubility was reduced, and the extraction rate was reduced.

Method used

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  • Preparation method and application of dendrobium officinale extract
  • Preparation method and application of dendrobium officinale extract
  • Preparation method and application of dendrobium officinale extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the extraction and separation method steps of this Dendrobium officinale extract are as follows:

[0025] (1) Add purified water to the fresh strips of Dendrobium candidum after net selection, mash and press, the amount of water added is 10 times the quality of the fresh strips, and filter to obtain fresh juice of Dendrobium candidum after squeezing;

[0026] (2) Add water three times the mass of the filter residue to the filter residue in step (1) and extract once at 93°C. After 40 minutes of extraction time, filter and combine the filtrate, and discard the filter residue;

[0027] (3) Combining step (1) dendrobium fresh juice and step (2) filtrate, adding neutral protease with a combined liquid mass of 0.03% to the combined liquid, incubating at 45°C for 2 hours, and then standing and fermenting at room temperature for 12 hours;

[0028] (4) Rapidly freezing the fermentation liquid in step (3) and then thawing it naturally to age and precipitate the star...

Embodiment 2

[0044] Embodiment 2: the extraction and separation method steps of this dendrobium officinale extract are as follows:

[0045] (1) Add pure water to the fresh strips of Dendrobium candidum after net selection, mash and press, the amount of water added is 11 times the quality of the fresh strips, and filter to obtain fresh juice of Dendrobium candidum after squeezing;

[0046] (2) Add water with 4 times the weight of the filter residue to the filter residue in step (1) and extract twice at 90°C. After the extraction time is 50 minutes, filter and combine the filtrate, and discard the filter residue;

[0047] (3) Combining step (1) dendrobium fresh juice and step (2) filtrate, adding neutral protease with a combined liquid mass of 0.04% to the combined liquid, incubating at 50°C for 1.5 hours, and then standing for fermentation at room temperature for 14 hours;

[0048](4) Rapidly freezing the fermentation liquid in step (3) and then thawing it naturally to age and precipitate t...

Embodiment 3

[0064] Embodiment 3: the extraction and separation method steps of this Dendrobium officinale extract are as follows:

[0065] (1) Add purified water to the fresh strips of Dendrobium candidum after net selection, mash and press, the amount of water added is 12 times the quality of the fresh strips, and filter to obtain fresh juice of Dendrobium candidum after squeezing;

[0066] (2) Add water 5 times the mass of the filter residue to the filter residue in step (1) and extract once at 95°C. After the extraction time is 60 minutes, filter and combine the filtrate, and discard the filter residue;

[0067] (3) Combining step (1) dendrobium fresh juice and step (2) filtrate, adding neutral protease with a combined liquid mass of 0.05% to the combined liquid, incubating at 60°C for 1 hour, and then standing and fermenting at room temperature for 16 hours;

[0068] (4) Rapidly freezing the fermentation liquid in step (3) and then thawing it naturally to age and precipitate the starc...

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Abstract

The invention discloses a preparation method of a dendrobium officinale extract. The method comprises the following steps: by taking fresh dendrobium officinale strips as a raw material, performing fermentation with neutral protease after juice squeezing; quickly freezing fermentation liquor, naturally unfreezing the fermentation liquor, and removing starch and other impurities from the fermentation liquor; removing a starch solution through treatment with strongly acidic cation exchange resin and weakly basic anion exchange resin; and finally performing concentration with a titanium stick filter, performing alcohol precipitation and centrifugation, collecting supernatant, and freeze-drying the supernatant to prepare the dendrobium officinale extract. The preparation method is simple to operate, low in cost and high in industrialization degree. The dendrobium officinale extract provided by the invention is used for preparing medicines for treating wounds. Compared with a control group, the dendrobium officinale extract is remarkably different in wound size, healing speed and mean healing time within 10 days after operation of rabbits; and the dendrobium officinale extract can speed up the healing of a wound of a wound model rabbit.

Description

technical field [0001] The invention relates to a preparation method and application of an extract of dendrobium officinale, belonging to the field of extraction and separation of plant active substances. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo) is a plant of the genus Dendrobium orchidaceae, which is a perennial epiphytic herb. [0003] Dendrobium candidum is listed as the top grade in "Shen Nong's Materia Medica", and is known as the first of the "Nine Chinese Immortal Grasses". Grows in high temperature and high humidity areas. The chemical components of wild Dendrobium officinale mainly include Dendrobium polysaccharides, dendrobine, bibenzyls, phenanthrenes, flavonoids, amino acid trace elements, etc. Artificial tissue culture Dendrobium candidum is mainly rich in active ingredients such as polysaccharides, and the content of dendrobine, bibenzyl, and phenanthrene is extremely low; polysaccharides ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P17/02
Inventor 蔡群虎田红
Owner 云南金九地生物科技有限公司
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