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Method for producing dendrobium officinale tissue culture mycorrhizal seedlings

A Dendrobium officinale and production method technology, applied in the field of tissue cultured mycorrhizal seedling production of Dendrobium officinale, can solve the problems of lack of mycorrhizal fungi, low survival rate, easy to infect miscellaneous bacteria, etc., achieve more new shoots, tillering, and large growth , the effect of plant internode expansion

Inactive Publication Date: 2011-08-10
梁经军
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Benefits of technology

This patented technology improves the ability for culturing cells called dendrocyte moldans (DM) from different sources such as Trichoderma spp., Rhodobactor brevis or Plectranthus nigrescens. These techniques help promote cellular division during plantation while maintaining their strength against external pressure like water flow. By growing these seeds into roots instead of just grains that were taken out of soil, this process increases the number of adventitious shoots on each stem compared to traditional methods where multiple bulky cloned germinants had been used previously. Overall, it provides technical benefits over previous treatments which resulted in better crop yields.

Problems solved by technology

This patented technical problem addressed by these inventors relates to finding ways to improve the quality and quantity of certain types of herbs that can be consumed without causing harmful side reactions like snake bitings.

Method used

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  • Method for producing dendrobium officinale tissue culture mycorrhizal seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1) Culture of mycorrhizal fungi

[0022] In this example, the mycorrhizal fungus LP1 was used to activate once the strains in inclined test tubes stored at low temperature, and then transferred to PDA plates, and cultured at a constant temperature of 25°C for 12 days. The edge is perforated into a bacterial sheet, and the solid medium is inserted into a small piece;

[0023] The fungal strain culture adopts solid culture, and the solid medium material of mycorrhizal fungus is mixed with cottonseed hulls and broad-leaved tree sawdust in a weight ratio of 1:1, and the amount of water added is 170 wt% of the raw material; The g medium was connected with 2 bacterial slices (Φ=8 mm); the fungus was cultured in the dark with an Erlenmeyer flask, and when most or all of the hyphae were covered with the medium, it was taken out to obtain solid mycorrhizal fungi;

[0024] (2) Culture of mycorrhizal bacteria

[0025]Prepare nitrogen-free medium: glucose 10 g / L, NaCl 0.2 g / L, K...

Embodiment 2

[0035] The basic operation of this embodiment is the same as that of Embodiment 1, the difference is:

[0036] 1. The bacterial strain LP1 fungal strain used in this embodiment does not adopt the DP6 bacterial strain.

[0037] 2. Mycorrhizal fungi adopt liquid culture medium. Preparation of medium: prepared with water, glucose 20 g / L, KH 2 PO 4 20 g / L, MgSO 4 1.5 g / L, vitamin B 1 10 mg / L, natural product: wheat bran 30 g / L (boiled juice), medium pH=6.0. After autoclaving, the above-mentioned fungi were inserted, and 2 bacterial slices (Φ=8 mm) were inserted into each 100 mL of medium. The fungus was cultivated in the dark with shaking and aeration in the Erlenmeyer flask with a filling capacity of 50% and a rotational speed of 100 rpm, and was cultured at 25°C for 25 days and harvested. After the culture is finished, the culture product is crushed with a homogenizer to obtain liquid mycorrhizal fungi.

[0038] During the production of Dendrobium candidum tissue-cultu...

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Abstract

The invention relates to a method for producing dendrobium officinale tissue culture mycorrhizal seedlings, which comprises the following steps of: (1) culturing mycorrhizal fungi by using a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP1, namely Chaetomium sp.; (2) culturing mycorrhizal bacteria, wherein the mycorrhizal bacteria DP6 are Azotobacter sp.; (3) culturing the dendrobium officinale tissue culture seedlings; and (4) carrying out water culturing and training on the tissue culture seedlings. With the method for producing the dendrobium officinale tissue culture mycorrhizal seedlings, provided by the invention, not only the growth of the dendrobium officinale tissue culture seedlings is promoted, but also the tissue culture seedlings are obviously thickened. After transplantation, the survival rate can reach more than 95%, and the tissue culture seedlings have high growth rate, great bud tillering rate, rapid growth rate and strong stress resistance. Compared with the control group of dendrobium officinale seedlings which are not inoculated to mycorrhizal fungi and bacteria to perform tissue culture, the dendrobium officinale seedlings inoculated to mycorrhizal fungi and bacteria have more prosperous root systems, obviously inflated plant internodes and high fresh weight increased by 10-20%.

Description

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Claims

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Application Information

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Owner 梁经军
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