Immune cell culture medium and application thereof

Abstract

The invention discloses an immune cell culture medium. The culture medium is prepared from the following components: anhydrous calcium chloride and other multiple inorganic salts, L-leucine and othermultiple amino acids, interleukin 2, interleukin 4 and the like. The immune cell culture medium can be used for remarkably increasing the proliferation rate of cells, greatly prolonging days for culturing cells and effectively keeping expression of surface markers when being used for culturing CAR-T cells, and has the advantages of long preservation period, easy preparation and the like.

Description

technical field [0001] The invention relates to an immune cell culture medium and its application in culturing CAR-T cells, belonging to the field of biomedical technology. Background technique [0002] Tumor-targeted immunotherapy represented by chimeric antigen receptor (CAR)-modified T cells has shown good targeting, killing and persistence in vitro and in clinical trials, demonstrating great application potential and Prospects. The principle of CAR-T cell therapy is that T cells modified by chimeric antigen receptors can specifically recognize tumor-associated antigens, so that the targeting, killing activity and persistence of effector T cells are higher than those of conventionally used immune cells. , and can overcome the tumor’s local immunosuppressive microenvironment and break the host’s immune tolerance state, that is, T cells expressing CAR bind to tumor antigens in an antigen-dependent, non-MHC-restricted manner, and initiate and activate a specific tumor-killi...

AI Technical Summary

Problems solved by technology

However, these media contain animal-derived components, such as fetal bovine serum, which are isolated and purified from animal serum or tissues and may contain animal pathogens, thus posing a risk of causing disease

[0005] Although products such as AIM-V of Gibco Company, Stemline of Sigma Company, GT-T551H3 of Takara Company, and TexMACS of Miltenyi Company all claim to be serum-free medium, when they are used, if serum components are not added, the lymphocyte Proliferation is relatively slow, and it is difficult to meet the number of cells required for clinical cell therapy

[0006] In addition, the existing immune cell culture medium also has the defect of short shelf life, which will lead to a sharp decline in cell proliferation efficiency in the later stage of the CAR-T cell culture cycle.

Images