Method for directly inducing and quickly propagating bog bilberry root nodule in test tube

A technology of bilberry root nodules and bilberry buckwheat is applied in the field of plant reproduction to achieve the effects of shortening the subculture proliferation cycle, the method is simple and the operability is strong

Active Publication Date: 2010-09-08
TONGHUA NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this study, the direct induction of root nodules and the high-efficiency and rapid propagation of root-nodulated seedlings in Vaccinium baicalensis in test tubes have not been reported at home and abroad so far.

Method used

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  • Method for directly inducing and quickly propagating bog bilberry root nodule in test tube
  • Method for directly inducing and quickly propagating bog bilberry root nodule in test tube
  • Method for directly inducing and quickly propagating bog bilberry root nodule in test tube

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Embodiment Construction

[0018] The method of direct induction and rapid propagation of vaccinium vaccinium root nodules in vitro comprises the following steps:

[0019] 1 Materials and methods

[0020] 1.1 Treatment of explant materials In August, the root buds of Vaccinium vaccinium, which were short, strong and sprouted, were collected from the edge of the mixed coniferous and broad-leaved forest at an altitude of 1400m on the northern slope of Changbai Mountain. Soak in 3% sodium hypochlorite solution for 3 minutes, rinse with sterile water 8 times. Blot the surface moisture with sterile filter paper, cut off the part damaged by the sterilizing disinfectant and cut it into 1-2 leaves and 1 segment as explants for later use.

[0021] 1.2 Screening of Nodule Direct Induction Medium Place the explants in medium 1 / 4MS+KT1.50mg·L -1 Axillary buds were cultivated on the plant, and when the axillary buds grew to 2-3 cm, the axillary buds were cut from the leaf axils and transferred to 1 / 4MS as the medi...

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Abstract

The invention relates to a method for plant propagation, in particular to a method for directly inducing and quickly propagating a bog bilberry root nodule in a test tube. The method is characterized by comprising the following steps of: (1) selecting a tiller bud of a bog bilberry root as an explant, wherein a proper nutrient medium for inducing a bud root nodule is 1/4MS+IAA0.80mg.L-1+IBA0.04mg.L-1+GA30.15mg.L-1; and (2) selecting a stem node growing the root nodule already of a reproducing plant as a material to perform quick propagation in the test tube by a propagation method, cutting a seedling which contains the root nodule in the test tube from the upper part of the root and leaving 1 or 2 leaves on the cut part, cutting the cut twig into sections which have a leaf respectively, grafting the sections into the optimized root nodule induction nutrient medium to allow auxiliary buds to sprout and grow and culturing the root nodule to regenerate the root nodule. The method shortens a successive multiplication cycle, improves propagation folds, is simple, economical, practical and strongly operable, achieves the quick propagation aim and can be used for the industrial breeding of the bog bilberry having the root nodule.

Description

technical field [0001] The invention relates to a kind of plant propagation, that is, the method of direct induction and rapid propagation in test tube of Vaccinium vaccinium root nodules. Background technique [0002] In the prior art, Vaccinium uliginosum Linn., also known as Dusi, Dianguo, Diguo, Dragon Fruit, Narisu (Mongolian language). Vaccinium dusi is a small deciduous shrub of the Rhododendronaceae Vaccinium genus. It is designated as a provincial-level key protected plant in the "Interim Regulations on the Protection and Management of Wild Animals and Plants of Jilin Province". It is a wild ornamental plant and a rare and endangered medicinal plant. Its main functions are heat-clearing and astringent. The fruit can be eaten raw, sweet and sour, and rich in juice. It is used as a raw material for fruit wine, fruit vinegar, jam, fruit juice and extraction of natural food coloring. It is designated as one of the five health foods by the International Food and Agricul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 顾地周冯颖姜云天朱俊义高捍东陈霞
Owner TONGHUA NORMAL UNIV
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