Method for improving tissue culture and rapid propagation proliferation rate of Fraxinus velutina

A technology of tissue culture rapid propagation and velvety white wax, which is applied in the biological field, can solve the problem of low proliferation rate of velvety white wax, and achieve the effect of increasing the multiplier and the multiplication coefficient

Active Publication Date: 2015-03-04
SHANDONG FOREST SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a method for improving the rapid pr

Method used

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  • Method for improving tissue culture and rapid propagation proliferation rate of Fraxinus velutina
  • Method for improving tissue culture and rapid propagation proliferation rate of Fraxinus velutina
  • Method for improving tissue culture and rapid propagation proliferation rate of Fraxinus velutina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Pick plump white wax seeds that are free from diseases and insect pests, peel off the seed coat and put them in a sterile glass bottle, sterilize with 70% ethanol for 20 seconds, then sterilize with 0.1% mercuric chloride for 6 minutes, and then wash with sterile water for 4 minutes times, inoculated to WPM+0.3mg L under sterile conditions -1 6-BA+4.5gL -1 Agar+30gL -1 In the sucrose germination medium (pH value 5.9), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h·d -1 (The same under the culture conditions), the seeds germinated after 7 days, and the aseptic seedlings were obtained.

[0030] Cut the aseptic seedlings into 1cm-long stems with buds, and transfer them to WPM+TDZ0.5mg·L -1 +ZT2.0mg·L -1 +6-BA0.5mg·L -1 +NAA0.5mg·L -1 +25mg·L -1 Sucrose+4mg·L -1 In the agar proliferation medium (pH value 6.0), the above-mentioned conditions were used for day and night light cycle culture, and after...

Embodiment 2

[0034] Pick plump white wax seeds that are free from diseases and insect pests, peel off the seed coat and put them in a sterile glass bottle, sterilize with 70% ethanol for 30 seconds, then sterilize with 0.1% mercuric chloride for 7 minutes, and then wash with sterile water for 5 minutes. The second time, inoculated into WPM+0.1mg / L6-BA+4g / L agar+25g / L sucrose germination medium (pH 5.8) under sterile conditions, the culture conditions were day temperature 25°C, night temperature 18°C, Light intensity 2500Lx, light time 13h·d -1 (The same under the culture conditions), the seeds germinated after 9 days, and the aseptic seedlings were obtained.

[0035] Cut the aseptic seedlings into 1.5cm-long stems with buds, and transfer them to WPM+TDZ1.5mg·L -1 +ZT1.5mg·L -1 +6-BA1.0mg·L -1 +NAA0.7mg·L -1 +30mg·L -1 Sucrose+4.5mg·L -1 In the agar proliferation medium (pH value 5.8), the above-mentioned conditions were used for day and night light cycle culture, and after 30 days, c...

Embodiment 3

[0039] Pick plump white wax seeds that are free from diseases and insect pests, peel off the seed coat and put them in a sterile glass bottle, sterilize with 70% ethanol for 25 seconds, then sterilize with 0.1% mercuric chloride for 8 minutes, and then wash with sterile water for 6 minutes. The second time, inoculated into WPM+5.0g / L agar+35g / L sucrose germination medium (pH 6.0) under sterile conditions, the culture conditions were daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h·d -1 (The same under the culture conditions), the seeds germinated after 10 days, and the aseptic seedlings were obtained.

[0040] Cut the aseptic seedlings into 2cm-long stems with buds, and transfer them to WPM+TDZ2.0mg·L -1 +ZT1.0mg·L -1 +6-BA1.5mg·L -1 +NAA1.0mg·L -1 +35mg·L -1 Sucrose+5mg·L -1 In the agar proliferation medium (pH value 5.7), the above-mentioned conditions were used for day and night light cycle culture, and after 35 days, clustered ...

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Abstract

The invention discloses a method for improving the tissue culture and rapid propagation proliferation rate of Fraxinus velutina. The method is characterized in that a WPM medium is used as a basic medium in the rapid propagation proliferation culture process of tissue culture plants, and four plant growth regulators with different concentrations are proportioned to greatly improve the propagation rate of Fraxinus velutina tissue culture plants; and a 1/2 WPM medium with harving macroelements is used as a basic medium, a combination of two auxins, a cytokinin and low sugar is used to induce rooting of the tissue culture plants in order to obtain complete plants. The method effectively solves the problem of low proliferation rate of Fraxinus velutina. Results of experiment statistics show that the proliferation rate of test tube plants improves to above 10 times from original highest 3.59 times, the proliferation coefficient improves 2-3 times, and the rapid propagation proliferation purpose of the Fraxinus velutina is realized. A large amount of seedlings can be obtained within a short time by using the method, and can fully meet the forestation need of a large area of saline-alkali soil.

Description

technical field [0001] The invention relates to a method for increasing the tissue culture and rapid propagation rate of white wax tomentosa, in particular to a method for improving the tissue culture and rapid propagation rate of white wax tomentosa by using different concentration ratios of four kinds of plant growth regulators, and belongs to the field of biotechnology. Background technique [0002] Fraxinus Velutina Torr. is a deciduous tree of the genus Fraxinus Linn. in the family Oleaceae. It is native to North America and has strong stress resistance. It is mainly used in landscaping, saline-alkali land afforestation and timber species. It was introduced to Jinan as early as 1911 (Meng Zhaohe et al., 2001), and was transferred to Tianjin in 1953 (Yang Ruixing et al., 1996). After the 1980s, it was widely cultivated in North China and East China. [0003] Soil salinization is an important factor affecting agricultural and forestry production and ecological environment...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/00A01G31/00
CPCY02A40/22Y02P60/40
Inventor 刘翠兰燕丽萍李丽王开芳夏阳王振猛
Owner SHANDONG FOREST SCI RES INST
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