Culture medium and culture method for inducing callus differentiation through peony leaves
A technology of induction medium and differentiation medium, which is applied in the field of plant tissue culture, can solve problems such as failure to form root system, stem tip propagation without dedifferentiation process, vitrification, etc., and achieve the effect of saving manpower and reducing the risk of pollution
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0020] Example 1
[0021] This research was carried out with the peony tuogui type ‘Zifengyu’ planted in the peony germplasm resource nursery of the College of Horticulture and Plant Protection, Yangzhou University:
[0022] (1) Collection time: March 28, 2014;
[0023] (2) Material selection: select young leaves;
[0024] (3) Sterilization and inoculation of leaves:
[0025] After the tender leaves are collected, use a sterilization method combining alcohol and mercury. The specific steps are as follows:
[0026] ① Rinse the young leaves in running water for 10 minutes to remove the external soil, then wash them with detergent (Shanghai Hehuangbaicat Co., Ltd.), and rinse them with running water;
[0027] ②Place the young leaves on the ultra-clean workbench, put them in a beaker, disinfect with 75% alcohol for 30s, and rinse them with sterile water repeatedly for 5-6 times;
[0028] ③Use 0.1% mercury (Shanghai Sino-Western Chemical Plant) to disinfect for 3 minutes and rinse with sterile ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap