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Establishment method of efficient regeneration system of mniochloa abersend

A technology for high-efficiency regeneration and establishment of methods, applied in the field of plant tissue culture, can solve problems such as difficult observation of offspring phenotypes, unpredictable flowering time, and distant kinship of bamboo, and achieve simple and easy implementation steps, loose implementation conditions, and excellent results. significant effect

Inactive Publication Date: 2015-11-11
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, many functional genes have been cloned from bamboo, but the verification of these functional genes is transferred to rice or Arabidopsis, which are far from bamboo. Some woody bamboos have long growth cycle and flowering time. Difficult to predict, difficult to observe offspring phenotypes

Method used

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  • Establishment method of efficient regeneration system of mniochloa abersend
  • Establishment method of efficient regeneration system of mniochloa abersend
  • Establishment method of efficient regeneration system of mniochloa abersend

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Experimental program
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Embodiment

[0034] The establishment method of moss bamboo efficient regeneration system is characterized in that it is carried out according to the following steps:

[0035] 1) Callus induction culture

[0036] Select the buds from the vigorous growth and no disease-spotted bamboo plants in the greenhouse as explants, cut off the 1.5-2.5cm stem section with buds, rinse with tap water for 1-2h, soak in 75% alcohol for 30s, and then use the mass ratio Vacuum filtration disinfection of 1% sodium hypochlorite solution for 15 minutes, rinse 5-6 times with sterile water in an ultra-clean workbench, and finally peel off the leaf sheath under a stereo microscope, cut out 0.4-0.6 cm of bud stems, one for each tube The buds were inoculated in the induction medium: the basic induction medium was MS (Murashige and Skoog), and the added exogenous hormone was 1-5mg L -1 2,4-D is 2,4-dichlorophenoxyacetic acid and 1-2mg·L -1 NAA is naphthaleneacetic acid, adjust the pH to 5.75.7; the culture conditio...

experiment example

[0046] The test relevant to the present invention and accompanying drawing will be introduced below:

[0047] Figure 1-Figure 8 , the regeneration process can be understood from the accompanying drawings and in conjunction with the corresponding descriptions, and no further introduction is made.

[0048] Figure 9 It is a comparison chart of callus and compact good callus induction of moss bamboo shoots in the following five different basic media (MS, 1 / 2MS, WPM, N6, B5) (for details of various basic media formulations, see: Wang Di , Plant Tissue Culture, Beijing: China Agricultural Press, 2004). About two weeks after the buds were inoculated on the culture medium, the buds began to expand, and calluses appeared at the bud tips around four weeks, and the callus grew well after fifty days. It can be seen from the figure that the induction rates of MS, 1 / 2MS, and WPM were not significantly different, being 75.55%, 77.78%, and 80%, respectively, but they were significantly d...

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Abstract

The invention discloses an establishment method of an efficient regeneration system of mniochloa abersend shoots. The establishment method comprises the following steps: (1) carrying out induction culture on a bud callus; (2) carrying out enrichment culture on the callus; (3) carrying out differentiation culture on the callus; (4) carrying out rooting culture of test-tube plantlets; and (5) hardening and transplanting regenerated plants. According to the invention, a plant tissue culture method taking the shoots of herbal bamboo as an explant is provided, which is a useful attempt for establishing the regeneration system by callus induction with the shoots of herbal bamboo as the explants; with the method, the callus can be efficiently induced through the explants, thus the efficient regeneration system is established, the research field of the bamboo is expanded, and good foundation is laid for the genetic transformation research of the bamboo; and moreover, the method is easy and feasible, easy to popularize, loose in implementation conditions, and remarkable in effect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically uses the buds of Bamboo japonica as explants to achieve high-efficiency regeneration through callus induction, proliferation, differentiation, test-tube seedling rooting, and transplanting, laying the foundation for its genetic transformation research . Background technique [0002] Moss bamboo ( Mniochloa Abersend ) Belongs to Poaceae (Poaceae) Bamboo subfamily (Bambusoideae) Olyreae (Olyreae) Moss genus ( Mniochloa ), belonging to the herbaceous bamboo class. There are about 88 genera and more than 1,400 species of bamboo in the world. Among them, there are 28 genera and more than 180 species of herbaceous bamboos. Most of the herbaceous bamboo species are found in Latin America. Mniochloa Because it is related to Lanternia ( Mnium ) is very similar, in 1908, Chase named it. The tissue culture of bamboo plants started in 1968, and has successively studied...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02A40/22
Inventor 林新春臧巧路王晓芹
Owner ZHEJIANG FORESTRY UNIVERSITY
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