Tissue culture fast propagation method for garcinia paucinervis

Abstract

The invention belongs to the technical field of tissue culture production and relates to a tissue culture fast propagation method for garcinia paucinervis.The method includes the following steps of 1, disinfection of an explant; 2, callus culture; 3, callus bud differentiation; 4, bud rooting.The formula of a root inducing medium is MS + 6-8g / L agar + 25-29g / L saccharose + 0.8-2.5mg / L indoleacetic acid + 0.5-1.5mg / L kinetin + 0.8-1.5mg / L activated carbon + 0.1-0.5mg / L metaxamin + 0.5-1.5mg / L compound sodium nitrophenolate + 0.3-0.8mg / L vitamin D2 + 0.15-0.35mg / L microcystin.The tissue culture fast propagation method has the advantages that propagation cost can be reduced, the growth period is short, the propagation rate is high, management is convenient, industrial production and automatic monitoring are benefited, and manpower, material resources and land for field crops are greatly saved.

Description

technical field

[0001] The invention belongs to the technical field of tissue culture production, and relates to a tissue culture and rapid propagation method of golden silk plum. Background technique

[0002] Plum chinensis is an evergreen tree with a height of 30 meters and a DBH of more than 1.5 meters; the bark is grayish brown. Cannulate opposite, leathery, oblong, 6-18cm long, 2-6cm wide, entire, apex acuminate or short acuminate, blunt, base broadly cuneate, lateral veins 5-8 pairs, below Conspicuously raised; petiole 6-15 mm long. Flowers polygamy on the same plant; male flowers in axillary and terminal cymes, sepals 4, suborbicular, petals 4, ovate, stamens numerous, filaments short, connate into 4-lobed rings, anthers 2-loculed, longitudinally fissured, Pistil nearly quadrangular, stigma peltate protruding; female flower usually solitary in leaf axils, larger than male flower, stigma peltate, middle raised, ovary spherical, about 2.5 mm in diameter, with 1 basal ...