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Affloridae haworthia succulent plant polyploidy plant cultivation formula

A technology for succulents and succulents, which is applied in the field of formulas for cultivating polyploid plants of succulents belonging to the genus succulents of the Afuhua family, can solve the problems of long time consumption and low mutagenesis rate.

Inactive Publication Date: 2021-05-07
公孙国林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional polyploid plant induction is to use colchicine to treat the vegetative organs or pollen of the plant, but this method has a low mutagenesis rate and takes a long time;

Method used

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  • Affloridae haworthia succulent plant polyploidy plant cultivation formula
  • Affloridae haworthia succulent plant polyploidy plant cultivation formula

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1 A formula for cultivating polyploid plants of the succulent plant of the genus Aphidaceae, comprising:

[0021] 1. Induction medium formula: prepared with distilled water, in which MS is the basic medium, add colchicine 0.015g / L, sucrose 30g / L, agar 9g / L, 6-benzylaminopurine 0.002g / L, 1-naphthalene Acetic acid 0.001g / L and sodium benzoate 0.75%, adjust the pH to 5.4-6.0;

[0022] Two, explant preparation: select the succulent blade that grows adventitious root, its surface is not damaged, and root system development is good.

[0023] 3. Inoculate the explants in the induction medium: inoculate the explants on the induction medium on a clean workbench, and seal it.

[0024] 4. Illumination culture: place the inoculated and sealed explants in a 20-degree light culture room for 7-20 days.

[0025] 5. Natural cultivation: After the leaves grow 1-2cm seedlings, the polyploid seedlings are naturally cultivated.

Embodiment 2

[0026] Embodiment 2 A formula for cultivating polyploid plants of the succulents of the genus Aphidaceae Twelve, comprising:

[0027] 1. Induction medium formula: prepared with distilled water, in which MS is the basic medium, add colchicine 0.01g / L, sucrose 20g / L, agar 7g / L, 6-benzylaminopurine 0.0005g / L, 1-naphthalene Acetic acid 0.0005g / L and sodium benzoate 0.5%, adjust the pH to 5.4-6.0;

[0028] Two, explant preparation: select the succulent blade that grows adventitious root, its surface is not damaged, and root system development is good.

[0029] 3. Inoculate the explants in the induction medium: inoculate the explants on the induction medium on a clean workbench, and seal it.

[0030] 4. Illumination culture: place the inoculated and sealed explants in a 20-degree light culture room for 7-20 days.

[0031] 5. Natural cultivation: After the leaves grow 1-2cm seedlings, the polyploid seedlings are naturally cultivated.

Embodiment 3

[0032] Embodiment 3 A formula for cultivating polyploid plants of the succulents of the genus Aphidaceae Twelve, comprising:

[0033] 1. Induction medium formula: prepared with distilled water, in which MS is the basic medium, add colchicine 0.01g / L, sucrose 25g / L, agar 8g / L, 6-benzylaminopurine 0.02g / L, 1-naphthalene Acetic acid 0.0005g / L and sodium benzoate 0.5%, adjust the pH to 5.4-6.0;

[0034] Two, explant preparation: select the succulent blade that grows adventitious root, its surface is not damaged, and root system development is good.

[0035] 3. Inoculate the explants in the induction medium: inoculate the explants on the induction medium on a clean workbench, and seal it.

[0036] 4. Illumination culture: place the inoculated and sealed explants in a 20-degree light culture room for 7-20 days.

[0037] 5. Natural cultivation: After the leaves grow 1-2cm seedlings, the polyploid seedlings are naturally cultivated.

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Abstract

The invention relates to the technical field of plant cultivation, in particular to an affloridae haworthia succulent plant polyploidy plant cultivation formula. The affloridae haworthia succulent plant polyploidy plant cultivation formula comprises the following raw materials including an MS culture medium, an inducer, cane sugar, a coagulator, an accelerant, a regulator and a bactericide. According to the affloridae haworthia succulent plant polyploidy plant cultivation formula, strict sterile operation is not needed, the production cost is reduced, meanwhile, the pollution rate of explants is effectively controlled, the mutagenesis rate of plants is increased, the mutagenesis time is shortened, the polyploidy plants can be produced on a large scale within a short time, the obtained polyploidy plants do not need to be subjected to a hardening-seedling stage of tissue culture, and the consistency of the plants is good.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a formula for cultivating polyploid plants of succulent plants of the genus Aphidaceae. Background technique [0002] The polyploid succulent plants of the genus Aphidaceae are shorter and sturdier than normal diploid plants, with thicker leaves, stronger growth, strong resistance to stress, and high ornamental value, but the cultivation of polyploids has become difficult; [0003] There are physical and chemical methods for polyploid plant induction, among which the chemical method is more common, with colchicine as the main inducer. The traditional polyploid plant induction is to use colchicine to treat the vegetative organs or pollen of the plant, but this method has a low mutagenesis rate and takes a long time; [0004] Through many years of experiments, the technical scheme not only improves the mutagenesis rate of the succulent plants of the genus Aphidaceae, sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/08
CPCA01H4/001A01H1/08
Inventor 杨国辉公孙国林何琼
Owner 公孙国林
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