Tissue culture seedling raising method for Hesong grass

Abstract

The invention discloses a tissue culture seedling raising method for Hesong grass. The method comprises the following steps: selecting and sterilizing Hesong grass explants, performing primary culture and germ-free shoot induction on the explants, performing subculture and proliferation on germ-free shoots, performing rooting induction and seedling development on rootless seedlings, and performing domestication and transplanting on tissue culture seedlings. Through selecting a suitable explant sterilization method, an induction medium for explant primary culture, a subculture medium for germ-free shoot culture and proliferation, a rooting medium for germ-free shoot rooting induction and medium treatment for tissue culture seedling transplanting, a practical and efficient tissue culture seedling raising technology system for Hesong grass is established. Through applying the technology system, the bacterial contamination rate of the explants can be controlled within 6%, the bud induction rate is not less than 90%, the bud proliferation rate of each generation is not less than five times, the rooting rate is up to 95%, and the tissue culture seedling transplanting survival rate is not less than 90%. The tissue culture seedling raising method is capable of solving the problems that Hesong grass is incapable of realizing sexual propagation due to no seeds, and conventional vegetative propagation such as cottage is difficult and low in efficiency.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture. Specifically, the present invention relates to a method for raising seedlings by tissue culture of pine grass. Background technique [0002] The lotus pine grass is a high-tech variety cultivated from the cells of foreign reed bamboo grassland strains, and it is the king of grasses. Pine grass is a low-cost, high-input plantation: once planted, harvested for 20 years, no need for annual cultivation; short growth cycle, high yield; high calorific value, high fiber output rate; wide adaptability, drought tolerance, Tolerant to waterlogging and barren land. It can be widely used in combustion power generation, paper making, environmental restoration, light chemical industry, feed breeding industry, etc. It has no pollen, no fruit, can not reproduce sexually, and asexual reproduction such as cuttings is also very difficult. Therefore, through the method of plant tissue culture, the q...

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Problems solved by technology

[0004] Still another object of the present invention is to provide a method for raising seedlings by tissue culture of Heliconia, aiming at the problems that Heliconia has no pollen, no fruit, can not reproduce sexually, and asexual reproduction is also very difficult, to establish a practical and efficient Heliconia Asexual Rapid Propagation Technology of Group Breeding Seedlings

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