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Proliferation medium for raspberry tissue culture and preparation method and application of proliferation medium

A proliferation medium and tissue culture technology, applied in the field of raspberry tissue culture medium and its preparation, can solve the problems of low photosynthesis efficiency, insufficient chlorophyll synthesis, weak seedlings, etc., and achieve healthy growth of seedlings, strong photosynthesis Effect of increased action efficiency and healthy leaf color

Inactive Publication Date: 2018-07-06
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: the present invention provides a kind of proliferation medium for raspberry tissue culture, aiming to solve the problem that the color of raspberry tissue culture seedlings grows in the medium, the color of the leaves is light, the synthesis of chlorophyll is insufficient, the seedlings are weak, and the photosynthesis The problem of low efficiency, improve the survival rate of raspberry tissue culture seedlings in the process of domestication

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1, the formula of present embodiment culture medium is:

[0017] Improved MS medium + 1mg / L6-BA + 0.1mg / LNAA + 31g / L sugar + 7g / L agar, with a pH of 5.7. The specific composition of each liter of medium is:

[0018] Macroelements: 1900mg potassium nitrate, 1650mg ammonium nitrate, 720mg magnesium sulfate heptahydrate (magnesium sulfate heptahydrate), 170mg potassium dihydrogen phosphate, 440mg calcium chloride dihydrate;

[0019] Iron salt: 71mg disodium edetate, 52mg ferrous sulfate heptahydrate;

[0020] Trace elements: 22.3mg manganese sulfate tetrahydrate, 8.6mg zinc sulfate heptahydrate, 6.2mg boric acid, 0.83mg potassium iodide, 0.25mg sodium molybdate heptahydrate, 0.025mg copper sulfate pentahydrate, 0.025mg cobalt chloride hexahydrate;

[0021] Organic elements: 2mg glycine, 0.5mg vitamin B6, 0.1mg vitamin B1, 0.5mg niacin, 100mg inositol;

[0022] Hormone: 1mg 6-benzylaminopurine, 0.1mg naphthaleneacetic acid;

[0023] Others: agar 7g, sucrose 31g.

[002...

Embodiment 2

[0038] 1, the formula of present embodiment culture medium is:

[0039] Improved MS medium+0.8mg / L6-BA+0.15mg / LNAA+30g / L sugar+6.7g / L agar, the pH is 5.7. The specific composition of each liter of medium is:

[0040] Macronutrients: 1900mg potassium nitrate, 1650mg ammonium nitrate, 740mg magnesium sulfate heptahydrate, 170mg potassium dihydrogen phosphate, 440mg calcium chloride dihydrate;

[0041] Iron salt: 74.6mg disodium edetate, 55.6mg ferrous sulfate heptahydrate;

[0042]Trace elements: 22.3mg manganese sulfate tetrahydrate, 8.6mg zinc sulfate heptahydrate, 6.2mg boric acid, 0.83mg potassium iodide, 0.25mg sodium molybdate heptahydrate, 0.025mg copper sulfate pentahydrate, 0.025mg cobalt chloride hexahydrate

[0043] Organic elements: 2mg glycine, 0.5mg vitamin B6, 0.1mg vitamin B1, 0.5mg niacin, 100mg inositol;

[0044] Hormone: 1mg 6-benzylaminopurine, 0.15mg naphthaleneacetic acid;

[0045] Others: agar 6.7g, sucrose 30g.

[0046] 2. The configuration of the cu...

Embodiment 3

[0060] 1, the formula of present embodiment culture medium is:

[0061] Improved MS medium+0.9mg / L6-BA+0.2mg / LNAA+32g / L sugar+6.5g / L agar, the pH is 5.7. The specific composition of each liter of medium is:

[0062] Macronutrients: 1900mg potassium nitrate, 1650mg ammonium nitrate, 760mg magnesium sulfate heptahydrate, 170mg potassium dihydrogen phosphate, 440mg calcium chloride dihydrate;

[0063] Iron salt: 80mg disodium edetate, 57mg ferrous sulfate heptahydrate;

[0064] Trace elements: 22.3mg manganese sulfate tetrahydrate, 8.6mg zinc sulfate heptahydrate, 6.2mg boric acid, 0.83mg potassium iodide, 0.25mg sodium molybdate heptahydrate, 0.025mg copper sulfate pentahydrate, 0.025mg cobalt chloride hexahydrate;

[0065] Organic elements: 2mg glycine, 0.5mg vitamin B6, 0.1mg vitamin B1, 0.5mg niacin, 100mg inositol;

[0066] Hormone: 1mg 6-benzylaminopurine, 0.2mg naphthaleneacetic acid;

[0067] Others: agar 6.5g, sucrose 32g.

[0068] 2. The configuration of the cultur...

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Abstract

The invention discloses a proliferation medium for raspberry tissue culture and a preparation method and application of the proliferation medium for the raspberry tissue culture. A basic medium is a modified MS medium, the modified MS medium is obtained by adjusting the concentration of magnesium sulfate heptahydrate, disodium edetate and ferrous sulfate heptahydrate in the MS medium. After beingadjusted, the concentration of magnesium sulfate heptahydrate is 720-760 mg / L, the concentration of disodium edetate is 70-80 mg / L, and the concentration of ferrous sulfate heptahydrate is 52-57 mg / L.The invention further provides the preparation method and application of the proliferation culture. According to the invention, the formula of the MS medium is improved, so that leaf colors are healthier, the chlorophyll synthesis is obviously doubled, the photosynthesis efficiency is obviously increased, and the seedling grows more healthily and strongly in the growth process of raspberries in the seedling stage to greatly improve the survival rates of transplanting and domesticating raspberry tissue culture seedlings in later stage.

Description

technical field [0001] The invention relates to tissue culture, in particular to a culture medium for raspberry tissue culture and its preparation method and application. Background technique [0002] Raspberry is a high-yield economic forest tree, which needs to be planted in a large area in reality. Therefore, it is necessary to carry out tissue culture and rapid propagation of raspberry seedlings. The existing raspberry seedling tissue culture propagation method is to carry out tissue culture and rapid propagation in the laboratory to form rooted rapid propagation seedlings. The test tube cover for tissue culture is opened, and the seedlings are hardened in the indoor bottle for 2-3 days. In the greenhouse or net room, transplant the test tube seedlings into the substrate in the nutrient pot for more than 30 days, and use a shade net to shade and moisturize. Grow into desired economical seedlings. [0003] The commonly used raspberry medium formula is generally MS mediu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/00
Inventor 仇学文许建民岳冰殷悦
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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