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Induction method of cymbidium ensifolium callus

A callus and culture medium technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of easy browning and death of callus, long callus culture time, and inability to carry out subculture, etc. , to achieve the effect of not easy browning and death, shortening the cultivation time, and healthy color.

Active Publication Date: 2019-03-22
宜宾云朵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are not many studies on the induction and cultivation of Jianlan callus, and there is a problem of difficulty in induction. The induced callus is easy to brown and die, and it is often impossible to subculture, or even if it can be subcultured, the callus cannot be subcultured. The cultivation time is too long, it takes more than 1 to 1.5 years, and the rooting rate is not high

Method used

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  • Induction method of cymbidium ensifolium callus
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  • Induction method of cymbidium ensifolium callus

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Take the young tender ovary of Jianlan (the normal ovary without calyx collected 35 days after artificial pollination during the flowering period) as explants, cut it into 1.5mm long ovary segments under aseptic conditions, and use 75 Disinfect the surface with % alcohol for 1min, put in 0.1g / 100ml HgCl 2 Soak in the solution for 5 minutes for disinfection, rinse with sterile water for 3 times, and dry the water;

[0035] (2) The explants are placed in the first culture medium to induce, and the first culture medium is based on 1 / 2LS, and includes the components of the following concentrations: NAA0.5mg / L, KT0.2mg / L, TDZ0.24mg / L, adjust the pH to 6.8, place at a temperature of 20°C, light 8h / d, and light intensity 1500lx for 70 days;

[0036] (3) The material cultivated in (2) is transferred to the second medium, and the second medium is based on N6, including the components of the following concentrations: NAA 0.3mg / L, ZT0.5mg / L, Inositol 10mg / L, adjust the pH to...

Embodiment 2

[0038] (1) Take the young tender ovary of Jianlan (the normally developed ovary with calyx collected 35 days after artificial pollination during the flowering period) as explants, cut it into 1 long ovary segment under aseptic conditions, and wash it with 75% alcohol Disinfect the surface for 1min, put in 0.1g / 100ml HgCl 2 The solution was soaked and disinfected for 6 minutes, rinsed with sterile water for 3 times, and dried;

[0039] (2) The explants are placed in the first culture medium to induce, and the first culture medium is based on 1 / 2LS, and includes the components of the following concentrations: NAA0.5mg / L, KT0.2mg / L, TDZ0.24mg / L, adjust pH to 6.0, place at 20°C, light 8h / d, light intensity 1500lx and cultivate for 90 days;

[0040](3) The material cultivated in (2) is transferred to the second medium, and the second medium is based on N6, including the components of the following concentrations: NAA 0.3mg / L, ZT0.5mg / L, Inositol 10mg / L, adjusted pH to 5.6, cultur...

Embodiment 3

[0042] (1) Take the young tender ovary of Jianlan (the normally developed ovary without calyx collected 25 days after artificial pollination during the flowering period) as explants, cut it into 1.2mm long ovary segments under aseptic conditions, and use 75 Disinfect the surface with % alcohol for 1min, put in 0.1g / 100ml HgCl 2 The solution was soaked and disinfected for 6 minutes, rinsed with sterile water for 3 times, and dried;

[0043] (2) The explants are placed in the first culture medium to induce, and the first culture medium is based on 1 / 2LS, and includes the components of the following concentrations: NAA0.5mg / L, KT0.2mg / L, TDZ0.24mg / L, adjust the pH to 6.3, place at a temperature of 20°C, light 8h / d, and light intensity 2000lx for 80 days;

[0044] (3) The material cultivated in (2) is transferred to the second medium, and the second medium is based on N6, including the components of the following concentrations: NAA 0.3mg / L, ZT0.5mg / L, Inositol 10mg / L, adjust th...

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Abstract

The invention discloses an induction method of cymbidium ensifolium callus. The induction method is characterized by inducing under a light culture environment by taking a tender ovary of the cymbidium ensifolium as an explant and 1 / 2LS as a first culture medium, and then continuously culturing under an alternate light-dark environment by taking N6 as a second culture medium. Callus induced and produced through the induction method disclosed by the invention is capable of realizing subculture, the callus is not easy to brown and die, the culture time is greatly shortened and is shortened from12 to 18 months to 6 to 8 months, the callus is good in growth vigor and healthy in color, and meanwhile, the inductivity is very high.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a tissue culture of orchids, in particular to a method for inducing Jianlan callus. Background technique [0002] Jianlan (Cymbidium ensifolium (Linn.) Sw.), also known as Sijilan, is a kind of ornamental plant orchid, which mainly blooms from June to October, usually in two times, and the flowering period lasts about 1 week. Mainly distributed in South China, East China and Southwest China, it has the characteristics of fragrance, high temperature resistance and easy flowering, and has high horticultural and herbal value. [0003] Jianlan is generally propagated by division. This is because the seeds of orchids are small, the embryos are undifferentiated, and there is no endosperm or only a small amount of endosperm. It is extremely difficult to germinate under natural conditions, so the method of division propagation has been used for reproduction. However, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 刁银祥
Owner 宜宾云朵生物科技有限公司
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