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A kind of induction method of Jianlan callus

A technology for callus and culture medium, applied in the field of tissue culture, can solve the problems of long callus culture time, easy browning and death of callus, inability to carry out subculture, etc. Shortening, healthy color effect

Active Publication Date: 2022-03-08
宜宾云朵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are not many studies on the induction and cultivation of Jianlan callus, and there is a problem of difficulty in induction. The induced callus is easy to brown and die, and it is often impossible to subculture, or even if it can be subcultured, the callus cannot be subcultured. The cultivation time is too long, it takes more than 1 to 1.5 years, and the rooting rate is not high

Method used

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  • A kind of induction method of Jianlan callus
  • A kind of induction method of Jianlan callus
  • A kind of induction method of Jianlan callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take the young ovary of Jianlan (the normal ovary without calyx collected 35 days after artificial pollination during flowering) as explants, cut into 1.5mm long ovary segments under sterile conditions, and use 75 % alcohol surface disinfection for 1min, placed in 0.1g / 100ml HgCl 2 The solution was soaked and disinfected for 5 minutes, rinsed with sterile water for 3 times, and the water was absorbed;

[0035] (2) The explants are placed in a first medium for induction, and the first medium is based on 1 / 2LS, and includes the components of the following concentrations: NAA 0.5 mg / L, KT 0.2 mg / L, TDZ0.24mg / L, adjusted pH to 6.8, placed at 20°C, lighted for 8h / d, and cultured for 70 days under the conditions of light intensity of 1500lx;

[0036] (3) Transfer the material cultured in (2) to a second medium, which is based on N6 and includes components at the following concentrations: NAA 0.3 mg / L, ZT 0.5 mg / L, Inositol 10mg / L, adjusted to pH 5.0, cultured in the dar...

Embodiment 2

[0038] (1) Take the young ovary of Jianlan (the normally developed ovary with calyx collected 35 days after artificial pollination during flowering) as explants, cut into 1-long ovary segments under sterile conditions, and use 75% alcohol Surface disinfection for 1min, placed in 0.1g / 100ml HgCl 2 The solution was soaked and disinfected for 6 minutes, rinsed with sterile water for 3 times, and the water was absorbed;

[0039] (2) The explants are placed in a first medium for induction, and the first medium is based on 1 / 2LS, and includes the components of the following concentrations: NAA 0.5 mg / L, KT 0.2 mg / L, TDZ0.24mg / L, adjusted pH to 6.0, placed at 20°C, lighted for 8h / d, and cultured for 90 days under the conditions of light intensity of 1500lx;

[0040](3) The material cultivated in (2) is transferred to the second medium, and the second medium is based on N6, including the components of the following concentrations: NAA 0.3mg / L, ZT0.5mg / L, Inositol 10mg / L, adjusted pH...

Embodiment 3

[0042] (1) Take the young tender ovary of Jianlan (the normally developed ovary without calyx collected 25 days after artificial pollination during the flowering period) as explants, cut it into 1.2mm long ovary segments under aseptic conditions, and use 75 Disinfect the surface with % alcohol for 1min, put in 0.1g / 100ml HgCl 2 The solution was soaked and disinfected for 6 minutes, rinsed with sterile water for 3 times, and dried;

[0043] (2) The explants are placed in the first culture medium to induce, and the first culture medium is based on 1 / 2LS, and includes the components of the following concentrations: NAA0.5mg / L, KT0.2mg / L, TDZ0.24mg / L, adjust the pH to 6.3, place at a temperature of 20°C, light 8h / d, and light intensity 2000lx for 80 days;

[0044] (3) The material cultivated in (2) is transferred to the second medium, and the second medium is based on N6, including the components of the following concentrations: NAA 0.3mg / L, ZT0.5mg / L, Inositol 10mg / L, adjust th...

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Abstract

The invention discloses a method for inducing Jianlan callus. The method takes Jianlan young tender ovary as an explant, uses 1 / 2 LS as the first culture medium, induces it under a bright culture environment, and then uses N6 as the second culture medium. Second culture medium, continue to cultivate in the environment of alternating dark and light, the callus induced by this method can realize subculture, the callus is not easy to brown and die, and the culture time is greatly shortened, from 12 to 18 months to After 6 to 8 months, the callus grows well, has a healthy color, and has a high induction rate.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, in particular to a tissue culture of orchids, in particular to a method for inducing orchid callus. Background technique [0002] Cymbidium ensifolium (Linn.) Sw., also known as Four Seasons Orchid, is a kind of ornamental orchid. It mainly blooms from June to October, usually in two times, and the flowering period lasts about 1 week. Mainly distributed in South China, East China and Southwest China, it has the characteristics of fragrance, high temperature resistance and easy flowering, and has high horticultural and herbal value. [0003] Jianlan is generally propagated by division. This is because the seeds of orchid are small, the embryo is undifferentiated, and there is no endosperm or only a small amount of endosperm. It is extremely difficult to germinate under natural conditions, so the method of division propagation has been used for propagation. However, this method of reproduct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 刁银祥
Owner 宜宾云朵生物科技有限公司
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