A special medium for patchouli rooting culture and its preparation method and application

Abstract

The invention discloses a special culture medium for rooting of patchouli and its preparation method and application. The special medium for rooting of patchouli comprises the following components: Huabao No. 1, Huabao No. 2, ferrous sulfate, and ethylenediamine Disodium tetraacetate, sodium molybdate, manganese sulfate, zinc sulfate, copper sulfate, cobalt chloride, boric acid, α-naphthaleneacetic acid, activated carbon, nano-silver solution, penicillin, streptomycin and carrageenan. It can be used directly after high-temperature disinfection; use the special medium for patchouli rooting culture to carry out rooting and seedling cultivation of patchouli tissue culture seedlings, and directly carry out in the natural environment of plastic greenhouses by sealing with plastic wrap and controlling environmental factors during the tissue culture process Rooting and hardening seedlings can greatly reduce the pollution rate during rooting and hardening seedlings, and reduce the time for hardening seedlings. In combination with the special medium for rooting and cultivating patchouli in the present invention, it can effectively shorten the process of planting seedlings in the tissue culture of patchouli. The growth cycle is fast, the reproduction rate is high, the transplanting survival rate is high, the pollution rate is low, and the comprehensive cost is low.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a special culture medium for rooting of patchouli and its preparation method and application. Background technique [0002] Patchouli [Pogostemon cablin ( Blanco ) Benth ] is a perennial herb or semi-shrub of the Lamiaceae Patchouli genus, and its dry aboveground part is the traditional Chinese medicine Patchouli. Patchouli is one of the main authentic medicinal materials in Guangdong Province. It is recorded in the "Chinese Pharmacopoeia" that it has the effects of aromatizing turbidity, stopping vomiting, and relieving summer heat. Patchouli is originally produced in the Philippines, Malaysia and other countries. It was first introduced into my country in the Song Dynasty. Due to different ecological conditions such as climate, patchouli rarely blooms and sets seeds after being introduced into my country, so long-term use of cuttings for propagation results in slow plant growth, h...

AI Technical Summary

Problems solved by technology

However, these mediums are generally MS medium with a certain amount of different plant hormones added, but due to the strict control of the country on the use of flammable and explosive chemicals, the nitrates in the MS medium components are listed as controlled The range makes the operation of the plant tissue culture industry difficult, and the organic matter and sucrose components contained in the MS medium are likely to cause the pollution of the medium and increase the cost of the medium. Therefore, find other chemical substitutes and avoid these The use of nitrates is the key technology and effective method to solve this major problem faced by the plant tissue culture industry. At the same time, the current tissue culture medium must be sterilized at high temperature when used, and the rooting and hardening of tissue culture seedlings The treatment of the process has a significant impact on the survival rate and growth of patchouli seedlings, but there is no report

Images