Haworthia maughanii in vitro culture method
An in vitro culture, twelve-volume technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as long flowering time, high browning rate and pollution rate, and long explant cycle. , to achieve the effect of shortening the transfer time, reducing the difficulty of obtaining materials, and making the regenerated seedlings robust.
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Embodiment 1
[0021] The leaves of "Daoqi" in Mao Han Twelve Volumes were selected as explants. After aseptic treatment of the explants, the explants were inoculated on the primary culture callus induction medium, and placed in a suitable temperature, humidity and Cultivate under light intensity conditions to induce callus, and the specific steps are:
[0022] 1. First-generation culture: select the leaves of Maohan Twelve Volumes "Daozu" as explants, wash them with washing powder, rinse with running water for 1 hour, transfer the explants to the aseptic operating table, and disinfect them with 75% alcohol first. 10 seconds, rinse once with sterile water, then disinfect with 0.1% mercuric chloride for 5 minutes or 15% sodium hypochlorite for 15 minutes, then rinse with sterile water for 4 times, cut off the base of the leaves and cut them into 1cm 2 Small pieces of each bottle were inoculated on the callus induction medium. The callus induction medium is MS medium, and its components are 6...
Embodiment 2
[0027] The leaves of "Daoqi" in Mao Han Twelve Volumes were selected as explants. After aseptic treatment of the explants, the explants were inoculated on the primary culture callus induction medium, and placed in a suitable temperature, humidity and Cultivate under light intensity conditions to induce callus, and the specific steps are:
[0028] 1. First-generation culture: select the leaves of Maohan Twelve Volumes "Daozu" as explants, wash them with washing powder, rinse them with running water for 2 hours, transfer the explants to the sterile operating table, and disinfect them with 75% alcohol first. For 20 seconds, rinse twice with sterile water, then disinfect with 0.1% mercuric chloride for 3 minutes, then rinse with sterile water, after 6 times, cut off the base of the leaves and cut them into 2cm 2 Small pieces of each bottle were inoculated on the callus induction medium. The callus induction medium is MS medium, the components are 6-benzylaminoadenine (6-BA) 2mg / L...
Embodiment 3
[0033] The leaves of "Daoqi" in Mao Han Twelve Volumes were selected as explants. After aseptic treatment of the explants, the explants were inoculated on the primary culture callus induction medium, and placed in a suitable temperature, humidity and Cultivate under light intensity conditions to induce callus, and the specific steps are:
[0034] 1. First-generation culture: select the leaves of Maohan Twelve Volumes "Daozu" as explants, wash them with washing powder, rinse with running water for 2 hours, transfer the explants to a sterile operating table, and disinfect them with 75% alcohol first For 30 seconds, rinse twice with sterile water, then disinfect with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 6 times, cut off the base of the leaves, and cut them into 1-2cm 2Small pieces of each bottle were inoculated on the callus induction medium. The callus induction medium is MS medium, and the components are 6-benzylaminoadenine (6-BA) 3mg / L, kinetin ...
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