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Trichoderma haworthia YW411, culture method, microbial inoculum and application of trichoderma haworthia YW411

A cultivation method, technology of tufts, applied in the direction of application, fungicide, botanical equipment and methods, etc., can solve the problems that threaten the ginseng industry, etc.

Active Publication Date: 2022-03-04
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical control is mainly used for the prevention and control of plant root rot. The pesticide residues and environmental pollution caused by it seriously threaten the development of the ginseng industry. It is urgent to develop safe and environmentally friendly disease control methods to promote the sustainable, stable and healthy ginseng industry. development of

Method used

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  • Trichoderma haworthia YW411, culture method, microbial inoculum and application of trichoderma haworthia YW411
  • Trichoderma haworthia YW411, culture method, microbial inoculum and application of trichoderma haworthia YW411
  • Trichoderma haworthia YW411, culture method, microbial inoculum and application of trichoderma haworthia YW411

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Isolation and identification of Trichoderma trichoderma YW411

[0040] Using 1, 2, 3, and 4-year-old ginseng rhizosphere soil as experimental materials, 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Soil dilution; mix the above 10 -1 ~10 -6 Spread the soil dilution evenly on the PDA plate, and purify the strains by streaking on the plate. The specific steps are: after the soil dilution is applied, classify the strains according to the colony shape, etc., and pick different mycelia with the inoculation loop , Streak inoculation on a new PDA plate with the three-line method, culture at 28°C for 7 days, repeat the above operations until pure strains are obtained, and obtain 1 strain of Trichoderma tuftica, which is designated as strain I.

[0041] The biological identification of the above-mentioned strain I was carried out by optical microscope, and the colony morphology of the strain was shown in figure 1 : The colony grows rapidly, the hyphae are sparse, wh...

Embodiment 2

[0052] Embodiment 2 Trichoderma trichoderma YW411 confrontation experiment

[0053] Use a sterile inoculation loop to pick a small amount of Fusarium oxysporum (https: / / www.ddbj.nig.ac.jp / index-e.htmL, accession number is LC656545) bacteria on the slope, and put it on a new PDA plate Inoculate by streaking on the upper line, the inoculation amount is one ring, culture at 28°C for 7 days, and use a 5mm hole punch to take the bacterial block to obtain the Fusarium oxysporum bacterial block.

[0054] On one side of the PDA plate, inoculate the above-mentioned Fusarium oxysporum bacterium block with a diameter of 5 mm from the edge 1 cm, and inoculate the Trichoderma trichoderma YW411 block with a diameter of 5 mm (obtained in embodiment 1) at the same distance on the other side, at 28° C. Static culture for 7d.

[0055] The antibacterial effect is identified by plate confrontation culture. The calculation formula of the inhibition rate (IE) is: IE (%)=(1-Rc / Rs)×100%, where Rc re...

Embodiment 3

[0057] Example 3 Trichoderma trichoderma YW411 ginseng rhizosphere inoculation experiment

[0058] Preparation method of Trichoderma trichoderma YW411 fermentation broth: pick Trichoderma trichoderma strains preserved on the slant of the test tube with a sterile inoculation loop, streak on the PDA plate, culture at 28°C for 7 days, and then take 5 bacteria with a diameter of 5mm The blocks were inoculated in 200mL PDB medium, cultured on a shaker at 28°C and 180rpm for 7 days to obtain a fermentation broth, and the spore concentration was calculated by microscopic examination. The concentration used in the final experiment was 1.8×10 8 spores / mL.

[0059] The preparation method of the Fusarium oxysporum bacterial liquid: the preparation method is the same as the above method.

[0060] Three-year-old ginseng seedlings were used to carry out pot experiments, and 4 treatments were set up in the experiment, as follows: CK (no inoculation), T (Trichoderma trichoderma YW411 fermentat...

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Abstract

The invention relates to the field of disease control, in particular to trichoderma hairy trichoderma YW411, a culture method, a microbial inoculum and application of the trichoderma hairy trichoderma YW411. The invention provides a trichoderma haworthia YW411, and the preservation number of the trichoderma haworthia YW411 is CGMCC (China General Microbiological Culture Collection Center) No. 23202. The trichoderma haworthia YW411 disclosed by the invention can be used for effectively preventing and treating fungal diseases of plants and is free from pollution to the environment.

Description

technical field [0001] The invention relates to the field of disease prevention and control, in particular to Trichoderma trichoderma YW411, its cultivation method, bacterial agent and its application. Background technique [0002] Ginseng (Panax ginseng) is a perennial herbaceous plant belonging to Araliaceae. It is a precious Chinese herbal medicine. It has the effects of nourishing and strengthening, and preventing and curing various diseases. my country is a major producer of ginseng, accounting for about 70% of the world's total output. At this stage, logging and planting ginseng has been strictly prohibited. In the future, the development of the ginseng industry will focus on planting ginseng on high-quality farmland. However, compared with the soil for planting ginseng in forests, farmland soil has problems such as low organic matter content, poor soil fertility, small porosity, low activity of soil mineral recycling enzymes, low proportion of beneficial microorganis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14A01N63/38Y02A40/10
Inventor 赵敏杨洪岩王丽伟
Owner NORTHEAST FORESTRY UNIVERSITY
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