Haworthia cooperivar. pilfera M. B. Bayer OB-1 tissue culture formula

A technique of OB-1 and tissue culture, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as vitreous and deformed seedlings, reduce the probability of vitreous and deformed seedlings, improve yield, and quality The effect of neat specifications

Inactive Publication Date: 2016-01-13
NINGBO YINZHOU WANZHONG ECOLOGICAL ORCHARD PROFESSIONAL COOP
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] Existing Yulu tissue culture uses Yulu flower stem or flower stem ovary as explants t

Method used

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Experimental program
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Embodiment Construction

[0009] In order to have a further understanding and understanding of the structural features of the present invention and the achieved effects, the preferred embodiments are used to provide a detailed description, as follows:

[0010] An OB-1 tissue culture formula of the genus Twelfthaceae Yulu series, characterized in that MS medium is used as the basic medium, and 0.25-0.35 mg of 6-BA and 0.25-0.35 mg of KT are added to each liter of the basic medium. 0.05-0.15mg NAA, 5-6g sucrose, and adjust the pH value to 6.0, the temperature is (22±3)°C, and the light intensity is 3000lux.

[0011] Specifically, 0.3 mg of 6-BA, 0.3 mg of KT, 0.1 mg of NAA, and 5 g of sucrose are added to each liter of the basic medium.

[0012] The data shows that the probability of vitreous body and deformed seedlings in tissue culture using this formula is greatly reduced, which increases the yield of the existing technology by 30%, and the transplanted seedlings cultivated have low mortality rate and...

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Abstract

The invention relates to a Haworthia cooperivar. pilfera M. B. Bayer OB-1 tissue culture formula. A MS medium is used as a basic medium, 0.25-0.35mg of 6-BA, 0.25-0.35mg of KT, 0.05-0.15mg of NAA and 5-6g of cane sugar into each liter of the basic medium, pH of the mixture is adjusted to 6.0, the temperature of the mixture is adjusted to 22+/-3 DEG C and the illumination intensity is adjusted to 3000lux. The seedling obtained by the technical scheme has a high transplant survival rate and a good form and solves the technical problem that a vitreous body and a malformed seedling can be easily produced in tissue culture.

Description

technical field [0001] The invention relates to an OB-1 tissue culture formula of the genus Twelfthaceae Yulu series. Background technique [0002] Yulu (Haworthiacooperivar.pilferaM.B.Bayer) is a soft-leaf variety of the succulents of the genus Twelve Volumes of the Agaveaceae (also known as the Eye Orchidaceae, Aloe Branch or Afuhuaceae), native to South Africa, now it can be cultivated in many places in the world. The plant is solitary at first, and then gradually grows in groups. The fleshy leaves are arranged in a rosette shape, with convex sides; the tips of the leaves are transparent or translucent, called "windows", with longitudinal dark lines on the surface and small whiskers at the top. , green leaves; loose raceme, small white flowers, with green vertical stripes. Jade dew is small and exquisite, with crystal clear leaves, carved like jade, peculiar and beautiful, like a living handicraft, very cute, decorated with small potted plants on desks, desks, window si...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 孙静忠
Owner NINGBO YINZHOU WANZHONG ECOLOGICAL ORCHARD PROFESSIONAL COOP
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