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Method for isolated culture of Haworthia succulent plants

A technology for succulents and in vitro culture, which is applied in the field of tissue culture and rapid propagation of Liliaceae twelve-volume attribute plants, can solve problems such as shortage of supply, shortage of seedlings, restrictions on application and promotion, etc., to achieve faster speed, less material, and fast breeding effect

Inactive Publication Date: 2013-07-24
上海旭东园艺有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the propagation of this plant mainly depends on ramets, and the annual reproduction is only 3-5, and there are fewer rare varieties, so the application and promotion are restricted, resulting in a shortage of seedlings, and the supply exceeds demand. The price of rare seedlings or commercial potted flowers is high, and it is difficult for ordinary people Consumption
There are some sporadic reports in China on the production of the genus Twelve, but there is no report on the in vitro culture and large-scale production of flowering branches and tender stems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The invention provides a method for in vitro cultivation of twelve volume succulents, the steps of which are:

[0027] Step 1: Selection and disinfection of explants

[0028] In spring and summer, select twelve rolls of semi-lignified flowering branches of succulent plants, cut them off from the base, wash them twice with detergent, wrap them in gauze, rinse them under the tap water for 3 hours, and drain them. Dry. Disinfect on the ultra-clean bench in the sterile room, first treat it with ethanol with a mass percentage concentration of 75% for 60 seconds, then treat it with a 1:50 mass ratio of water with Jieermin solution for 15 minutes, and finally use a mass percentage concentration of Treat with 0.1% mercuric chloride for 15 minutes and rinse with sterile water three times.

[0029] The second step: the establishment of sterile lines

[0030] Cut the sterilized flower branches into about 1 cm stem segments on the ultra-clean bench, inoculate them on the germina...

Embodiment 2

[0038] The invention provides a method for in vitro cultivation of twelve volume succulents, the steps of which are:

[0039] Step 1: Selection and disinfection of explants

[0040] In spring and summer, select twelve rolls of semi-lignified flowering branches of succulent plants, cut them off from the base, wash them twice with detergent, wrap them in gauze, rinse them under the tap water for 3 hours, and drain them. Dry. Disinfect on the ultra-clean bench in the sterile room, first treat it with ethanol with a mass percentage concentration of 75% for 60 seconds, then treat it with a 1:50 mass ratio of water with Jieermin solution for 15 minutes, and finally use a mass percentage concentration of Treat with 0.1% mercuric chloride for 15 minutes and rinse with sterile water three times.

[0041] The second step: the establishment of sterile lines

[0042] Cut the sterilized flower branches into about 1 cm stem segments on the ultra-clean bench, inoculate them on the germina...

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Abstract

The invention provides a method for isolated culture of Haworthia succulent plants, which is characterized by comprising the followings steps of: (1) selecting and disinfecting explants; (2) building a sterile system; (3) cluster bud mulitiplication of the sterile system; (4) culturing rooting; and (5) exercising seedling of test-tube plantlet. The method utilizes tissue culture technology to carry out factorization rapid propagation on the tissue culture so as to have the advantages of less material consumption, rapid propagation, purification, rejuvenation, virus free and the like, the seedling propagation speed can be fasted, and annual production of high-quality seedlings is realized. The method has a positive meaning for development of flowers with the name.

Description

technical field [0001] The invention relates to a method for tissue culture rapid propagation of liliaceae twelve-volume attribute plants. Background technique [0002] Haworthiafasciata Haw (scientific name Haworthiafasciata Haw), also known as Golden cocktail, is a genus of Liliaceae. It is native to southern Africa and is widely cultivated all over the world. There are also wild species (cold tolerance) distributed in the dry desert areas of our country. It likes a warm, dry and sunny environment, and is afraid of low temperature and dark and humid. The growth period is from March to October, the optimum temperature is 15-25 degrees, and the highest temperature can be 35-40 degrees. The soil requirements are not strict, but loose and fertile sandy loam is suitable. [0003] There are 150 species of this type of plant in the world. It is a perennial succulent herb with no stems and thick fleshy leaves. There are stripes on the leaves. Red, white, and golden stripes are d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 姚德彪顾建平
Owner 上海旭东园艺有限公司
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