Haworthia maughanii tissue culture and rapid propagation method based on LED light source

A technology of LED light source and tissue culture rapid propagation, which is applied in the field of plant breeding, can solve the problems of difficult to achieve ideal, deformed tissue culture seedlings, low proliferation rate, etc., and achieve the effect of shortening the proliferation cycle, good growth and large quantity

Inactive Publication Date: 2019-05-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method to overcome vitrified seedlings is to use solid medium, change the formula and corresponding concentration, or add additional formula to adjust osmotic pressure, but these methods vary with different plant cultures, and cannot be realized in the breeding of Maohan twelve volume seedlings , low hormone concentration will lead to low proliferation rate, high hormone concentration will lead to deformity or vitrification of tissue culture seedlings, it is difficult to achieve the desired effect by adjusting the hormone concentration

Method used

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  • Haworthia maughanii tissue culture and rapid propagation method based on LED light source
  • Haworthia maughanii tissue culture and rapid propagation method based on LED light source

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Effect test

Embodiment 1

[0029] The invention provides a method for tissue culture and rapid propagation of Twelve Volumes of Mao Han based on an LED light source. The specific steps of the method are as follows:

[0030] 1. Explant selection

[0031] Select the mature leaves of the plant type plump, strong and free of diseases and insect pests as the explants.

[0032] 2. Primary culture: disinfection and inoculation of explants

[0033] Cut the roots of the Maohan Twelve Juan plant (keep the plant intact) and wash it, wash it with washing powder, rinse it with running water for 1-2 hours, transfer the explant to the sterile operating table, and rinse it twice with sterile water first, Disinfect with 75% alcohol for 20 seconds, rinse with sterile water for 1-2 times, then disinfect with 0.1% mercuric chloride for 6 minutes, and rinse with sterile water for 6-8 times, then peel off the leaves, which should have a base tissue, the leaves were directly connected to the callus induction medium, and ...

Embodiment 2

[0045] The invention provides a method for tissue culture and rapid propagation of Twelve Volumes of Mao Han based on an LED light source. The specific steps of the method are as follows:

[0046] 1. Explant selection

[0047]Select the mature leaves of the plant type plump, strong and free of diseases and insect pests as the explants.

[0048] 2. Primary culture

[0049] Disinfection and inoculation of explants

[0050] Cut the root of the Maohan Twelve Juan plant (keep the plant intact) and wash it, wash it with washing powder, rinse it with running water for 1-2 hours, transfer the explant to the sterile operating table, and rinse it twice with sterile water first, Disinfect with 75% alcohol for 20 seconds, rinse with sterile water for 1-2 times, then disinfect with 0.1% mercuric chloride for 6 minutes, rinse with sterile water for 6-8 times, peel off the leaves, and the leaves should have a base tissue, the leaves were directly connected to the callus induction mediu...

Embodiment 3

[0062] The invention provides a method for tissue culture and rapid propagation of Vientiane Vientiane based on LED light sources. The method adopts the method of selecting mature leaves of Vientiane Vientiane plants with plump plant type, robustness and no pests and diseases as explants, and the explants are sterile After the treatment, the explants were inoculated on the primary culture callus induction medium, and cultured under appropriate temperature, humidity and light intensity conditions to induce callus tissue, and induce differentiation into clustered buds under the irradiation of specific LEDs, etc. The specific steps are:

[0063] 1. Explant selection

[0064] Select the mature leaves of the plant type plump, strong and free of diseases and insect pests as the explants.

[0065] 2. Primary culture

[0066] Disinfection and inoculation of explants

[0067] Cut the root of the Maohan Twelve Juan plant (keep the plant intact) and wash it, wash it with washing po...

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Abstract

The invention discloses a haworthia maughanii tissue culture and rapid propagation method based on an LED light source. The method comprises the following steps: carrying out primary culture on a plump haworthia maughanii mature leaf as an explant; selecting red and blue LED illumination for induced differentiation and subculture; and carrying out rooting culture and domestication and transplanting to obtain a haworthia maughanii tissue culture seedling with high quality and high survival rate. According to the method, the proliferation cycle can be reduced to 28 days, the proliferation timescan be increased to 6.4, and the obtained multiple shoots are great in quantity and good in quality. The vitrification rate is reduced to 15.6%, and the obtained regrowth seedling is large in window,dwarf and robust. The plant root system is robust and healthy and the transplanting survival rate reaches 100%.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for rapid propagation of Maohan twelve volume tissue culture based on an LED light source. Background technique [0002] Mao Han Twelve Volumes (scientific name: Haworthia maughanii), also known as Vientiane and Elephant Foot Grass, is a succulent plant of the Liliaceae genus Vulgare. It likes a warm, dry and sunny environment. The fleshy leaves obliquely protrude from the base, arranged in a loose rosette shape, the leaves are semi-cylindrical, the top is truncated, translucent, and there are different patterns depending on the variety, the leaf color is dark green, gray green or reddish brown, and the surface is rough. [0003] Twelve-volume succulent is a rare variety. Due to its slow growth rate and difficult reproduction, it cannot meet the needs of the current market. It is imperative to use tissue culture and rapid propagation technology to produce the Twelve-volume ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/04
CPCY02P60/14
Inventor 李珂欣王姗鲍华鹏何洪宇刘会涛张永丽李鹏飞
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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