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Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease

A technology for amylolytic spores and plant growth, applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problems of bacteria, fungi, and arthropods, such as adverse effects, economic losses, losses, etc.

Inactive Publication Date: 2018-03-20
FMC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical agents can be used to control fungal plant pathogens, but the use of chemical agents has several disadvantages, including high cost, lack of efficacy, emergence of resistant strains of fungi, and undesired environmental impact
In addition, these chemical treatments tend to be indiscriminate and may adversely affect beneficial bacteria, fungi, and arthropods in addition to the phytopathogens targeted by the treatment
A second type of plant pest is a bacterial pathogen including, but not limited to, Erwinia spp. (e.g. Erwinia chrysanthemi), Pantoea spp. (e.g. P. .citrea)), Xanthomonas (e.g. Xanthomonas campestris), Pseudomonas spp. (e.g. P. syringae) and Ralstonia spp. (such as R. soleacearum), which can cause serious economic losses in the agricultural and horticultural industries
The last class of plant pathogens includes phytopathogenic nematodes and insects which cause severe damage and loss of plants

Method used

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  • Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease
  • Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease
  • Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease

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other Embodiment approach

[0102] Other embodiments may further include at least one of an antifreeze agent, an antifoam agent, and a microbicide.

[0103] In one embodiment there is provided a composition for promoting plant growth, the composition comprising: a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited under ATCC No. PTA-121166, or a mutant having all its identifying characteristics substances; and insecticides. Described insecticide can be one of following or their combination: pyrethroid (pyrethroids), bifenthrin, tefluthrin, ζ-cypermethrin, organophosphate, tetrachlorethylphos, chlorpyrifos (chlorpyrifos ), tebupirimphos, cyfluthrin, fiproles, fipronil, nicotinoids or clothianidin. The insecticide may include bifenthrin. The insecticide may include bifenthrin, and the composition may be in a formulation useful as a liquid fertilizer. The composition may further comprise hydrated magnesium aluminum silicate and at least one dispersant. The bifenthrin insecticide ma...

Embodiment 1

[0157] Identification of Bacterial Isolates as Bacillus amyloliquefaciens by Sequence Analysis

[0158] The plant-associated bacterial strain designated RTI474 was isolated from the roots of American ginseng grown in North Carolina. The 16S rRNA and rpoB genes of the RTI472 strain were sequenced and compared with other known bacterial strains in the NCBI and RDP databases using BLAST. The 16S RNA partial sequence (SEQ ID NO: 1) of RTI472 was determined to be compatible with Bacillus amyloliquefaciens strain NS6 (KF177175), Bacillus amyloliquefaciens strain FZB42 (NR_075005) and Bacillus subtilis subsp.subtilis (Bacillus subtilis subsp.subtilis) strain The 16S rRNA gene sequence of DSM 10 (NR_027552) was identical. In addition, the rpoB sequence of RTI472 was determined to have the highest level of sequence similarity (ie, >99% sequence identity) to the known Bacillus amyloliquefaciens AS43.3 strain; however, at the DNA level there are 10 nucleotide difference, indicating t...

Embodiment 2

[0160] Unique genes associated with lanthionine antibiotic biosynthesis in Bacillus amyloliquefaciens RTI472 strain

[0161] Further sequence analysis of the genome of the B. amyloliquefaciens strain RTI472 revealed that this strain possesses a unique gene related to lanthionine antibiotic biosynthesis, whereas a homologue of this gene is absent from other closely related B. amyloliquefaciens strains. A cluster of lanthipeptide biosynthesis was identified in Bacillus amyloliquefaciens RTI472 by computational analysis using RAST, antiSMASH and BAGEL bioinformatics platforms. RAST genome annotation identified a gene region whose protein sequence was putatively associated with lanthipeptide biosynthesis. This was confirmed by antiSMASH data, which identified the same genomic regions as those with lanthipeptide biosynthetic clusters. figure 1 A schematic representation of the genomic organization of the lanthionine antibiotic biosynthetic cluster found in B. amyloliquefaciens R...

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Abstract

Compositions and methods include a new strain of Bacillus amyloliquefaciens having activity against plant pathogens. The compositions are useful for benefiting plant growth and / or conferring protection against a pathogenic infection when applied to plant foliage, flowers, fruits, bark, roots, seeds, callus tissue, grafts, cuttings, surrounding soil or growth medium, and soil or growth medium concomitant with sowing seed and planting callus tissue, grafts, and cuttings. The compositions containing the Bacillus amyloliquefaciens RTI472 strain ca n be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, or fertilizers. In one example, the Bacillus amyloliquefaciens RTI472 strain can be delivered to the plant as part of an integrated pest management program, with other microbial or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, and plant growth regulators.

Description

[0001] Related application cross-references [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 097,207, filed on December 29, 2014, the disclosure of which is incorporated herein by reference in its entirety. Technical field [0003] The presently disclosed subject matter relates to isolates comprising Bacillus amyloliquefaciens RTI472 for application to plant leaves, plant fruits and flowers, plant seeds and roots, and the soil surrounding the plants to facilitate plant growth and treat plant diseases. combination. Background technique [0004] It is known that many microorganisms with beneficial effects on plant growth and health are present in the soil, living with the plants, especially in the root zone (Plant Growth Promoting Rhizobacteria "PGPR"), or as endophytes Live in plants. Their beneficial plant growth promoting properties include nitrogen fixation, iron chelation, phosphate solubilization, inhibition of non-beneficial m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N43/40A01N43/653A01N53/00A01P3/00A01P21/00A01N63/22
CPCA01N43/40A01N43/653A01N63/10A01N37/46A01N63/22A01N25/24A01N65/20A01N25/02A01N25/12A01N25/30C12N1/20A01C1/06A01G7/06A01N53/00C12R2001/07C12N1/205
Inventor S·塔加维D·范德乐列M·A·R·希林斯基J·李
Owner FMC CORP
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