The present invention relates to the field of
genetic engineering,
protein engineering technology and its application, and discloses a novel recombinant collagen and its application, including any one of the recombinants COL17-CO-1, COL17-CO-2, COL17-CO-3 or COL17-CO-4 of the specific functional region of
human type XVII collagen and the 1232-1466 region of
human type III collagen, wherein the
amino acid sequences of the specific functional regions in the COL17-CO-1, COL17-CO-2, COL17-CO-3 and COL17-CO-4 are SEQ ID No.2, SEQ ID No.6, SEQ ID No.8 and SEQ ID No.10, respectively. The recombinant exhibits better ability to promote
cell proliferation, promotes the expression of
proline hydroxylase (HYP), improves the
antioxidant capacity of
skin cells, improves the expression of multiple collagens in
skin cells, and better reduces the expression of
collagen degradation-related bases MMP-3 and MMP-9.