Tissue culture method of haworthia retusa

A technology of tissue culture and blue bird life, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficulty in rapid reproduction, few materials, and low success rate.

Active Publication Date: 2015-07-08
NANJING XIAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some high-end ornamental succulents are relatively scarce, and there are few materials. Plant tissue culture is limited by the source of materials, and the success rate is low
Difficult to reproduce rapidly, resulting in prolonged production cycles
At present, there is no report on the research on the tissue culture and rapid propagation system of Qingniaoshou

Method used

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  • Tissue culture method of haworthia retusa
  • Tissue culture method of haworthia retusa
  • Tissue culture method of haworthia retusa

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for tissue culture of Jade Bird Shou, comprising the steps of:

[0026] 1) Materials: use the female parent of Jade Bird Shou (such as figure 1 Shown) fleshy leaves as explants, use cotton thread to strip the selected Jade Bird Shou plant leaves into individual leaves, first clean the Jade Bird Shou leaves with a detergent containing 60% linear alkylbenzene sulfonate sodium, and pour them out after cleaning. Remove the washing liquid, then rinse the leaves washed with washing powder under tap water for 30 minutes, put the leaves at 4°C overnight, and rinse the leaves under running water for 2 hours the next day.

[0027] 2) Sterilization of materials: Place the explants of Jade Bird Shou that have been cleaned above in a beaker on a clean bench and soak them in 75% alcohol for 30 seconds, rinse them with sterile water for 2-3 times after soaking, and then rinse them with sterile water. Quickly pour the cultured part of Jade Bird Shou into 0.1% mercuric acid so...

Embodiment 2

[0032] The disinfection time of mercuric chloride has a great influence on the bacterial contamination of explants. Short disinfection time will result in incomplete sterilization and serious bacterial contamination; too long disinfection time will result in excessive sterilization and browning of explants. And die. Therefore, use the optimal disinfection time to provide sterile explants for later callus induction. The experiment was carried out in three batches, each batch had the same number of inoculations, and the average of the experimental results of the three batches was taken, and Table 1 was obtained. It can be seen from Table 1 that the optimal disinfection time for mercuric chloride with a concentration of 0.1% is 5 minutes. The sterilizing result of 3 minutes is not ideal, the bacterial contamination is serious, the bacterial contamination rate reaches 100%, and the explants eventually die due to bacterial contamination; the sterilization time of 4.5 minutes can a...

Embodiment 3

[0036] Different concentrations of the same growth hormone have different effects, too high or too low concentration may cause callus non-induction behavior or cause the induction time to be too long.

[0037] The sterile explants in the MS medium were transferred to the medium of MS+6-BA 1.0 mg / L+NAA 0.1-0.4 mg / L, pH 5.8-6.0. The experiment was carried out in three batches, each batch had the same number of inoculations, and the average number of the three batches of experiments was taken, and Table 2 was obtained. It can be seen from Table 2 that NAA of 0.1mg / L and 0.4mg / L failed to induce callus, and the induction rate of NAA concentration of 0.2mg / L and 0.3mg / L could reach 50%, but it was obvious that 0.2mg / L NAA in L is the most favorable hormone concentration for callus induction, because it can grow callus in the shortest time.

[0038] Table 2 The results of different concentrations of NAA inducing the callus of Qingtiao Shou

[0039]

[0040]

[0041] The call...

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Abstract

The invention discloses a tissue culture method of haworthia retusa. The tissue culture method comprises the following steps: taking a haworthia retusa fleshy leaf as an explant; washing, sterilizing and disinfecting; then cutting the explant to the length of about 1.5cm-2cm and upwards and obliquely inserting a notch into a starting culture medium, wherein the culture temperature is 25+/-2 DEG C, the illumination time is 24h/d, and the illumination intensity is 2000lx; after the haworthia retusa explant is cultured for 7 days and the flesh of the haworthia retusa explant is gradually expanded, transferring the explant into an induction callus culture medium so as to be cultured; after 20 days, forming calluses by the explant; transferring the explant into a differential culture medium to be cultured; after about 35 days, gradually germinating and differentiating buds by the calluses; after 50 days, transferring the buds into a bud proliferation culture medium to be proliferated into budlets; gradually forming cluster seedlings by cluster buds and generating small haworthia retusa; and dividing the cluster seedlings by using a dissecting knife, continually proliferating and culturing, and putting the small haworthia retusa into pots after 70 days. According to the method, leaf flesh tissues of succulent plants are taken as the explants and a lot of plant materials can be obtained in short time, so that the succulent plants including the haworthia retusa can be rapidly proliferated and popularized.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Jade Bird Shou. Background technique [0002] Blue Bird Shou (Haworthia retusa) is a succulent plant of the genus Twelve Volumes of the Liliaceae, native to southern Africa. Because there is a window on the top of the leaf, what you see from the top is a circle composed of many triangles, just like the pattern of longevity, so it is called "Shou" from then on. There are nine kinds of Zhengshou, namely: Xishan Shou, Emperor Shou, Blue Bird Shou, Yulu Shou, Baiyin Shou, Hairy Crab Shou, Red Muscle Shou, Momen Shou, and Fangting Shou. There are many varieties of longevity. Although they are all small and round, they are all quite delicate and beautiful in appearance. They have richly varied and unique leaves, which grow slowly and have a long viewing period. Whether it is a solitary viewing or a combined bonsai, it can be maintained for a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 续晨贲爱玲蔡小宁华春王小平周泉澄陈全战赵俊丽
Owner NANJING XIAOZHUANG UNIV
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