Xinjiang snow lotus cell tissue cultured substance and method for large-scale subculture

A technology of cell tissue and subculture, which is applied in the field of plant biotechnology engineering, can solve the problems of not finding and screening high-yielding cell lines, reducing the content of active ingredients, and restricting applications, so as to achieve easy control of product quality, decrease in flavonoid content, and bacterial contamination. The effect of reducing the chance

Active Publication Date: 2007-05-16
DALIAN PRACTICAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the research report on the tissue culture of Saussurea sauraceae, there are many problems that greatly limit its application in actual production. The existing problems include: instability during the amplification process, and the content of active ingredients after the amplification to a certain scale. will decrease; there is no effective means of screening high-producing cell lines, etc.

Method used

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  • Xinjiang snow lotus cell tissue cultured substance and method for large-scale subculture
  • Xinjiang snow lotus cell tissue cultured substance and method for large-scale subculture

Examples

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Comparison scheme
Effect test

experiment example 1

[0042] Experimental example 1 Anti-inflammatory effect of the Xinjiang Snow Lotus tissue cell culture of the present invention

[0043] 1. The present invention's Xinjiang snow lotus tissue cell culture is on the influence of xylene-induced mouse ear swelling

[0044] Take mice with a body weight of 18-22g, half male and half female, and randomly divide them into five groups, 10 mice in each group. Take 100mg / kg, 150mg / kg, 200mg / kg, 300mg / kg (equivalent to the total flavonoids of snow lotus). The volume is 20ml / kg. The administration was continued for 3 days, and 1 hour after the last administration, 25 μl of xylene was evenly applied to both sides of the right ear of the mouse to cause inflammation, and the left ear was not applied as a normal control ear. After 2 hours, the mice were sacrificed by dislocation of the cervical spine, and the round ears of the same part of the left and right ears were punched out with a puncher with a diameter of 7 μm, weighed on a torque ...

experiment example 2

[0082] Experimental example 2 The analgesic effect of Xinjiang Saussurea sauraceae tissue cell culture of the present invention

[0083] 1. The present invention's Xinjiang Snow Lotus tissue cell culture is on the impact of acetic acid-induced mouse writhing reaction.

[0084] Take mice with a body weight of 18-22g, half male and half male, and divide them into six groups at random, with 10-12 mice in each group. Oral administration of 75, 150, 300mg / kg (equivalent to the total flavonoids of Snow Lotus); positive control group Indomethacin enteric-coated tablets, the dose is 10mg / kg, and the dose of Miaobi Granules is 4g / kg. The administration volume is 20ml / kg. The administration was continued for 3 days, and 1 hour after the last administration, 0.3ml / mouse of 0.8% acetic acid was injected intraperitoneally, and the number of writhing of the mice within 5-20 minutes after the injection of acetic acid was observed.

[0085] The results showed that, compared with the vehicle...

experiment example 3

[0101] Experimental example 3 Influence of Xinjiang Snow Lotus Tissue Cell Culture of the present invention on immune function

[0102] 1, the influence of Xinjiang Snow Lotus tissue cell culture (Saul) of the present invention on mouse carbon particle clearance

[0103] Get 60 mice, half male and half female, with a body weight of 18-22 grams, randomly divided into six groups, 10 in every group, and the vehicle group was fed with the same volume of 0.5% sodium carboxymethyl cellulose, Xinjiang Snow Lotus tissue cell culture ( Saul) groups were fed with 75, 150, and 300 mg / kg of Xinjiang Saussurea chinensis tissue cell culture; the Lubi granule group was fed with 4 g / kg of Lubi granule; the indomethacin group was fed with 10 mg / kg of indomethacin , the volume is 20ml / kg. Continuous administration for 7 days; 24 hours after the last administration, 10ml / kg of Indian ink (diluted 4 times before use) was injected intravenously, 20μl of blood was taken from the orbital vein a...

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Abstract

The invention relates to a method for cultivating saussurea involucrate cell, wherein it comprises that using MS basic culture medium wit some NNA (fruitone), 6-BA (6-aminopurine) as the culture medium of callus; inducing the seed of saussurea involucrate into plant; using said plant as expalnt to be differented at special condition, to induce callus; subculturing the callus periodically, to increase the callus; using part of callus to function the seed and collecting the left to be dried as the saussurea involucrate culture; collecting said culture to be dried as the cell culture. The invention can improve the content of effective components.

Description

technical field [0001] The invention relates to a tissue culture of medicinal plants and a cultivation method thereof, in particular to a Xinjiang snow lotus tissue culture and a cultivation method thereof, belonging to the field of plant biotechnology engineering. Background technique [0002] Snow lotus is a folk medicinal plant in high mountain areas. It is used for dispelling cold and dehumidification, promoting blood circulation and stimulating menstruation, anti-inflammatory and analgesic, etc. It is mostly used in folk for rheumatoid arthritis, cold pain in women's lower abdomen, amenorrhea, retained placenta, impermeable numbness, and cold lung Treatment of cough, impotence, and altitude sickness. In recent years, Snow Lotus, as a national medicine, has attracted much attention for its anti-inflammatory and analgesic effects, anti-early pregnancy, anti-aging and inhibition of cancer cell proliferation. But my country's snow lotus is mainly distributed in the plateau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 刘汉石
Owner DALIAN PRACTICAL BIOTECH
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