A Broadband Microscopic Imaging Spectrum System and Its Variable-Wavelength Fast Focusing Control Method

Abstract

The invention discloses a broadband microscopic imaging spectrum system and a wavelength conversion rapid focusing control method thereof. The system comprises a microscope, a multiple optical paths switching device, an optical path controller, a light splitting unit, an Intensified Charge Coupled Device (ICCD), a light splitting unit controller, an image acquisition unit, a driving motor, an objective table, an objective table support, a position sensor, a light source and light splitting unit support, a focusing controller, a 2*1 optical path coupler, a reflection common light source, a reflection laser light source, a transmission common light source, a transmission laser light source, a light source controller, an image registering and controlling unit, a power supply module, and a computer. According to wavelength change, the system can automatically adjust a height of the objective table, rapid compensation of focal length change caused by the wavelength change is realized, and an acquired single wave band image can be subjected to pixel level registering. According to the invention, an imaging spectrum range is wide, ultraviolet, visible light and infrared light are covered, a microscopic image and spectrum information of a sample are obtained simultaneously, and the detection sample can be subjected to structural and functional identification and analysis.

Description

technical field [0001] The invention relates to a micro-imaging spectrum device, in particular to a wide-band micro-imaging spectrum system with the functions of variable-wavelength fast focus control and image registration. It is used in clinical diagnosis, new material analysis and other fields. Background technique [0002] The development of biology and medicine has gone through the stage of anatomical biology (histological stage) relying on appearance recognition and the stage of microscopic cell biology that goes deep into the cell level, and is currently developing to a higher stage of molecular biology, which not only requires the exploration of life from the molecular level Physiological and pathological causes and effects of diseases and diseases require an understanding of chemical and biochemical components inside and outside cells and their changes. The qualitative, quantitative and localization information and changes of the components inside and outside the c...

AI Technical Summary

Problems solved by technology

In the actual use process, this push-broom method brings two problems: one is that push-broom is a kind of mechanical movement, and the speed is relatively slow, which makes the image acquisition time longer; the other is that push-broom movement makes the operation cumbersome and reliable. Low performance, not conducive to use as general equipment

[0004] In response to these problems, Chinese patent application number: 200810036683.0, "Molecular Spectral Imager", uses an Acousto Optic Tunable Filter (AOTF) or a Liquid Crystal Tunable Filter (LCTF) as a dispersion unit to avoid The push-broom movement can realize fast imaging; the spectral range of imaging can include visible light and near-infrared; however, this system realizes spectral imaging by splitting the light source, and the measurement will inevitably be affected by ambient stray light. Detrimental effect on image quality

[0005] Although these two systems have realized the spectral imaging of microscopic objects, there are still some problems: first, the influence of wavelength change on the imaging focal length was not considered in the design of these two systems, and they failed to solve the The problem of blurring of single-band images caused by wavelength changes in spectral images; secondly, the existing similar systems did not consider the registration problem between individual single-band images after focal length changes; finally, the existing systems did not Realize simultaneous acquisition and registration of high-resolution ordinary optical imaging and spectral imaging, and adjust the wavelength range according to needs

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