Blood cell analyzer and bubble discharging method

Abstract

The invention discloses a hematology analyzer and a bubble discharging method. The hematology analyzer comprises a flowing chamber, a pressure source, a first control valve, a second control valve and a third control valve, the flowing chamber comprises a liquid containing cavity, a first liquid inlet, a second liquid inlet and a liquid outlet, the first liquid inlet, the second liquid inlet and the liquid outlet are communicated with the liquid containing cavity, the first control valve is connected with the first liquid inlet through a pipeline, and the second control valve is connected with the liquid outlet through a pipeline. The third control valve is connected with the second liquid inlet through a pipeline, and the pressure source is connected with at least one of the first liquid inlet and the second liquid inlet through a pipeline; when the first liquid exists in the liquid containing cavity, the pressure source is matched with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure for the liquid containing cavity, the liquid containing cavity is filled with liquid, second liquid is obtained, and bubbles in the second liquid are smaller than bubbles in the first liquid. Therefore, bubbles of the liquid in the flow chamber can be discharged through a simple structure.

Description

Technical Field

[0001] The present application relates to the technical field of medical detection and analysis, and in particular to a blood cell analyzer and a bubble discharge method. Background Art

[0002] Hematology analyzers are a type of instrument commonly used in medical testing among sample analyzers. They are instruments that detect parameters such as the number and proportion of blood cells (red blood cells, white blood cells, and platelets) in the blood. Hematology analyzers analyze blood to realize functions such as the type of microbial infection of the sample being tested, anemia diagnosis and treatment, and blood disease diagnosis. With the advancement of technology and the development of science and technology, the functions of hematology analyzers have been continuously expanded, the performance has been continuously improved, and the degree of automation has been continuously improved, and they have been widely used in clinical practice.

[0003] The blood cell analyzer may include equipment such as a flow chamber assembly. The blood cell analyzer may be a device that classifies and counts particles based on flow cytometry detection technology. The basic measurement principle is that after the processed sample particles (such as blood cells) are wrapped by sheath fluid, they pass through the flow chamber one by one under the push of pressure, thereby detecting the sample particles.

[0004] In order to improve the accuracy of sample detection, the flow chamber needs to be kept as free of bubbles as possible. However, when the existing flow chamber structure is used in a blood cell analyzer, there are still many bubbles in the flow chamber that adhere to the flow chamber wall or block the flow channel in the flow chamber. The presence of many bubbles in the flow chamber may cause the bubbles to be identified as sample particles during the detection process, affecting the detection results; and many bubbles in the flow chamber may affect the pressure balance of the flow channel, resulting in the inability to form a sample flow, etc. Summary of the invention

[0005] The main purpose of this application is to provide a blood cell analyzer and a bubble discharge method, aiming to solve the above-mentioned technical problems existing in the prior art.

[0006] To solve the above problems, the present application provides a blood cell analyzer, which includes: a flow chamber, a pressure source, a first control valve, a second control valve and a third control valve, the flow chamber includes a liquid containing cavity and a first liquid inlet, a second liquid inlet and a liquid outlet connected to the liquid containing cavity, the first control valve is connected to the first liquid inlet through a pipeline, the second control valve is connected to the liquid outlet through a pipeline, the third control valve is connected to the second liquid inlet through a pipeline, and the pressure source is connected to at least one of the first liquid inlet and the second liquid inlet through a pipeline; when there is a first liquid in the liquid containing cavity, the pressure source cooperates with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure to the liquid containing cavity to fill the liquid in the liquid containing cavity to obtain a second liquid, wherein the bubbles in the second liquid are less than the bubbles in the first liquid.

[0007] In some embodiments, the pressure source is connected to at least the second liquid inlet through a pipeline, and the liquid containing cavity, the pipeline connecting the flow chamber and the first control valve, the pipeline connecting the flow chamber and the second control valve, and the pipeline connecting the pressure source and the second liquid inlet are all filled with the first liquid; when the first control valve and the second control valve are set to the cut-off state and the third control valve is set to the on state, the pressure source provides negative pressure to the liquid containing cavity to enlarge at least part of the bubbles in the liquid containing cavity; when the first control valve and the second control valve are set to the on state and the third control valve is set to the cut-off state, the pressure source provides positive pressure to the liquid containing cavity and injects liquid into the liquid containing cavity through at least the second liquid inlet, so that at least part of the enlarged bubbles are broken and discharged from the liquid containing cavity, thereby filling the liquid containing cavity with the second liquid.

[0008] In some embodiments, the blood cell analyzer also includes: a controller, which is used to: control repeated execution according to a preset number of executions: when the first control valve and the second control valve are set to a cut-off state, and the third control valve is set to a conducting state, the pressure source provides negative pressure to the liquid containing cavity to enlarge at least part of the bubbles in the liquid containing cavity; when the first control valve and the second control valve are set to a conducting state, and the third control valve is set to a cut-off state, the pressure source provides positive pressure to the liquid containing cavity, and injects liquid into the liquid containing cavity through at least the second liquid inlet, so that at least part of the enlarged bubbles are broken and discharged from the liquid containing cavity.

[0009] In some embodiments, the pressure source connects the first liquid inlet and the second liquid inlet through a pipeline. When the liquid containing cavity contains the first liquid, when the third control valve and the first control valve are set to a conducting state, and the second control valve is set to a shut-off state, the pressure source provides negative pressure to the liquid containing cavity, so that the liquid in the liquid containing cavity flows out of the liquid containing cavity from the first liquid inlet at a first flow rate that is less than a preset flow rate threshold; when the first control valve and the second control valve are set to a conducting state, and the third control valve is set to a shut-off state, the pressure source provides positive pressure to the liquid containing cavity, so that the liquid flows from the first liquid inlet into the liquid containing cavity at a second flow rate that is less than the preset flow rate threshold through the pressure source, so as to fill the liquid containing cavity with liquid.

[0010] In some embodiments, the blood cell analyzer also includes a sample needle, a flow limiting tube, a first pipeline and a reaction pool connected to atmospheric pressure, the sample needle is located in the liquid containing cavity and connected to the second liquid inlet; the reaction pool is connected between the pressure source and the flow chamber; the pressure source is connected to at least the first liquid inlet, and the pressure source and the first liquid inlet are connected through the flow limiting tube and the first pipeline; when the third control valve and the flow limiting tube are set to a conducting state, and the second control valve and the first pipeline are set to a cut-off state, the pressure source provides negative pressure to the liquid containing cavity through the flow limiting tube, so that the liquid in the liquid containing cavity flows out of the liquid containing cavity from the first liquid inlet through the flow limiting tube at a first flow rate that is less than a preset flow rate threshold.

[0011] In some embodiments, the blood cell analyzer further comprises a sample needle, the sample needle is located in the liquid containing cavity and connected to the second liquid inlet, the sample needle is extended along the flow direction of the liquid in the liquid containing cavity, the flow chamber further comprises a rectifying component arranged in the liquid containing cavity, the rectifying component divides the area in the liquid containing cavity except the sample needle into a first cavity and a second cavity in the flow direction of the liquid, the first liquid inlet is connected to the first cavity, the rectifying component is provided with a rectifying hole connecting the first cavity and the second cavity; when the third control valve and the flow limiting tube are set to a conducting state, and the second control valve and the first pipeline are set to a cut-off state, the pressure source passes through the flow limiting tube A negative pressure is provided to the liquid containing cavity, so that the first liquid in the first cavity, the second cavity and the rectifying hole flows out of the liquid containing cavity from the first liquid inlet through the flow limiting tube at a first flow rate that is less than a preset flow rate threshold, thereby at least discharging the bubbles in the first cavity and the rectifying hole; when the pressure source causes the liquid to flow into the liquid containing cavity from the first liquid inlet at the second flow rate, if it is detected that the liquid enters the second cavity through the rectifying hole, the second control valve is set to a conducting state, and the liquid is caused to flow into the first cavity from the first liquid inlet at a fourth flow rate through the pressure source, so that the liquid fills the first cavity and the second cavity; wherein the fourth flow rate is greater than the second flow rate.

[0012] In some embodiments, the blood cell analyzer also includes a sample needle, which is located in the liquid containing cavity and connected to the second liquid inlet, and the sample needle is extended along the flow direction of the liquid in the liquid containing cavity, and the liquid containing cavity includes a sheath liquid cavity wrapped around the outside of the sample needle; when the liquid fills the sheath liquid cavity, the pressure source provides positive pressure to the liquid containing cavity to flow the liquid from the first liquid inlet into the liquid containing cavity at a third flow rate through the pressure source, wherein the third flow rate is greater than the second flow rate, and / or the third flow rate is greater than the fourth flow rate; when the liquid is at the third flow rate, the pressure source injects liquid into the sample needle to fill the sample needle with the liquid.

[0013] In some embodiments, the blood cell analyzer also includes a flow limiting tube and a first pipeline, and the pressure source and the first liquid inlet are connected through the flow limiting tube and the first pipeline; when the liquid fills the sheath liquid cavity, the second control valve, the flow limiting tube and the first pipeline are all set to a conducting state, and the pressure source provides positive pressure to the liquid containing cavity through the flow limiting tube, the first pipeline and the second control valve to flow into the liquid containing cavity from the first liquid inlet at the third flow rate; and when the liquid is at the third flow rate, the third control valve is set to a conducting state, and the pressure source injects liquid into the sample needle to fill the sample needle with liquid.

[0014] In some embodiments, the pressure source includes a first metering pump, which is connected to the second liquid inlet through a pipeline, and the first metering pump is used to provide positive pressure and negative pressure to the liquid containing cavity, and / or the pressure source includes a syringe, which is connected to the second liquid inlet through a pipeline, and the syringe is used to provide positive pressure and negative pressure to the liquid containing cavity; and / or, the pressure source includes a first liquid storage tank, a second liquid storage tank, a second metering pump and a positive pressure source, the second metering pump is connected to the second liquid storage tank and the first liquid storage tank through a pipeline, and the positive pressure source is connected to the second liquid storage tank through a pipeline; the second metering pump is used to provide positive pressure to make the liquid in the second metering pump flow into the first liquid storage tank, and the second metering pump is used to provide negative pressure to draw liquid from the second liquid storage tank to establish negative pressure in the second liquid storage tank, the positive pressure source is used to provide positive pressure to the second liquid storage tank to establish positive pressure in the second liquid storage tank, and the second liquid storage tank is used to provide positive pressure and negative pressure to the liquid containing cavity.

[0015] To solve the above problems, the present application provides a bubble discharge method, which is applied to the above-mentioned blood cell analyzer, and the bubble discharge method includes: when there is a first liquid in the liquid containing cavity, the pressure source cooperates with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure to the liquid containing cavity, so as to fill the liquid in the liquid containing cavity to obtain a second liquid, wherein the bubbles in the second liquid are less than the bubbles in the first liquid.

[0016] Compared with the prior art, the blood cell analyzer of the present application includes: a flow chamber, a pressure source, a first control valve, a second control valve and a third control valve, the flow chamber includes a liquid containing cavity and a first liquid inlet, a second liquid inlet and a liquid outlet connected to the liquid containing cavity, the first control valve is connected to the first liquid inlet through a pipeline, the second control valve is connected to the liquid outlet through a pipeline, the third control valve is connected to the second liquid inlet through a pipeline, and the pressure source is connected to at least one of the first liquid inlet and the second liquid inlet through a pipeline; when there is a first liquid in the liquid containing cavity, the pressure source cooperates with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure to the liquid containing cavity to fill the liquid in the liquid containing cavity to obtain a second liquid, wherein the bubbles in the second liquid are less than the bubbles in the first liquid. Through the above-mentioned implementation mode, the pressure source cooperates with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure to the liquid containing cavity, so as to form a second liquid with fewer bubbles than the first liquid, so that the bubbles in the liquid in the flow chamber can be discharged through a simple structure, the accumulation of bubbles inside the flow chamber can be reduced, and the accuracy and reliability of the detection of the blood cell analyzer can be enhanced. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 is a structural schematic diagram of a first embodiment of a blood cell analyzer provided by the present application;

[0019] Figure 2 is a structural schematic diagram of a second embodiment of a blood cell analyzer provided by the present application;

[0020] Figure 3 is a structural schematic diagram of a third embodiment of a blood cell analyzer provided by the present application;

[0021] Figure 4 Schematic diagram of an embodiment of a flow chamber provided in the present application.

[0022] Figure numbers: blood cell analyzer 10; flow chamber 100; first liquid inlet 110; liquid outlet 120; second liquid inlet 130; liquid containing cavity 140; first cavity 141; second cavity 142; rectifying component 150; rectifying hole 151; sheath liquid cavity 160; first control valve 210; second control valve 220; third control valve 230; sample needle 300; pressure source 400; first metering pump 410; syringe 420; first liquid storage tank 430; second liquid storage tank 440; second metering pump 450; controller 500; first pipeline 610; flow limiting tube 620; reaction pool 630; flow direction X. DETAILED DESCRIPTION

[0023] The following embodiments of the technical solution of the present application are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" in the specification and claims of this application and the above-mentioned figure descriptions and any variations thereof are intended to cover non-exclusive inclusions.

[0025] In the description of the embodiments of the present application, the technical terms "first", "second", etc. are only used to distinguish different objects, and cannot be understood as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the present application, the meaning of "multiple" is more than two, unless otherwise clearly and specifically defined.

[0026] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0027] In the description of the embodiments of the present application, the term "and / or" is only a description of the association relationship of the associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0028] In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two). Similarly, "multiple groups" refers to more than two groups (including two groups), and "multiple pieces" refers to more than two pieces (including two pieces).

[0029] In the description of the embodiments of the present application, the technical terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the embodiments of the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the embodiments of the present application.

[0030] In the description of the embodiments of the present application, unless otherwise clearly specified and limited, technical terms such as "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0031] The present application provides a blood cell analyzer.

[0032] The blood cell analyzer includes a sampling module, a sample preparation module and a flow chamber assembly. The sampling module is used to obtain samples, and the sample preparation module is used to receive samples and mix the samples with reagents to obtain samples to be tested. Among them, the sample preparation module can also include a transportation module, a reagent distribution module, a reagent module and a reaction module. The flow chamber assembly includes a flow chamber and an optical detection device. When in use, the original sample is transferred to the sampling module through the transportation module, and the sampling of the sampling module is used to quantitatively sample the original sample, and then transferred to the reaction module to wait for reaction. The reagent module is used to accommodate and prepare the reagents required for sample testing, and the transportation module and the reagent distribution module quantitatively add the reagents currently needed to be added to the sample to the reaction module.

[0033] The added reagents and the original sample are mixed in the reaction module to form a sample to be tested. The sample to be tested enters the flow chamber assembly. When the sample particles of the sample to be tested pass through the detection flow channel of the flow chamber assembly, the optical detection device detects the sample to be tested and converts the photoelectric data to obtain the detection data.

[0034] However, the flow chamber is a key component in the blood cell analyzer, which is mainly used to form a stable sample flow for detection. During the use of the flow chamber, it is necessary to keep the inside free of bubbles as much as possible, because the bubbles accumulated inside the flow chamber may cause the following effects: (1) The sample flow is not stable enough. The presence of bubbles will affect the pressure balance of the flow channel, causing the sample flow to jitter, or even unable to form a sample flow, affecting the performance of the instrument. (2) The bubbles themselves are identified as particles, making the test results inaccurate. (3) The bubble signal overwhelms the cell signal, resulting in a low test result. In order to improve the accuracy of sample detection, it is necessary to keep the flow chamber as free of bubbles as possible. Some common ways to remove bubbles are to flush away the bubbles by increasing the flow rate.

[0035] However, the flow channel in the detection area at the top of the flow chamber is narrow, while the steady flow area at the bottom is larger. Due to the flow channel restriction in the detection area, it is difficult to increase the liquid flow rate through the flow chamber, and the flow in the steady flow area inside the flow chamber is usually relatively stable with a low flow rate. The effect of discharging bubbles by increasing the flow rate is not obvious, and ultimately small bubbles are easily adhered to the inner wall of the flow chamber, and large bubbles are easily blocked in a certain flow channel and cannot be discharged.

[0036] Based on the above technical problems, the present application provides a blood cell analyzer, see Figure 1 , Figure 1 It is a structural schematic diagram of the first embodiment of the blood cell analyzer provided by the present application.

[0037] The blood cell analyzer 10 includes a flow chamber 100. The flow chamber 100 includes a liquid containing cavity 140 and a first liquid inlet 110, a second liquid inlet 130 and a liquid outlet 120 connected to the liquid containing cavity 140. The first liquid inlet 110 may be a sheath liquid inlet. The first liquid inlet 110 is used to connect a sheath liquid pool through a pipeline. The sheath liquid may enter the liquid containing cavity 140 from the first liquid inlet 110 to form a sheath flow in the liquid containing cavity 140. The sample to be tested may enter the liquid containing cavity 140 from the second liquid inlet 130. After the sample particles (e.g., blood cells) of the sample to be tested entering the liquid containing cavity 140 are wrapped by the sheath liquid, they pass through the detection channel of the flow chamber 100 one by one under the pressure, thereby detecting the sample particles.

[0038] The blood cell analyzer 10 includes a pressure source 400, a first control valve 210, a second control valve 220 and a third control valve 230. The first control valve 210 is connected to the first liquid inlet 110 through a pipeline, the second control valve 220 is connected to the liquid outlet 120 through a pipeline, and the third control valve 230 is connected to the second liquid inlet 130 through a pipeline. The pressure source 400 is connected to at least one of the first liquid inlet 110 and the second liquid inlet 130 through a pipeline. When the first liquid is present in the liquid containing cavity 140, the pressure source 400 cooperates with the first control valve 210, the second control valve 220 and the third control valve 230 to provide positive pressure and negative pressure to the liquid containing cavity 140, so as to fill the liquid in the liquid containing cavity 140 with liquid to obtain a second liquid, wherein the bubbles in the second liquid are less than the bubbles in the first liquid.

[0039] The first control valve 210, the second control valve 220 and the third control valve 230 are all controllable valves, such as being able to switch between two states of on or off by receiving a control signal. The pressure source 400 can provide positive pressure and negative pressure. In some embodiments, the first control valve 210 can be turned on or off, the second control valve 220 can be turned on or off, and the third control valve 230 can be turned on or off, and then the pressure source 400 can be used to provide positive pressure and negative pressure to the liquid-containing cavity 140, thereby forming a second liquid with fewer bubbles than the first liquid.

[0040] The first liquid in the liquid containing cavity 140 can be considered as a liquid that has not been treated to discharge bubbles. For example, when the liquid containing cavity 140 does not contain liquid, the liquid enters the liquid containing cavity 140 through the first liquid inlet 110, thereby forming the first liquid in the liquid containing cavity 140. The second liquid can be considered as a liquid that has been treated to discharge bubbles. Therefore, the bubbles retained in the second liquid should be less than the bubbles retained in the first liquid. It should be noted that the bubbles in the second liquid here are less than the bubbles in the first liquid, which can be understood as: when the two are compared at the same volume, such as when the first liquid and the second liquid both fill the liquid containing cavity 140, the bubbles in the second liquid are less than the bubbles in the first liquid. Or the bubbles in the second liquid are less than the bubbles in the first liquid, which can also be understood as: the ratio of the number of bubbles in the unit volume of the two, the bubbles in the second liquid are less than the bubbles in the first liquid. Or the bubbles in the second liquid are less than the bubbles in the first liquid, which can also be understood as: when the first liquid and the second liquid are at the same volume, the total volume and / or the number of bubbles contained in the second liquid are less than the total volume and / or the number of bubbles contained in the first liquid.

[0041] Through the above-mentioned implementation, the pressure source 400 cooperates with the first control valve 210, the second control valve 220 and the third control valve 230 to provide positive pressure and negative pressure to the liquid containing cavity 140, so as to form a second liquid with fewer bubbles than the first liquid, so that the bubbles in the liquid in the flow chamber 100 can be discharged through a simple structure, the accumulation of bubbles inside the flow chamber 100 can be reduced, and the accuracy and reliability of the detection of the blood cell analyzer 10 can be enhanced.

[0042] See also Figure 2 , Figure 2 It is a structural schematic diagram of the second embodiment of the blood cell analyzer 10 provided in the present application.

[0043] The pressure source 400 is connected to at least the second liquid inlet 130 through a pipeline, and the liquid containing cavity 140, the pipeline connecting the flow chamber 100 and the first control valve 210, the pipeline connecting the flow chamber 100 and the second control valve 220, and the pipeline connecting the pressure source 400 and the second liquid inlet 130 are all filled with the first liquid.

[0044] When the first control valve 210 and the second control valve 220 are set to the cut-off state, and the third control valve 230 is set to the on state, the pressure source 400 provides negative pressure to the liquid containing cavity 140, so that at least part of the bubbles in the liquid containing cavity 140 become larger. Since the liquid containing cavity 140, the pipeline connecting the flow chamber 100 and the first control valve 210, the pipeline connecting the flow chamber 100 and the second control valve 220, and the pipeline connecting the pressure source 400 and the second liquid inlet 130 are all filled with the first liquid, when the first control valve 210 and the second control valve 220 are set to the cut-off state, and the third control valve 230 is set to the on state, the flow chamber 100 is in a completely closed state, and when the pressure source 400 provides negative pressure to the liquid containing cavity 140 through the second liquid inlet 130, the liquid in the liquid containing cavity 140 will not move significantly. In one embodiment, when the first control valve 210 and the second control valve 220 are set to the cut-off state and the third control valve 230 is set to the on state, negative pressure can be provided by the pressure source 400 connected to the third control valve 230. In another embodiment, the pressure source includes a syringe connected to the second liquid inlet 130, and a reaction pool is further connected to the connecting pipeline between the syringe and the second liquid inlet 130. When the first control valve 210 and the second control valve 220 are set to the cut-off state and the third control valve 230 is set to the on state, negative pressure can be provided by the above-mentioned syringe. In other embodiments, the pressure source includes a pressure source (such as a metering pump) connected to the third control valve 230 and a syringe connected to the second liquid inlet 130, and a reaction pool is further connected to the connecting pipeline between the syringe and the second liquid inlet 130. When the first control valve 210 and the second control valve 220 are set to the cut-off state and the third control valve 230 is set to the on state, negative pressure can be provided by the above-mentioned pressure source connected to the third control valve 230 and the above-mentioned syringe together.

[0045] In some specific scenarios, when there are bubbles inside the liquid-containing cavity 140, small bubbles may adhere to the wall of the liquid-containing cavity 140. Due to the buoyancy, fluid drag, and shear force of the bubbles themselves, it is difficult to break this equilibrium state and expel or break the bubbles; while large bubbles may block some narrow flow channels. At this time, it is difficult to expel the bubbles even if the liquid is flushed. In this embodiment, when the liquid-containing cavity 140 is in a closed state, negative pressure is provided to the liquid-containing cavity 140, and the negative pressure is transmitted to the bubble surface inside the flow chamber 100 through the fluid, so that the bubbles swell or even break. For small bubbles, the external pressure decreases, the bubble diameter increases, and the buoyancy, fluid drag, and fluid shear force it is subjected to increase with the increase of the bubble diameter, so that the bubbles are easier to detach from the wall; due to the increase in the bubble diameter, the external action is more likely to cause the bubbles to deform or even break, and the large bubbles are broken and transformed into small bubbles, which are easier to pass through narrow channels.

[0046] After the pressure source 400 provides negative pressure to the liquid containing cavity 140, the first control valve 210 and the second control valve 220 can be set to the conducting state, and the third control valve 230 can be set to the cut-off state, and the pressure source 400 can provide positive pressure to the liquid containing cavity 140, and inject liquid into the liquid containing cavity 140 at least through the second liquid inlet 130, so that at least part of the enlarged bubbles are broken and discharged from the liquid containing cavity 140, and then the liquid containing cavity 140 is filled with the second liquid. Due to the rapid change in the internal pressure of the liquid containing cavity 140, the enlarged bubbles are broken, and the pressure source 400 provides positive pressure to the liquid containing cavity 140, which can discharge the broken bubbles from the liquid outlet 120, so that the liquid containing cavity 140 is filled with the second liquid, and the bubbles accumulated in the liquid containing cavity 140 are reduced. The first liquid discharged from the liquid containing cavity 140 may be discharged into a solution pool, and after the first liquid is left to stand in the solution pool for a period of time, bubbles in the first liquid may float to the surface of the liquid, or bubbles may separate from the first liquid; or the first liquid discharged from the liquid containing cavity 140 may be discharged into the solution pool first, and then the first liquid in the solution pool may be discharged into the waste liquid pool to replace the liquid in the solution pool; or the first liquid discharged from the liquid containing cavity 140 may be directly discharged into the waste liquid pool. In one embodiment, the pressure source 400 includes a positive air pressure source connected to the second liquid inlet 130, and positive pressure may be provided to the liquid containing cavity 140 through the positive air pressure source. In another embodiment, the pressure source 400 includes a positive air pressure source connected to the second liquid inlet 130, and the positive air pressure source and the second liquid inlet 130 are connected through a parallel limiting tube A and a pipeline B, and the liquid flow rate in the limiting tube A is slower than the liquid flow rate in the pipeline B; preferably, positive pressure may be provided to the liquid containing cavity 140 through the positive air pressure source and the pipeline B. By using a larger positive pressure, that is, a positive pressure with a larger pressure difference compared to the previous negative pressure, the rapid switching of negative pressure to positive pressure can be effectively enhanced, and the pressure inside the flow chamber changes dramatically from negative pressure to a larger positive pressure (relative to turning on the positive pressure source and the flow limiting tube A), so that the bubbles in the flow chamber are broken and taken away.

[0047] Further, the blood cell analyzer 10 further includes: a controller 500, which is used to: control the repeated execution according to the preset execution times: when the first control valve 210 and the second control valve 220 are set to the cut-off state, and the third control valve 230 is set to the on state, the pressure source 400 provides negative pressure to the liquid containing cavity 140, so that at least part of the bubbles in the liquid containing cavity 140 become larger; when the first control valve 210 and the second control valve 220 are set to the on state, and the third control valve 230 is set to the cut-off state, the pressure source 400 provides positive pressure to the liquid containing cavity 140, and injects liquid into the liquid containing cavity 140 at least through the second liquid inlet 130, so that at least part of the enlarged bubbles are broken and discharged from the liquid containing cavity 140. The controller 500 can be the overall control system of the blood cell analyzer 10, and the preset execution times can be two, three, four or other times. In order to obtain the second liquid with less gas content as much as possible, it can be achieved by repeatedly executing the positive and negative pressure alternation method for multiple times.

[0048] See also Figure 3 , Figure 3 It is a structural schematic diagram of the third embodiment of the blood cell analyzer 10 provided in the present application.

[0049] The pressure source 400 is connected to the first liquid inlet 110 and the second liquid inlet 130 through a pipeline.

[0050] When the first liquid is present in the liquid containing cavity 140, when the third control valve 230 and the first control valve 210 are set to a conducting state, and the second control valve 220 is set to a cut-off state, the pressure source 400 provides negative pressure to the liquid containing cavity 140, so that the liquid in the liquid containing cavity 140 flows out of the liquid containing cavity 140 from the first liquid inlet 110 at a first flow rate less than a preset flow rate threshold. When the first liquid is present in the liquid containing cavity 140, since the gas content of the first liquid does not meet the requirements, it is necessary to perform a bubble removal process on the liquid in the liquid containing cavity 140. In the present embodiment, this is achieved by first emptying the first liquid in the liquid containing cavity 140 and then filling the second liquid into the liquid containing cavity 140. Specifically, a predetermined negative pressure is provided to the liquid containing cavity 140 through the pressure source 400, so that the first liquid flows out of the liquid containing cavity 140 at a relatively small flow rate, thereby achieving the effect of evacuating the liquid containing cavity 140. Since the first liquid flows out of the liquid containing cavity 140 at a relatively small flow rate, the problem of bubbles remaining inside the liquid containing cavity 140 can also be reduced.

[0051] When the first control valve 210 and the second control valve 220 are set to the conducting state, and the third control valve 230 is set to the shutoff state, the pressure source 400 provides positive pressure to the liquid containing cavity 140, so that the liquid flows into the liquid containing cavity 140 from the first liquid inlet 110 at a second flow rate less than the preset flow rate threshold through the pressure source 400, so as to fill the liquid containing cavity 140 with the liquid. After the liquid containing cavity 140 is evacuated, the liquid containing cavity 140 needs to be filled with liquid again. However, in normal use scenarios or during sheath liquid filling (flow chamber 100 from empty to full), bubbles may enter the flow chamber 100 from the sheath liquid inlet and get stuck in the dead zone of the liquid containing cavity 140. Excessive accumulation of bubbles may affect the width and stability of the sample flow, and ultimately lead to abnormal results. In this embodiment, a predetermined positive pressure is provided to the liquid containing cavity 140 by the pressure source 400, so that the liquid flows out of the liquid containing cavity 140 at a lower flow rate, and the problem of bubbles generated in the liquid containing cavity 140 can be alleviated during the filling process of the sheath liquid. Thus, the second liquid having a smaller bubble content than the first liquid is filled in the liquid containing cavity 140.

[0052] Further, see Figure 3 and Figure 4 , Figure 4 is a schematic diagram of an embodiment of a flow chamber 100 provided in the present application.

[0053] The blood cell analyzer 10 further includes a sample needle 300, a flow limiting tube 620, a first pipeline 610 and a reaction pool 630 connected to atmospheric pressure. The sample needle 300 is located in the liquid containing cavity 140 and connected to the second liquid inlet 130; the reaction pool 630 is connected between the pressure source 400 and the flow chamber 100; the pressure source 400 is at least connected to the first liquid inlet 110, and the pressure source 400 and the first liquid inlet 110 are connected through the flow limiting tube 620 and the first pipeline 610. The first pipeline 610 and the flow limiting tube 620 can be connected to the first liquid inlet 110 in parallel, and the branch where the first pipeline 610 is located and the branch where the flow limiting tube 620 is located can be connected to a control valve, and the pressure source 400 is connected to the first liquid inlet 110 through the first pipeline 610 and / or the flow limiting tube 620 by turning on or off the control valve. The reaction pool 630 can be used to react the original sample with the reagent to obtain the sample to be tested. The reaction pool 630 is connected to the atmospheric pressure and is connected between the pressure source 400 and the flow chamber 100, so that the liquid containing cavity 140 can be connected to the atmospheric pressure through the reaction pool 630. The sample to be tested can enter the sample needle 300 from the second liquid inlet 130, so that the sample to be tested can be transported to a predetermined position through the sample needle 300.

[0054] When the third control valve 230 and the flow limiting tube 620 are set to the conducting state, and the second control valve 220 and the first pipeline 610 are set to the shutoff state, the pressure source 400 provides negative pressure to the liquid containing cavity 140 through the flow limiting tube 620, so that the liquid in the liquid containing cavity 140 flows out of the liquid containing cavity 140 from the first liquid inlet 110 through the flow limiting tube 620 at a first flow rate less than a preset flow rate threshold. The reaction pool 630 can be connected to the second liquid inlet 130 through the third control valve 230. When the first liquid is present in the liquid containing cavity 140, since the gas content of the first liquid does not meet the requirements, it is necessary to perform a bubble removal process on the liquid in the liquid containing cavity 140. In this embodiment, by conducting the third control valve 230, the flow chamber 100 is connected to the atmospheric pressure, so that negative pressure is provided to the liquid containing cavity 140 to extract the first liquid in the liquid containing cavity 140. At the same time, in order to control the flow rate of the first liquid, the flow limiting tube 620 is turned on so that the pressure source 400 is connected to the liquid containing cavity 140 through the flow limiting tube 620, so that the liquid in the liquid containing cavity 140 flows out of the liquid containing cavity 140 from the first liquid inlet 110 through the flow limiting tube 620 at a first flow rate less than a preset flow rate threshold, thereby reducing the problem of bubbles remaining in the liquid containing cavity 140. In addition, since the internal channel of the sample needle is a narrow and long channel, the sample needle plays a role in limiting the flow of the liquid flowing therethrough. Through the coordinated arrangement of the liquid pipeline and the control valve and the structural arrangement of the internal channel of the sample needle, the liquid in the flow chamber can be slowly evacuated through negative pressure, thereby allowing bubbles in the dead zone and other positions in the flow chamber to be discharged from the flow chamber along with the liquid.

[0055] Further, the sample needle 300 is extended along the flow direction X of the liquid in the liquid-containing cavity 140, and the flow chamber 100 also includes a rectifying component 150 disposed in the liquid-containing cavity 140. The rectifying component 150 divides the area in the liquid-containing cavity 140 except the sample needle 300 into a first cavity 141 and a second cavity 142 in the flow direction X of the liquid. The first liquid inlet 110 is connected to the first cavity 141, and the rectifying component 150 is provided with a rectifying hole 151 connecting the first cavity 141 and the second cavity 142. The number of rectifying holes 151 can be set according to actual conditions, such as the number of rectifying holes 151 is 2-8. For example, the number of rectifying holes 151 can include 4, 5, 6 or other numbers. The length of the rectifying hole 151 can be set according to actual conditions. By limiting the length and number of the rectifying holes 151, the liquid can enter the second cavity 142 through the rectifying hole 151 while reducing the possible attachment area of ​​the bubble. The sample needle 300 can be partially inserted into the rectifying component 150, so that one end of the sample needle 300 is connected to the second liquid inlet 130, and the other end extends into a predetermined position of the liquid containing cavity 140. In one embodiment, when the third control valve and the flow limiting tube are set to a conducting state, and the second control valve and the first pipeline are set to a cut-off state, the pressure source provides negative pressure to the liquid containing cavity through the flow limiting tube, so that the first liquid in the first cavity, the second cavity and the rectifying hole flows out of the liquid containing cavity from the first liquid inlet through the flow limiting tube at a first flow rate less than a preset flow rate threshold, thereby at least discharging the bubbles in the first cavity and the rectifying hole; wherein the rectifying hole 151 is set It is a strip-shaped hole. The axial direction of the rectifying hole 151 is parallel to the length direction of the sample needle 300, that is, the axial direction of the rectifying hole 151 is parallel to the extension direction of the sample needle 300. The diameter of the cross section of the rectifying hole 151 is smaller than the distance from the first point to the second point on the reference cross section. The reference cross section is a plane parallel to the cross section of the rectifying hole 151. The first point is a point on the reference cross section where the rectifying component 150 contacts the outer wall of the flow chamber 100. The second point is a point on the reference cross section where the rectifying component 150 contacts the inner wall of the sample needle 300. The rectifying hole 151 is a narrow and elongated flow channel, and can also make the turbulent liquid flow gradually orderly through the rectifying hole 151 and enter the second cavity 142.In order to control the flow rate of the first liquid, the flow limiting tube 620 is turned on so that the pressure source 400 is connected to the liquid containing cavity 140 through the flow limiting tube 620, so that the liquid in the liquid containing cavity 140 flows out of the liquid containing cavity 140 from the first liquid inlet 110 through the flow limiting tube 620 at a first flow rate that is less than a preset flow rate threshold. In addition, due to the larger bubbles located in the second cavity and completely blocking the rectifying holes, the liquid in the second cavity squeezes the larger bubbles under the action of negative pressure, so that the larger bubbles cannot maintain their original shape due to the surface tension of the bubbles caused by the direct negative pressure and the pressure of the liquid in the second cavity, so that the bubbles swell, deform, or even break, and the flow rate increases. and no longer blocks the rectifying hole; or for the bubbles located in the second cavity and partially blocking the rectifying hole, under the action of negative pressure, the surface tension of the bubbles that partially block the rectifying hole cannot maintain the original shape of the bubbles, causing the bubbles to swell, deform or even break; or for the bubbles located in the channel of the rectifying hole, especially for the bubbles that block the channel of the rectifying hole, under the action of negative pressure, the bubbles in the narrow channel are swelled under the action of external pressure, and due to the limitation of the inner wall of the channel, the surface tension inside the bubbles cannot maintain the original shape of the bubbles, making the bubbles more likely to deform, and then break into smaller bubbles that are not easy to block the rectifying hole, and then the bubbles that are easy to block the rectifying hole are discharged. In addition, the dead zone in the first cavity is located in the area of ​​the second cavity close to the rectifying component. The dead zone is prone to unstable flow of liquid in the first cavity due to large liquid disturbance when filling the first cavity with liquid, resulting in part of the dead zone of the first cavity being unable to be filled, and bubbles forming in part of the dead zone. Through the action of negative pressure, large bubbles in the dead zone are deformed or even broken, and small bubbles increase in diameter due to external force and then detach from the wall or break, so as to discharge the bubbles in the dead zone.

[0056] When the pressure source 400 causes the liquid to flow from the first liquid inlet 110 into the liquid containing cavity 140 at a second flow rate, if it is detected that the liquid enters the second cavity 142 through the rectifying hole 151, the second control valve 220 is set to a conducting state, and the liquid is caused to flow from the first liquid inlet 110 into the first cavity 141 at a fourth flow rate through the pressure source 400, so that the liquid fills the first cavity 141 and the second cavity 142; wherein the fourth flow rate is greater than the second flow rate. If liquid needs to fill the liquid containing cavity 140, it needs to first enter the first cavity 141 from the first liquid inlet 110. At this time, the liquid can enter the first cavity 141 at the second flow rate and in a direction perpendicular to the flow direction X. Since the liquid enters the first cavity 141 at the second flow rate, it can alleviate the situation where a large number of bubbles are generated due to the collision between the liquid and the inner wall of the liquid containing cavity 140; and since the liquid enters the liquid containing cavity 140 from the first liquid inlet 110 at the second flow rate, the second control valve 140 is in a cut-off state at this time, and the liquid containing cavity 140 is relatively in a pressure-holding state. At this time, the second flow rate is a relatively slow flow rate. After the first cavity 141 is filled, the liquid will gradually fill the rectifying hole 151, and then gradually enter the second cavity 142 along the rectifying hole 151. When it is detected that the liquid enters the second cavity 142 through the rectifying hole 151, the second control valve 220 can be set to a conducting state, so that the pressure-blocking state of the liquid-containing cavity 140 is released. When the same positive pressure is output, the flow rate of the liquid in the liquid-containing cavity 140 can be increased, and the second flow rate can be increased to the fourth flow rate, thereby speeding up the filling of the liquid-containing cavity 140 with liquid and improving the overall working efficiency.

[0057] Further, the liquid containing cavity 140 includes a sheath liquid cavity 160 wrapped around the outside of the sample needle 300. The liquid containing cavity 140 can be divided into the sheath liquid cavity 160 for the sheath liquid to flow and the accommodating cavity for accommodating the sample needle 300. When the liquid fills the sheath liquid cavity 160, the pressure source 400 provides positive pressure to the liquid containing cavity 140, so that the liquid flows from the first liquid inlet 110 into the liquid containing cavity 140 at a third flow rate through the pressure source 400, wherein the third flow rate is greater than the second flow rate, and / or the third flow rate is greater than the fourth flow rate; when the liquid is at the third flow rate, the pressure source 400 injects the liquid into the sample needle 300 to fill the sample needle 300 with the liquid. In one embodiment, the pressure source 400 injects the sample into the sample needle 300 at a fifth flow rate to transport the sample through the sample needle, so that the sheath liquid flowing in the sheath liquid cavity wraps the sample to form a sample flow in the flow chamber, and then the sample is detected in the flow chamber, wherein the third flow rate is greater than the fifth flow rate.

[0058] Since the sheath liquid cavity 160 has been filled with liquid, and in order to quickly put the flow chamber 100 into a ready-to-use state, it is necessary to inject liquid into the sample needle 300. In this embodiment, when the sheath liquid cavity 160 is filled with liquid, the liquid is made to flow into the sheath liquid cavity 160 at a third flow rate to quickly establish a sheath flow with a relatively high flow rate in the sheath liquid cavity 160. In the process of injecting liquid into the sample needle 300 to fill the sample needle 300, even if some bubbles enter the sheath liquid cavity 160 from the outlet of the sample needle 300 (the end opening of the sample needle 300 away from the second liquid inlet 130), the sample carrying the bubbles can be wrapped by the sheath flow with a relatively high flow rate or the bubbles can be taken out of the flow chamber from the liquid outlet 120. In particular, the sample carrying the bubbles or the bubbles can be wrapped by the sheath flow with a relatively high flow rate and pass through the detection area of ​​the flow chamber, thereby reducing the contact between the sample carrying the bubbles or the bubbles and the inner wall of the flow chamber, thereby completing the purpose of evacuating bubbles from the flow chamber 100, and further reducing the problem of bubbles remaining inside the liquid containing cavity 140.

[0059] Further, when the liquid fills the sheath liquid cavity 160, the second control valve 220, the flow limiting tube 620 and the first pipeline 610 are all set to a conducting state, and the pressure source 400 provides positive pressure to the liquid containing cavity 140 through the flow limiting tube 620, the first pipeline 610 and the second control valve 220, so that the liquid flows into the liquid containing cavity 140 from the first liquid inlet 110 at a third flow rate; and when the liquid is at the third flow rate, the third control valve 230 is set to a conducting state, and the pressure source 400 injects liquid into the sample needle 300 to fill the sample needle 300 with the liquid.

[0060] The first pipeline 610 and the flow limiting tube 620 can be connected to the first liquid inlet 110 in parallel, and the branch where the first pipeline 610 is located and the branch where the flow limiting tube 620 is located can be connected to a control valve, and the pressure source 400 is connected to the first liquid inlet 110 through the first pipeline 610 and / or the flow limiting tube 620 by turning on or off the control valve. In order to allow the liquid to enter the sheath liquid cavity 160 at a higher flow rate when the liquid fills the sheath liquid cavity 160, the second control valve 220, the flow limiting tube 620 and the first pipeline 610 can be set to a conducting state, so that the liquid can enter the first liquid inlet 110 through the first pipeline 610 and the flow limiting tube 620 at the same time. When the pressure source 400 provides positive pressure to the liquid containing cavity 140, more liquid can flow into the liquid containing cavity 140 per unit time, so that the liquid flows from the first liquid inlet 110 into the liquid containing cavity 140 at a third flow rate. During this process, the pressure source 400 injects liquid into the sample needle 300 to fill the sample needle 300 with the liquid, and the bubbles can be brought out of the flow chamber 100 from the liquid outlet 120 through the sheath flow with a higher flow rate, thereby completing the purpose of exhausting bubbles from the flow chamber 100, thereby reducing the problem of bubbles remaining inside the liquid containing cavity 140.

[0061] In the present application, during the process of evacuating the liquid in the flow chamber at a first flow rate, the evacuation process is slow due to the flow limitation of the sample needle, and the bubbles on the inner wall or dead zone of the flow chamber cannot maintain their shape and break due to the effect of surface tension when they come into contact with the air; in the subsequent process of filling the liquid at at least a second flow rate, when the sheath liquid cavity of the flow chamber is not yet filled with liquid, the liquid flow rate is low (the second flow rate is relatively low) to avoid excessive liquid disturbance, which may cause unstable flow of the liquid inside the flow chamber, resulting in some dead zones being unable to be filled and some gas being retained in the dead zones; during the final filling of the sample needle, under the protection of the sheath liquid, the air inside the sample needle is confined in the sample flow and cannot come into contact with the internal structure of the flow chamber, and the bubbles can be smoothly discharged from the flow chamber under the wrapping of the sheath liquid, thereby achieving the effect of evacuating bubbles from the flow chamber.

[0062] See also Figure 2 and Figure 3 The pressure source 400 includes a first metering pump 410, which is connected to the second liquid inlet 130 through a pipeline. The first metering pump 410 is used to provide positive pressure and negative pressure to the liquid containing cavity 140. One end of the first metering pump 410 can be connected to a positive air pressure source and a negative air pressure source through a pipeline, and the other end can be connected to the second liquid inlet 130 through a pipeline. When it is necessary to provide positive pressure to the liquid containing cavity 140, positive pressure can be output to the first metering pump 410 through the positive air pressure source to provide positive pressure to the liquid containing cavity 140; when it is necessary to provide negative pressure to the liquid containing cavity 140, negative pressure can be output to the first metering pump 410 through the negative air pressure source to provide negative pressure to the liquid containing cavity 140.

[0063] The pressure source 400 includes a syringe 420, which is connected to the second liquid inlet 130 through a pipeline, and the syringe 420 is used to provide positive pressure and negative pressure to the liquid containing cavity 140. In this embodiment, the first metering pump 410 and the syringe 420 can be connected to the second liquid inlet 130 at the same time, and the syringe 420 or the first metering pump 410 can be controlled to provide positive pressure or negative pressure to the liquid containing cavity 140 respectively, or the syringe 420 and the first metering pump 410 can be controlled to provide positive pressure or negative pressure to the liquid containing cavity 140 at the same time.

[0064] In some embodiments, the pressure source 400 includes a first liquid storage tank 430, a second liquid storage tank 440, a second metering pump 450 and a positive pressure source, the second metering pump 450 is connected to the second liquid storage tank 440 and the first liquid storage tank 430 through a pipeline, and the positive pressure source is connected to the second liquid storage tank 440 through a pipeline; the second metering pump 450 is used to provide positive pressure to allow the liquid in the second metering pump 450 to flow into the first liquid storage tank 430, and the second metering pump 450 is used to provide negative pressure to draw liquid from the second liquid storage tank 440 to establish negative pressure in the second liquid storage tank 440, the positive pressure source is used to provide positive pressure to the second liquid storage tank 440 to establish positive pressure in the second liquid storage tank 440, and the second liquid storage tank 440 is used to provide positive and negative pressure to the liquid containing cavity 140.

[0065] Liquid is stored in both the first liquid storage tank 430 and the second liquid storage tank 440, and a positive pressure or a negative pressure can be established in the second liquid storage tank 440, and then the positive pressure or the negative pressure is provided to the liquid containing cavity 140 through the second liquid storage tank 440. Specifically, when it is necessary to establish a negative pressure in the second liquid storage tank 440, the second liquid storage tank 440 is first placed in a closed state, and then the liquid in the second metering pump 450 is transferred to the first liquid storage tank 430 through a pipeline, and then the liquid is sucked from the second liquid storage tank 440 through the second metering pump 450 to form a certain negative pressure in the second liquid storage tank 440. When a greater negative pressure needs to be established in the second liquid storage tank 440, it can be achieved by repeating the above method. When it is necessary to establish a positive pressure in the second liquid storage tank 440, the second liquid storage tank 440 is first placed in a closed state, and then a positive pressure is input to the second liquid storage tank 440 through a positive pressure source, so as to form a predetermined positive pressure in the second liquid storage tank 440.

[0066] Combination Figure 3The second liquid storage tank 440 may store a diluent, which may also be used as a sheath fluid during the sample detection process. The second liquid storage tank 440 is connected to the first control valve 210 through a pipeline. The second liquid storage tank 440 may be connected to atmospheric pressure through a control valve. In one embodiment, the control valve connected to atmospheric pressure may be opened to restore the second liquid storage tank 440 to normal pressure. After normal pressure is restored, the connection between the second liquid storage tank 440 and atmospheric pressure is disconnected, and the second liquid storage tank 440 is in a sealed state. The diluent in the second liquid storage tank 440 is sucked into the second metering pump 450 by the second metering pump 450, and the second metering pump 450 pumps the diluent sucked therein into the first liquid storage tank 430, and then the diluent in the second liquid storage tank 440 is sucked into the second metering pump 450 by the second metering pump, and the second metering pump 450 pumps the diluent sucked therein into the first liquid storage tank 430; this is repeated many times. Since the second liquid storage tank 440 is in a closed state, the diluent in the second liquid storage tank 440 is sucked by the second metering pump 450 many times, and the second liquid storage tank 440 gradually forms a negative pressure from normal pressure. Exemplarily, the negative pressure of the second liquid storage tank 440 can be set to -40KPA at this time. In another embodiment, a positive pressure can be established in the second liquid storage tank 440 by a positive air pressure source connected to another control valve connected to the second liquid storage tank 440. Exemplarily, another control valve connected to the second liquid storage tank 440 is opened to establish a positive pressure in the second liquid storage tank 440, for example, a positive pressure of about 70 KPA.

[0067] In order to solve the technical problems existing in the prior art, the present application also provides a bubble discharge method, which can be applied to the blood cell analyzer 10 of any of the above-mentioned embodiments. The bubble discharge method may include: when the first liquid is present in the liquid containing cavity 140, the pressure source 400 cooperates with the first control valve 210, the second control valve 220 and the third control valve 230 to provide positive pressure and negative pressure to the liquid containing cavity 140 to fill the liquid in the liquid containing cavity 140 to obtain a second liquid, wherein the bubbles in the second liquid are less than the bubbles in the first liquid.

[0068] The bubble discharge method can be implemented based on the various components in the blood cell analyzer 10 of the above embodiment. The specific implementation method can be the same or similar to the methods of the various embodiments in the above blood cell analyzer 10, and will not be repeated here.

[0069] Through the above-mentioned implementation, the pressure source 400 cooperates with the first control valve 210, the second control valve 220 and the third control valve 230 to provide positive pressure and negative pressure to the liquid containing cavity 140, so as to form a second liquid with fewer bubbles than the first liquid, so that the bubbles in the liquid in the flow chamber 100 can be discharged through a simple structure, the accumulation of bubbles inside the flow chamber 100 can be reduced, and the accuracy and reliability of the detection of the blood cell analyzer 10 can be enhanced.

[0070] The above structure and method can be used to remove bubbles from the flow chamber during normal use of the blood cell analyzer, and can also be used to remove bubbles from the flow chamber during the perfusion process of the blood cell analyzer. The perfusion process includes the initial perfusion and the bubble removal process of the blood cell analyzer described in this application. Before the bubble is discharged from the flow chamber, the first control valve, the second control valve, and the third control valve are all turned on, and the flow chamber and the pipelines connected to the liquid outlet, the first liquid inlet, and the second liquid inlet of the flow chamber are first filled with liquid, so that the liquid fills the flow chamber and the front and rear end pipelines connected to the flow chamber with liquid. The above structure and method help to optimize the perfusion process and avoid the introduction of bubbles during the perfusion process.

[0071] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them; although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application, and they should all be included in the scope of the claims and specification of the present application. In particular, as long as there is no structural conflict, the various technical features mentioned in the various embodiments can be combined in any way. The present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions that fall within the scope of the claims.

Claims

1. A blood cell analyzer, characterized in that: The blood cell analyzer comprises: a flow chamber, a pressure source, a first control valve, a second control valve and a third control valve, the flow chamber comprises a liquid containing cavity and a first liquid inlet, a second liquid inlet and a liquid outlet communicated with the liquid containing cavity, the first control valve is connected to the first liquid inlet through a pipeline, the second control valve is connected to the liquid outlet through a pipeline, the third control valve is connected to the second liquid inlet through a pipeline, and the pressure source is connected to at least one of the first liquid inlet and the second liquid inlet through a pipeline; When there is a first liquid in the liquid containing cavity, the pressure source cooperates with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure to the liquid containing cavity to fill the liquid in the liquid containing cavity to obtain a second liquid, wherein the bubbles in the second liquid are less than the bubbles in the first liquid.

2. The blood cell analyzer according to claim 1, characterized in that: The pressure source is connected to at least the second liquid inlet through a pipeline, and the liquid containing cavity, the pipeline connecting the flow chamber and the first control valve, the pipeline connecting the flow chamber and the second control valve, and the pipeline connecting the pressure source and the second liquid inlet are all filled with the first liquid; When the first control valve and the second control valve are set to the cut-off state and the third control valve is set to the on state, the pressure source provides negative pressure to the liquid containing cavity so as to enlarge at least part of the bubbles in the liquid containing cavity; When the first control valve and the second control valve are set to a conducting state and the third control valve is set to a cut-off state, the pressure source provides positive pressure to the liquid containing cavity and injects liquid into the liquid containing cavity through at least the second liquid inlet, so that at least part of the enlarged bubbles are broken and discharged from the liquid containing cavity, thereby filling the liquid containing cavity with the second liquid.

3. The blood cell analyzer according to claim 2, characterized in that: The blood cell analyzer also includes: a controller, which is used to: control the repeated execution according to a preset number of executions: when the first control valve and the second control valve are set to the cut-off state, and the third control valve is set to the on state, the pressure source provides negative pressure to the liquid containing cavity to enlarge at least part of the bubbles in the liquid containing cavity; when the first control valve and the second control valve are set to the on state, and the third control valve is set to the cut-off state, the pressure source provides positive pressure to the liquid containing cavity, and injects liquid into the liquid containing cavity through at least the second liquid inlet, so that at least part of the enlarged bubbles are broken and discharged from the liquid containing cavity.

4. The blood cell analyzer according to claim 1, characterized in that: The pressure source is connected to the first liquid inlet and the second liquid inlet through a pipeline. When the first liquid is in the liquid containing cavity, the third control valve and the first control valve are set to a conducting state, and the second control valve is set to a cut-off state, the pressure source provides negative pressure to the liquid containing cavity, so that the liquid in the liquid containing cavity flows out of the liquid containing cavity from the first liquid inlet at a first flow rate that is less than a preset flow rate threshold; When the first control valve and the second control valve are set to a conducting state, and the third control valve is set to a cut-off state, the pressure source provides positive pressure to the liquid containing cavity, so that the liquid flows from the first liquid inlet into the liquid containing cavity through the pressure source at a second flow rate that is less than the preset flow rate threshold, so as to fill the liquid containing cavity with the liquid.

5. The blood cell analyzer according to claim 4, characterized in that: The blood cell analyzer further comprises a sample needle, a flow limiting tube, a first pipeline and a reaction pool connected to atmospheric pressure, wherein the sample needle is located in the liquid containing cavity and connected to the second liquid inlet; the reaction pool is connected between the pressure source and the flow chamber; the pressure source is connected to at least the first liquid inlet, and the pressure source and the first liquid inlet are connected via the flow limiting tube and the first pipeline; When the third control valve and the flow limiting tube are set to a conducting state, and the second control valve and the first pipeline are set to a cut-off state, the pressure source provides negative pressure to the liquid containing cavity through the flow limiting tube, so that the liquid in the liquid containing cavity flows out of the liquid containing cavity from the first liquid inlet through the flow limiting tube at a first flow rate that is less than a preset flow rate threshold.

6. The blood cell analyzer according to claim 5, characterized in that: The blood cell analyzer further comprises a sample needle, the sample needle is located in the liquid containing cavity and connected to the second liquid inlet, the sample needle is extended along the flow direction of the liquid in the liquid containing cavity, the flow chamber further comprises a rectifying component arranged in the liquid containing cavity, the rectifying component divides the area in the liquid containing cavity except the sample needle into a first cavity and a second cavity in the flow direction of the liquid, the first liquid inlet is connected to the first cavity, and the rectifying component is provided with a rectifying hole connecting the first cavity and the second cavity; When the third control valve and the flow limiting tube are set to a conducting state, and the second control valve and the first pipeline are set to a shut-off state, the pressure source provides negative pressure to the liquid containing cavity through the flow limiting tube, so that the first liquid in the first cavity, the second cavity and the rectifying hole flows out of the liquid containing cavity from the first liquid inlet through the flow limiting tube at a first flow rate less than a preset flow rate threshold, thereby at least discharging the bubbles in the first cavity and the rectifying hole; when the pressure source flows the liquid into the liquid containing cavity from the first liquid inlet at the second flow rate, if it is detected that the liquid enters the second cavity through the rectifying hole, the second control valve is set to a conducting state, and the liquid flows into the first cavity from the first liquid inlet at a fourth flow rate through the pressure source, so that the liquid fills the first cavity and the second cavity; wherein the fourth flow rate is greater than the second flow rate.

7. The blood cell analyzer according to any one of claims 4 to 6, characterized in that: The blood cell analyzer further comprises a sample needle, the sample needle is located in the liquid containing cavity and connected to the second liquid inlet, the sample needle is extended along the flow direction of the liquid in the liquid containing cavity, and the liquid containing cavity comprises a sheath liquid cavity wrapped around the outside of the sample needle; When the sheath liquid cavity is filled with liquid, the pressure source provides positive pressure to the liquid containing cavity, so that the liquid flows from the first liquid inlet into the liquid containing cavity at a third flow rate through the pressure source, wherein the third flow rate is greater than the second flow rate, and / or the third flow rate is greater than the fourth flow rate; While the liquid is at the third flow rate, the pressure source injects the liquid into the sample needle to fill the sample needle with the liquid.

8. The blood cell analyzer according to claim 7, characterized in that: The blood cell analyzer further comprises a flow limiting tube and a first pipeline, and the pressure source and the first liquid inlet are connected via the flow limiting tube and the first pipeline; When the sheath liquid cavity is filled with liquid, the second control valve, the flow limiting tube and the first pipeline are all set to a conducting state, and the pressure source provides positive pressure to the liquid containing cavity through the flow limiting tube, the first pipeline and the second control valve, so that liquid flows into the liquid containing cavity from the first liquid inlet at the third flow rate; And when the liquid is at the third flow rate, the third control valve is set to a conducting state, and the pressure source injects liquid into the sample needle to fill the sample needle with the liquid.

9. The blood cell analyzer according to claim 1, characterized in that: The pressure source comprises a first metering pump, which is connected to the second liquid inlet via a pipeline, and is used to provide positive pressure and negative pressure to the liquid containing cavity, and / or the pressure source comprises a syringe, which is connected to the second liquid inlet via a pipeline, and is used to provide positive pressure and negative pressure to the liquid containing cavity; And / or, the pressure source includes a first liquid storage tank, a second liquid storage tank, a second metering pump and a positive pressure source, the second metering pump is connected to the second liquid storage tank and the first liquid storage tank through a pipeline, and the positive pressure source is connected to the second liquid storage tank through a pipeline; the second metering pump is used to provide positive pressure to allow the liquid in the second metering pump to flow into the first liquid storage tank, and the second metering pump is used to provide negative pressure to draw liquid from the second liquid storage tank to establish negative pressure in the second liquid storage tank, the positive pressure source is used to provide positive pressure to the second liquid storage tank to establish positive pressure in the second liquid storage tank, and the second liquid storage tank is used to provide positive pressure and negative pressure to the liquid containing cavity.

10. A method for emitting bubbles, characterized in that: Applied to the blood cell analyzer according to any one of claims 1 to 9, the bubble discharge method comprises: When there is a first liquid in the liquid containing cavity, the pressure source cooperates with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure to the liquid containing cavity to fill the liquid in the liquid containing cavity to obtain a second liquid, wherein the bubbles in the second liquid are less than the bubbles in the first liquid.

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