Kit and blood cell analyzer

Abstract

The application discloses a kit and a blood cell analyzer. One end of one electrode of the front cell electrode and the rear cell electrode of the kit extends to one side where the other end of the other electrode is exposed on the same side of the shell through a microporous sheet, so that the kit can be connected with the front cell electrode and the rear cell electrode in one direction when the kit is electrically connected with the blood cell analyzer. The alignment process of the kit and the blood cell analyzer is simple, the alignment accuracy of the blood cell analyzer and the kit is improved, the electrical connection stability of the kit and the blood cell analyzer is improved, the possibility of failure, such as undetectable, caused by inaccurate alignment of the kit and the blood cell analyzer is reduced, and the user experience is improved.

Description

Technical Field

[0001] This application relates to the field of in vitro diagnostic technology, and in particular to reagent kits and blood cell analyzers. Background Technology

[0002] Blood cell analyzers are among the most widely used instruments in hospital clinical testing. In order to ensure that the traces of the previous blood sample are completely cleaned before the next blood sample test, traditional blood cell analyzers usually require a large space to set up a cleaning system. The cleaning system is not only complex in structure and has many components, but the cleaning process also requires a large amount of reagents and takes a long time, making traditional blood cell analyzers unsuitable for point-of-care testing scenarios.

[0003] POCT blood cell analyzers have greatly simplified instrument components compared to traditional blood cell analyzers, reducing product complexity and production costs. This allows POCT blood cell analyzers to perform rapid testing immediately at the sampling site, and the operation is simple and quick.

[0004] However, existing POCT hematology analyzers have a front cisternose electrode and a rear cisternose electrode on both sides of the reagent kit when receiving the kit. The hematology analyzer needs to make electrical connections with the front cisternose electrode and the rear cisternose electrode on both sides of the kit. The kit and the hematology analyzer need to be positioned at two connection points, which makes the electrical connection between the kit and the hematology analyzer unstable. Summary of the Invention

[0005] To address the aforementioned problems in the prior art, this application provides a reagent kit and a blood cell analyzer.

[0006] To address the technical problems existing in the prior art, this application provides a reagent kit, including a housing, a detection cell, a microporous sheet, a front cell electrode, and a rear cell electrode; the detection cell is disposed in the housing and includes a front cell and a rear cell, the rear cell being disposed on one side of the front cell; the microporous sheet is located between the front cell and the rear cell, the microporous sheet having micropores, the front cell and the rear cell communicating through the micropores; the front cell electrode and the rear cell electrode, one end of the rear cell electrode being located in the rear cell, one end of the front cell electrode being located in the front cell, and the other end of one of the front cell electrodes extending beyond the microporous sheet to the side where the other electrode is located, while the other end of the other electrode is exposed on the same side of the housing.

[0007] Optionally, one end of the forecell electrode extends beyond the microporous sheet and is exposed on the same side of the housing together with the other end of the rearcell electrode.

[0008] Optionally, the forecell electrode and the rearcell electrode expose equal lengths of the housing.

[0009] Optionally, the rear cistern electrode and the front cistern electrode are arranged along the direction of gravity of the reagent kit.

[0010] Optionally, the kit includes a connector and a pressure chamber, the pressure chamber being in communication with the rear chamber, the connector being connected to the pressure chamber, and one end of the connector, the front chamber electrode, and the rear chamber electrode being exposed on the same side of the housing.

[0011] Optionally, the pressure chamber and the rear pool are arranged along the direction of gravity of the reagent kit.

[0012] Optionally, the bottom of the forecell is provided with a first opening, one end of the forecell electrode extends to the position of the forecell corresponding to the first opening, and the other end of the forecell electrode extends beyond the microporous sheet.

[0013] Optionally, the rear pool includes a first pool body, a second pool body, and a cover. The first pool body is connected to the second pool body, and the cover is installed on one end of the housing near the rear pool electrode. The first pool body, the second pool body, and the cover are arranged to form the rear pool. The cover includes a cover and an abutment. One end of the abutment is connected to the cover, and the other end of the abutment abuts against the microporous sheet.

[0014] To address the technical problems existing in the prior art, this application provides a blood cell analyzer, including a detection holder. The detection holder is used to mount the reagent kit described above; the detection holder includes a power supply assembly for contacting the pre-cell electrode and the post-cell electrode, and the power supply assembly is used to provide a detection current to the reagent kit, enabling the blood cell analyzer to detect the sample to be tested within the reagent kit.

[0015] To address the technical problems existing in the prior art, this application provides a blood cell analyzer, including a detection socket and a power supply assembly. The detection socket is used to receive the reagent kit as described above, and the detection socket is also used to move the reagent kit to the detection position; the power supply assembly is used to interface with the pre-cell electrode and the post-cell electrode at the detection position, and the power supply assembly is used to provide detection current to the reagent kit, so that the blood cell analyzer can detect the sample to be tested in the reagent kit.

[0016] Optionally, the housing includes a first side and a second side, the first side and the second side being disposed along the installation direction or movement direction of the reagent kit, the second side being located in front of the first side, and both the forecell electrode and the rear cell electrode being exposed on the second side of the housing.

[0017] Compared with the prior art, the reagent kit provided in this application has one end of one of the pre-cell electrode and the post-cell electrode extending beyond the microporous sheet to the side where the other electrode is located, while the other end of the other electrode is exposed on the same side of the housing. This allows the reagent kit to be aligned with the pre-cell electrode and the post-cell electrode in one direction when electrically connected to the hematology analyzer. The alignment process between the reagent kit and the hematology analyzer is simple, improving the alignment accuracy between the hematology analyzer and the reagent kit, thereby improving the electrical connection stability between the reagent kit and the hematology analyzer, reducing the possibility of malfunctions such as failure to detect due to inaccurate alignment, and improving the user experience. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of one embodiment of the reagent kit provided in this application;

[0020] Figure 2 yes Figure 1 A cross-sectional view of the reagent kit at section AA;

[0021] Figure 3 This is a schematic diagram of another embodiment of the reagent kit provided in this application;

[0022] Figure 4 This is a schematic diagram of the structure of another embodiment of the reagent kit provided in this application;

[0023] Figure 5 yes Figure 3 Structural diagram of the middle cover assembly;

[0024] Figure 6 This is a schematic diagram of the structure of an embodiment of the blood cell analyzer provided in this application;

[0025] Figure 7 This is a schematic diagram of another embodiment of the blood cell analyzer provided in this application. Detailed Implementation

[0026] The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be particularly noted that the following embodiments are for illustrative purposes only and do not limit the scope of the application. Similarly, the following embodiments are only some, not all, embodiments of the present application, and all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of the present application.

[0027] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0028] In the description of this application, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "setting," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or connections through an intermediate medium. For those skilled in the art, if directional indicators (such as up, down, left, right, front, back, etc.) are involved in the embodiments of this application, these directional indicators are only used to explain the relative positional relationships and movement of the components in a specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indicators will also change accordingly.

[0029] This application first proposes a reagent kit for use in a blood cell analyzer, specifically a POCT blood cell analyzer, for point-of-care testing scenarios. The reagent kit is used to store the sample to be tested, and the blood cell analyzer is used to receive the reagent kit and test the sample. The blood cell analyzer can be used to analyze major examination items in a complete blood count, including red blood cells, white blood cells, and platelets, to obtain detection data of relevant indicators related to these major examination items.

[0030] For example, the test data may include data on leukocyte lineages, such as white blood cell count (WBC), granulocyte number, granulocyte percentage, mid-population cell count, mid-population cell percentage, lymphocyte count, and lymphocyte percentage. The test data can also include data on the erythrocyte lineage, such as red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MCHC), coefficient of variation of red blood cell distribution width (RDW-CV), and standard deviation of red blood cell distribution width (RDW-SD). The test data can also include data on the platelet lineage, such as platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), large platelet count (P-LCR), large platelet ratio (P-LCC), and plateletcrit (PCT).

[0031] Please see Figure 1-2 , Figure 1 This is a schematic diagram of the structure of one embodiment of the reagent kit provided in this application. Figure 2 yes Figure 1 A cross-sectional view of the reagent kit at section AA. (See diagram below.) Figure 1-2 As shown, the kit 10 of this embodiment includes a housing 110, a detection cell 210, a microporous sheet 310, a front cell electrode 212, and a rear cell electrode 214.

[0032] Specifically, the detection cell 210 is disposed in the housing 110, including a front cell 211 and a rear cell 213, with the rear cell 213 disposed on one side of the front cell 211; a microporous plate 310 is located between the front cell 211 and the rear cell 213, and the microporous plate 310 has micropores, through which the front cell 211 and the rear cell 213 are connected; wherein, one end of the front cell electrode 212 is located in the front cell 211, and one end of the rear cell electrode 214 is located in the rear cell 213, and the other end of one of the front cell electrode 212 and the rear cell electrode 214 extends beyond the microporous plate 310 to the side where the other electrode of the front cell electrode 212 and the rear cell electrode 214 is located, and is exposed on the same side of the housing 110 with the other end of the other electrode, so that the other ends of the front cell electrode 212 and the rear cell electrode 214 can be electrically connected to the blood cell analyzer.

[0033] The microwell plate 310 is located between the pre-cell and post-cell chamber 211, specifically on the adjacent sidewalls of the pre-cell and post-cell chamber 213. Microwells are formed on the microwell plate 310, and the area near the microwells is the detection area of ​​the hematology analyzer. The reagent kit 10 is connected to the hematology analyzer, which is used to detect the sample flowing through the microwell plate 310. The power supply component of the hematology analyzer is connected to the pre-cell electrode 212 and post-cell electrode 214 of the detection cell 210, and supplies power to the constant current circuit of the detection cell 210, ensuring that the detection cell 210 operates under a constant current environment.

[0034] One end of the front cell electrode 212 is located within the front cell 211, and one end of the rear cell electrode 214 is located within the rear cell 213, such that the front cell electrode 212 contacts and conducts electricity with the first detection sample within the front cell 211. One end of one of the electrodes, the front cell electrode 212 and the rear cell electrode 214, extends beyond the microporous sheet 310 and reaches the side where the other electrode of the front cell electrode 212 and the rear cell electrode 214 is located. The other ends of the front cell electrode 212 and the rear cell electrode 214 are both exposed on the same side of the housing 110. Specifically, one end of one electrode is located on one side of the microporous sheet 310, and the other electrode is located on the other side of the microporous sheet 310. That is, one end of the front cell electrode 212 and the rear cell electrode 214 are located on opposite sides of the microporous sheet 310, and the other ends of the front cell electrode 212 and the rear cell electrode 214 are located on the other side of the microporous sheet 310.

[0035] For example, in one embodiment, when the position of the front pool 211 is close to the front pool electrode 212 and the rear pool electrode 214 exposed on the side of the housing 110, the electrodes exposed on the side of the housing 110, the front pool 211 and the rear pool 213 are arranged sequentially along the length of the kit 10. At this time, one end of the rear pool electrode 214 is located on one side of the microporous sheet 310, and one end of the front pool electrode 212, the other end of the front pool electrode 212 and the other end of the rear pool electrode 214 are all located on the other side of the microporous sheet 310. That is, the front pool electrode 212 is located on the side of the microporous sheet 310 close to the side of the electrode exposed on the housing 110, and one end of the rear pool electrode 214 is disposed in the rear pool 213 beyond the microporous sheet 310. In another embodiment, when the position of the rear chamber 213 is close to the front chamber electrode 212 and the rear chamber electrode 214 exposed on the side of the housing 110, the electrodes exposed on the side of the housing 110, the rear chamber 213 and the front chamber 211 are arranged along the length of the kit 10. At this time, one end of the front chamber electrode 212 is located on one side of the microporous sheet 310, and one end of the rear chamber electrode 214, the other end of the rear chamber electrode 214 and the other end of the front chamber electrode 212 are all located on the other side of the microporous sheet 310. That is, the rear chamber electrode 214 is located on the side of the microporous sheet 310 close to the side of the electrode exposed on the housing 110, and one end of the front chamber electrode 212 is disposed in the front chamber 211 beyond the microporous sheet 310.

[0036] Because blood cells are poor conductors relative to the diluent, when the first test sample in the pre-cell 211 flows through the micropores to the post-cell 213 under power drive, the resistance between the pre-cell electrode 212 and the post-cell electrode 214 changes as the blood cells of the first test sample flow through the micropores. This generates a voltage change across the pre-cell electrode 212 and the post-cell electrode 214, forming a pulse signal. The blood cell analyzer records the pulse signal and determines the type, volume, and number of blood cells based on the set threshold to obtain test data.

[0037] Furthermore, the other ends of the pre-cell electrode 212 and the rear-cell electrode 214 are exposed on the same side of the housing 110. The housing 110 may include a first side, a second side, a third side, and a fourth side formed by enclosure. The first side is located on the side of the housing 110 near the pre-cell 211 and the rear-cell 213. The second side is opposite to the first side. The third side is adjacent to both the first and second sides. The fourth side is opposite to the third side. In an optional embodiment, the other ends of the pre-cell electrode 212 and the rear-cell electrode 214 may be exposed at any position on the first, second, third, and fourth sides. In an optional embodiment, the pre-cell electrode 212 and the rear-cell electrode 214 may be configured as columnar structures extending along the length of the reagent kit 10. The pre-cell electrode 212 and the rear-cell electrode 214 may also be electrode spring sheets, bent in a preset direction inside the reagent kit 10, so that the user can adjust the exposure position of the pre-cell electrode 212 and the rear-cell electrode 214 according to actual needs. No specific limitation is made here.

[0038] In this embodiment, since one end of one of the front chamber electrode 212 and the rear chamber electrode 214 of the reagent kit 10 extends beyond the microporous plate 310 to the side where the other electrode is located, and the other end of the other electrode is exposed on the same side of the housing 110, the reagent kit 10 can be aligned with the front chamber electrode 212 and the rear chamber electrode 214 in one direction when electrically connected to the hematology analyzer. The alignment process between the reagent kit 10 and the hematology analyzer is simple, improving the alignment accuracy between the hematology analyzer and the reagent kit 10, thereby improving the electrical connection stability between the reagent kit 10 and the hematology analyzer, reducing the possibility of failures such as inability to detect due to inaccurate alignment, and improving the user experience.

[0039] Furthermore, the reagent kit 10 may also include at least one non-detection cell, which can be used to store reagents required for detection such as diluent, hemolysin, and washing solution, and can also be used to provide support for components such as pipette tips for the pipetting assembly of the hematology analyzer. The detection cell 210 and the non-detection cell of the reagent kit 10 are arranged side by side along the length direction of the reagent kit 10 (i.e., the arrangement direction of the front cell 211 and the rear cell 213) on the side of the front cell 211 away from the rear cell 213. That is, the detection cell 210 and at least one non-detection cell of the reagent kit 10 are linearly arranged to reduce the volume of the reagent kit 10 in the width direction. At this time, the front cell electrode 212 and the rear cell electrode 214 exposed on the side of the housing 110 can be the side of the housing 110 facing each other in the length direction, so that the other end of the front cell electrode 212 and the rear cell electrode 214 can be electrically connected to the hematology analyzer. Furthermore, since the various pools of the reagent kit 10 are arranged linearly, the pipetting components of the hematology analyzer can perform pipetting operations between the pools through one-dimensional movement. The hematology analyzer does not need to be set with multiple directional movement tracks, which further reduces the size of the hematology analyzer and is conducive to the miniaturization of the hematology analyzer.

[0040] In one embodiment, one end of the front chamber electrode 212 extends beyond the microporous plate 310, and the other ends of the front chamber electrode 212 and the rear chamber electrode 214 are exposed on the same side of the housing 110.

[0041] Specifically, the rear cell electrode 214 is located on the side of the microporous plate 310 away from the front cell 211, one end of the front cell electrode 212 extends beyond the microporous plate 310 and is located within the front cell 211, and the other ends of the front cell electrode 212 and the rear cell electrode 214 are exposed on the same side of the housing 110. That is, the rear cell electrode 214 is located on the side of the microporous plate 310 near the electrode exposed on the side of the housing 110. In an optional embodiment, the front pool 211 and the rear pool 213 are arranged along the length of the reagent kit 10. The housing 110 includes a first side, a second side, a third side, and a fourth side that are enclosed by the housing. The first side is located on the side of the rear pool 213 away from the front pool 211. The second side is disposed opposite to the first side. The third side is adjacent to the first side and the second side, respectively. The fourth side is disposed opposite to the third side. In this case, the other end of the front pool electrode 212 and the other end of the rear pool electrode 214 can be exposed at any position on the first side, the second side, or the third side. Alternatively, the other end of the front pool electrode 212 and the other end of the rear pool electrode 214 can both be exposed on the first side to reduce the complexity of the internal structure.

[0042] In this embodiment, one end of the pre-cell electrode 212 extends beyond the microporous plate 310, and the other ends of the pre-cell electrode 212 and the rear cell electrode 214 are exposed on the same side of the housing 110. This allows the reagent kit 10 to be aligned with the pre-cell electrode 212 and the rear cell electrode 214 in one direction when electrically connected to the hematology analyzer. The alignment process between the reagent kit 10 and the hematology analyzer is simple, improving the alignment accuracy between the hematology analyzer and the reagent kit 10. This, in turn, improves the electrical connection stability between the reagent kit 10 and the hematology analyzer, reduces malfunctions such as inability to detect due to inaccurate alignment, and improves the user experience.

[0043] In one embodiment, the forecell electrode 212 and the rearcell electrode 214 are exposed to the housing 110 with equal lengths.

[0044] Specifically, the lengths of the front chamber electrode 212 and the rear chamber electrode 214 exposed above the housing 110 are equal, so that the blood cell analyzer can dock with the front chamber electrode 212 and the rear chamber electrode 214 on the same contact surface through the power supply assembly. This reduces the possibility of malfunctions such as poor contact at certain positions when the lengths of the front chamber electrode 212 and the rear chamber electrode 214 exposed above the housing 110 are unequal, and improves the electrical connection stability between the reagent kit 10 and the blood cell analyzer.

[0045] In one embodiment, the rear chamber electrode 214 and the front chamber electrode 212 are arranged along the direction of gravity of the kit 10.

[0046] Specifically, in this embodiment, the rear chamber electrode 214 and the front chamber electrode 212 are arranged along the direction of gravity of the reagent kit 10, that is, the rear chamber electrode 214 is located above the front chamber electrode 212. Correspondingly, the positions where the power supply component docks with the rear chamber electrode 214 and the front chamber electrode 212 are arranged vertically, so that the blood cell analyzer can make an electrical connection with the reagent kit 10 when it receives the reagent kit 10 stably, thereby improving the stability of the electrical connection between the reagent kit 10 and the blood cell analyzer.

[0047] In one embodiment, the bottom of the forecell 211 is provided with a first opening, one end of the forecell electrode 212 extends to the position of the forecell 211 corresponding to the first opening, and the other end of the forecell electrode 212 passes over the microporous plate 310.

[0048] Specifically, the bottom of the front chamber 211 is provided with a first opening, and one end of the front chamber electrode 212 extends to the position of the front chamber 211 corresponding to the first opening, so that one end of the front chamber electrode 212 contacts and conducts electricity with the first detection sample in the front chamber 211. The bottom of the front chamber 211 is sealed by one end of the front chamber electrode 212 to maintain the relative stability of the first detection sample in the front chamber 211. One end of the rear chamber electrode 214 is located in the rear chamber 213, and the other end of the front chamber electrode 212 passes through the microporous plate 310 and is exposed in the housing 110.

[0049] Furthermore, the hematology analyzer is equipped with a first detection component for impedance detection and a second detection component for optical detection. The first detection component performs impedance detection on the sample flowing through the microporous plate 310, and the second detection component performs colorimetric detection on the sample in the pre-cell 211. Since one end of the pre-cell electrode 212 extends to the position in the pre-cell 211 corresponding to the first opening, the contact point between the pre-cell electrode 212 and the first sample is located at the bottom of the pre-cell 211. The detection position of the second detection component when detecting the first sample in the pre-cell 211 is located in the upper middle part of the pre-cell 211. This results in the detection areas of the first and second detection components being staggered, thereby reducing the influence of impedance detection on optical detection and improving the detection accuracy of the hematology analyzer.

[0050] In one embodiment, please refer to Figure 3-5 , Figure 3 This is a schematic diagram of another embodiment of the reagent kit provided in this application. Figure 4 This is a schematic diagram of the structure of another embodiment of the reagent kit provided in this application. Figure 5 yes Figure 3 A structural schematic diagram of the middle cover assembly. (See diagram below.) Figure 3-5 As shown, the rear pool 213 includes a first pool body 2131, a second pool body 2132, and a cover 215. The first pool body 2131 is connected to the second pool body 2132. The cover 215 is installed on the end of the housing 110 near the rear pool electrode 214. The first pool body 2131, the second pool body 2132, and the cover 215 surround each other to form the rear pool 213. The cover 215 includes a cover body 216 and an abutment 217. One end of the abutment 217 is connected to the cover body 216, and the other end of the abutment 217 abuts against the microporous sheet 310.

[0051] Specifically, the first pool 2131 and the second pool 2132 are interconnected and arranged along the direction of gravity. The second pool 2132 is used to accommodate the rear pool electrode 214. When the first detection sample in the front pool 211 flows to the rear pool 213 through the micropores on the microporous sheet 310, the waste liquid of the first detection sample moving from the front pool 211 flows to the first pool 2131 through the second pool 2132, so that the first pool 2131 is used to store the waste liquid of the detection pool 210. The cover 215 is used to cooperate with the first pool 2131 and the second pool 2132. The cover 215 is installed at the end of the housing 110 away from the front pool 211, so that the first pool 2131, the second pool 2132 and the cover 215 surround the rear pool 213. At this time, the second pool wall of the rear pool 213 in the above embodiment is equivalent to the cover 215 in this embodiment. The cover 215 includes a cover body 216 and an abutment 217. The waste liquid flowing from the front pool 211 is located between the first pool body 2131, the second pool body 2132 and the cover body 216. One end of the abutment 217 is connected to the cover body 216, and the other end of the abutment 217 abuts against the microporous plate 310, so that the position of the microporous plate 310 is relatively fixed and the stability of the microporous plate 310 is improved.

[0052] In one embodiment, a first pool body 2131 and a cover 215 are enclosed to form a first cavity, and a second pool body 2132 and a cover 215 are enclosed to form a second cavity. An abutment 217 is disposed in the second cavity. The first cavity is located on the side of the second cavity away from the direction of gravity. The first pool body 2131 includes a first surface 213a connected to the second pool body 2132. The angle between the first surface 213a and the direction of gravity is between 15° and 75°.

[0053] Specifically, the first pool body 2131 and the cover member 215 enclose each other to form a first cavity, which is used to store the waste liquid of the first test sample flowing from the front pool 211. The second pool body 2132 and the cover member 215 enclose each other to form a second cavity. The position of the second cavity corresponds to the position of the microporous plate 310. When the cover member 215 is installed on the housing 110, the abutment member 217 of the cover member 215 is disposed in the second cavity. The cross-sectional area of ​​the first pool body 2131 is larger than the cross-sectional area of ​​the second pool body 2132. At the connection between the first pool body 2131 and the second pool body 2132, the first pool body 2131 includes a first surface 213a and a second surface 213b for connecting the second pool body 2132. The first surface 213a and the second surface 213b are symmetrical about the central axis of the second pool body 2132.

[0054] The angle between the first surface 213a and the direction of gravity is between 15° and 75°. Specifically, this angle can be 15°, 20°, 25°, 30°, 35°, 40°, 45°, 50°, 55°, 60°, 65°, 70°, or 75°. The angle between the second surface 213b and the direction of gravity is equal to the angle between the first surface 213a and the direction of gravity, and will not be described further here. Since the angle between the first surface 213a and the direction of gravity is between 15° and 75°, the first surface 213a and the second surface 213b are inclined surfaces, the bottom of the first pool 2131 has a slope, and the cross-sectional area of ​​the first pool 2131 is larger than that of the second pool 2132, when the first detection sample in the front pool 211 flows from the microporous sheet 310 to the rear pool 213, the waste liquid flowing from the second pool 2132 to the first pool 2131 can reduce the violent vibration caused by the liquid breaking tension. This improves the stability of the first detection component when acquiring signals between the front pool electrode 212 and the rear pool electrode 214, thereby improving the detection accuracy of the blood cell analyzer.

[0055] In one embodiment, the abutment 217 is sleeved on the rear cell electrode 214. A first end face 217a and a second end face 217b are formed in the radial direction of the abutment 217. A flow channel is formed between the first end face 217a and the second end face 217b. The first detection sample of the front cell 211 flows through the flow channel to the rear cell 213. The included angle between the first end face 217a and the second end face 217b is between 20° and 60°.

[0056] Specifically, the abutment 217 can be made of elastic materials such as plastic or rubber. The abutment 217 is sleeved on the rear cell electrode 214 and used to wrap the rear cell electrode 214 to hold the rear cell electrode 214 on a preset axis. The abutment 217 has a first end face 217a and a second end face 217b in the radial direction of wrapping the rear cell electrode 214. A flow channel is formed between the first end face 217a and the second end face 217b, so that part of the rear cell electrode 214 is exposed from the flow channel. The first detection sample of the front cell 211 flows through the flow channel to the rear cell 213. That is, an opening is formed between the first end face 217a and the second end face 217b of the abutment 217, and the rear cell electrode 214 is exposed through the opening.

[0057] Long-term research has revealed that when the angle between the first end face 217a and the second end face 217b is greater than 60°, the opening between them is too large, resulting in dead zones in the flow channel. This prevents the liquid from completely filling the channel, potentially causing air bubbles to form and affecting the accuracy of the test results. Furthermore, an excessively large opening between the first end face 217a and the second end face 217b increases the capacity of the flow channel, slowing down the rate at which the liquid fills the channel and increasing the required volume of the sample. This leads to increased reagent consumption, which is detrimental to cost control of the reagent kit 10. Conversely, when the angle between the first end face 217a and the second end face 217b is less than 20°, the opening is too small. This results in high liquid breaking tension as the sample flows from the forecell 211 into the flow channel, making it prone to air bubble accumulation. This affects the collection of conductive signals by the first detection component, reducing the accuracy of the test results. Therefore, in this embodiment, the included angle between the first end face 217a and the second end face 217b of the reagent kit 10 is within the range of 20°-60°. Specifically, the included angle between the first end face 217a and the second end face 217b can be 20°, 22°, 24°, 26°, 28°, 30°, 32°, 34°, 36°, 38°, 40°, 42°, 44°, 46°, 48°, 50°, 52°, 54°, 56°, 58° or 60°, in order to reduce the influence of air bubbles on the flow channel and improve the detection accuracy of the blood cell analyzer.

[0058] In one embodiment, the cover 216 is provided with a third opening corresponding to the position of the abutment 217. The abutment 217 is provided with an annular body 2171 at one end near the microporous plate 310. One end of the rear cell electrode 214 is exposed on the side wall of the rear cell 213 through the third opening, and the other end of the rear cell electrode 214 abuts against the annular body 2171. When the test sample moves from the front cell 211 to the rear cell 213, the test sample contacts the rear cell electrode 214 through the hole in the middle of the annular body 2171. This allows the rear cell electrode 214 to conduct electricity to the test sample when connected to the power supply component of the blood cell analyzer. The blood cell analyzer counts and detects the first test sample passing through the microporous plate 310. Furthermore, the abutment 217 abuts against the microwell sheet 310 through the annular body 2171, so as to conduct electricity to the test sample and press the microwell sheet 310 tightly at the position of the first opening of the front pool 211, so that the position of the microwell sheet 310 is relatively fixed, reducing the impact of the first test sample flowing from the front pool 211 to the rear pool 213 on the microwell sheet 310, thereby improving the detection stability of the kit 10.

[0059] And / or, the detection cell 210 also includes a sealing element (not shown) for sealing the microporous sheet 310. A second opening is provided on the side wall of the front cell 211 near the rear cell 213. The microporous sheet 310 is provided corresponding to the second opening. The sealing element is located between the microporous sheet 310 and the front cell 211.

[0060] Specifically, a second opening is provided on the side wall of the front pool 211 near the rear pool 213. A sealing element is located between the microporous plate 310 and the side wall of the front pool 211. The sealing element is used to seal the microporous plate 310, ensuring the sealing performance between the microporous plate 310 and the second opening. This allows the first detection sample to flow from the front pool 211 to the rear pool 213 only through the micropores on the microporous plate 310. This improves the stability of the first detection component when acquiring signals between the front pool electrode 212 and the rear pool electrode 214.

[0061] In an optional embodiment, the rear chamber electrode 214 is a columnar electrode, and the central axis of the abutment 217, the central axis of the rear chamber electrode 214, and the central axis of the microporous plate 310 are all on the same straight line. That is, the abutment 217, the rear chamber electrode 214, and the microporous plate 310 are coaxially arranged to press the microporous plate 310 onto the first opening of the front chamber 211, thereby further improving the stability of the microporous plate 310.

[0062] In one embodiment, the kit 10 includes a connection port 313 and a pressure chamber 311. The pressure chamber 311 is in communication with the rear chamber 213. The connection port 313 is connected to the pressure chamber 311. The connection port 313, the other end of the front chamber electrode 212, and the other end of the rear chamber electrode 214 are all exposed on the same side of the housing 110.

[0063] Specifically, the pressure chamber 311 provides driving force for the first test sample in the pre-pool 211, causing the first test sample to flow from the pre-pool 211 to the post-pool 213 under pressure through the microporous sheet 310 for impedance detection of the first test sample in the reagent kit 10. One end of the connector 313 is connected to the pressure source of the hematology analyzer, and the other end of the connector 313 is connected to the pressure chamber 311. The connector 313 is used to provide pressure to the pressure chamber 311 when the pressure source is turned on. In an optional embodiment, the pressure source of the hematology analyzer is a negative pressure source. When the negative pressure source is turned on, the connector 313 is used to adjust the pressure environment of the pressure chamber 311 and the post-pool 213 to a negative pressure environment, causing the first test sample in the pre-pool 211 to flow to the post-pool 213 under the drive of negative pressure.

[0064] In this embodiment, the other end of the connection port 313, the front chamber electrode 212, and the rear chamber electrode 214 are all exposed on the same side of the housing 110. For example, the other end of the connection port 313, the front chamber electrode 212, and the rear chamber electrode 214 are all exposed at any position on the second, third, and fourth sidewalls of the rear chamber 213. This allows the blood cell analyzer to connect to the reagent kit 10 in only one direction when docking with the reagent kit 10. This facilitates the alignment of the reagent kit 10 and the blood cell analyzer, improves the alignment accuracy of the reagent kit 10, and thus improves the connection stability between the reagent kit 10 and the blood cell analyzer.

[0065] Furthermore, the pressure chamber 311 is located on the side of the rear chamber 213 away from the rear chamber electrode 214. The pressure chamber 311 and the rear chamber 213 are arranged sequentially in the direction of gravity. The pressure chamber 311 does not occupy the area of ​​the reagent kit 10 in the length direction, which simplifies the structure and further reduces the volume of the reagent kit 10.

[0066] Furthermore, the kit 10 also includes a baffle 312 located between the pressure chamber 311 and the rear chamber 213. The pressure chamber 311 is connected to the rear chamber 213 through a fourth opening 314 on the baffle 312, which is offset from the central axis of the rear chamber 213.

[0067] Specifically, the pressure chamber 311 and the rear pool 213 are separated by a baffle 312. The baffle 312 is provided with a fourth opening 314. The pressure chamber 311 is connected to the rear pool 213 through the fourth opening 314, so that pressure changes in the pressure chamber 311 can be transmitted to the rear pool 213 through the fourth opening 314. The fourth opening 314 of the baffle 312 is offset from the central axis of the rear pool 213. The central axis of the rear pool 213 is defined as the central axis when facing the first or second pool wall (i.e., the cover 215) of the rear pool 213. In this case, the fourth opening 314 can be located on the left or right sides of the central axis of the rear pool 213, that is, the fourth opening 314 can be located on the side of the central axis of the rear pool 213 that is biased towards the third pool wall (left side) or on the side of the central axis of the rear pool 213 that is biased towards the fourth pool wall (right side).

[0068] For example, the fourth opening 314 is located to the left of the central axis of the rear chamber 213. After the reagent kit 10 completes the test, the rear chamber 213 of the reagent kit 10 stores a large amount of waste liquid generated during the test. If the reagent kit 10 tilts to the right due to external force during transport, the liquid level of the waste liquid in the rear chamber 213 is below the plane where the fourth opening 314 is located, or the waste liquid in the rear chamber 213 is slightly higher than the plane where the fourth opening 314 is located and a small amount overflows into the pressure chamber 311. The tilting of the reagent kit 10 has little impact on the rear chamber 213. When the reagent kit 10 tilts to the left, since the pressure chamber 311 and the rear chamber 213 are separated by the baffle 312, the waste liquid in the rear chamber 213 slowly flows through the fourth opening 314 to the pressure chamber 311. The rising speed of the waste liquid level in the pressure chamber 311 is relatively slow, and the tilting of the reagent kit 10 has little impact on the rear chamber 213. The same applies when the fourth opening 314 is located to the right of the central axis of the rear chamber 213, which will not be described in detail here.

[0069] In this embodiment, the baffle 312 is located between the pressure chamber 311 and the rear pool 213. The pressure chamber 311 is connected to the rear pool 213 through the fourth opening 314 on the baffle 312. The fourth opening 314 is offset from the central axis of the rear pool 213. If the reagent kit 10 is tilted or otherwise damaged after the test is completed, the waste liquid in the rear pool 213 needs to flow slowly to the pressure chamber 311 through the fourth opening 314. The rising speed of the waste liquid level in the pressure chamber 311 is relatively slow, which allows the user to adjust the tilting state of the reagent kit 10 in a timely manner, reducing the possibility of leakage of the reagent kit 10 and improving the reliability of the reagent kit 10.

[0070] In one embodiment, the detection cell 210 is located at one end of the housing 110. The reagent kit 10 is connected to a hematology analyzer, which includes a power supply assembly (not shown) and a negative pressure source (not shown). The power supply assembly is used to connect to the front chamber electrode 212 and the rear chamber electrode 214, and to conduct electricity to the detection sample between the front chamber electrode 212 and the rear chamber electrode 214, enabling the hematology analyzer to count and detect the first detection sample flowing through the microporous sheet 310. The negative pressure source is used to connect to the connection port 313 to provide a negative pressure environment for the pressure chamber 311 and the rear chamber 213, so that the first detection sample in the front chamber 211 can flow through the microporous sheet 310 and into the rear chamber 213 under the drive of negative pressure.

[0071] Since the connection port 313, the other end of the front chamber electrode 212, and the other end of the rear chamber electrode 214 are all exposed on the same side wall of the rear chamber 213, the reagent kit 10 in this embodiment is connected to the blood cell analyzer only through one end face, which makes it easy for the blood cell analyzer to align with the reagent kit 10 on the same side, thereby improving the alignment accuracy of the reagent kit 10.

[0072] In one embodiment, the non-detection pool of the kit 10 may include a diluent pool 412, a hemolysin pool 413, a pre-dilution pool 414, a washing pool 415, a pipette tip placement area 416, and a sample pool 417.

[0073] Specifically, the diluent pool 412 is used to store the diluent, the hemolysin pool 413 is used to store the hemolysin, the pre-dilution pool 414 is used as a place for pre-dilution of the sample with the diluent, the washing pool 415 is used to store the washing solution, the pipette tip placement area 416 is used to insert the pipette tip, and the sample pool 417 is used to store the sample to be tested. The detection pool 210, diluent pool 412, hemolysin pool 413, pre-dilution pool 414, washing pool 415, pipette tip placement area 416, and sample pool 417 are arranged side-by-side along the length of the reagent kit 10. The linear arrangement of the pools in the reagent kit 10 allows the pipetting components of the hematology analyzer to perform pipetting operations between the pools through one-dimensional movement. This eliminates the need for multiple directional movement tracks in the hematology analyzer, further reducing its size and facilitating miniaturization and portability.

[0074] Please see Figure 6 , Figure 6 This is a schematic diagram of an embodiment of the blood cell analyzer provided in this application. Figure 6 As shown, in one embodiment, the blood cell analyzer includes a detection base 20.

[0075] Specifically, the test socket 20 is used to mount the reagent kit 10 as described in any of the above embodiments; the test socket also includes a power supply assembly, which is used to contact the pre-cell electrode 212 and the post-cell electrode 214, and to provide a detection current to the reagent kit 10 so that the hematology analyzer can detect the sample to be tested in the reagent kit 10. In this case, when the reagent kit 10 is mounted on the test socket, it can be placed directly on a mounting position on the test socket, with the power supply assembly fixed near the mounting position to provide a detection current to the reagent kit 10 when it contacts the pre-cell electrode 212 and the post-cell electrode 214. In this case, the reagent kit 10 has an mounting direction that corresponds to the mounting position of the test socket, for example, as shown in the image. Figure 6 As shown, the installation position of the test socket is located on one side of the housing 110 of the test socket. When the user installs the test kit 10 into the test socket, the test kit 10 needs to be moved to the installation position along a preset direction. This direction is the installation direction, so as to realize the electrical connection between the test kit 10 and the blood cell analyzer.

[0076] Please see Figure 7 , Figure 7 This is a schematic diagram of another embodiment of the blood cell analyzer provided in this application. Figure 7As shown, in another embodiment, the hematology analyzer includes a test socket 20 and a power supply assembly. The test socket is used to receive the reagent kit 10 from the previous embodiment and is also used to move the reagent kit 10 to the test position; the power supply assembly is used to interface with the front chamber electrode 212 and the rear chamber electrode 214 at the test position, and the power supply assembly is used to provide a detection current to the reagent kit 10 so that the hematology analyzer can detect the sample to be tested within the reagent kit 10.

[0077] Specifically, the detection holder may be provided with a receiving position and a detection position. When the detection holder is in the receiving position, it is used to receive the reagent kit 10. The detection holder is also used to move the reagent kit 10 from the receiving position to the detection position. The power supply component is positioned close to the detection position so that the power supply component can dock with the front chamber electrode 212 and the rear chamber electrode 214 at the detection position. That is, in this embodiment, the blood cell analyzer needs to move the reagent kit 10 to the detection position before docking with the power supply component. At this time, the detection holder moves the reagent kit 10 in a certain direction. The reagent kit 10 moves along this direction to the detection position and docks with the power supply component to achieve electrical connection between the reagent kit 10 and the blood cell analyzer.

[0078] Optionally, the connection port 313, the front chamber electrode 212, and the rear chamber electrode 214 of the reagent kit 10 are all exposed on the same side of the housing 110. When moving or installing the reagent kit 10, the detection seat 20 only needs to connect one side of the reagent kit 10 to the power supply component or pressure source, thereby realizing the electrical connection between the reagent kit 10 and the hematology analyzer, as well as the connection between the reagent kit 10 and the pressure source. The hematology analyzer controls the power supply status of the power supply component and the on / off status of the pressure source to realize impedance detection and / or optical detection of the reagent kit 10.

[0079] Optionally, the housing 110 includes a first side and a second side, which are arranged along the mounting direction or moving direction of the reagent kit 10. The second side is located in front of the first side in the mounting direction or moving direction, and the front cell electrode 212 and the rear cell electrode 214 are both exposed on the second side of the housing 110.

[0080] Since the second side is located in front of the first side in the installation direction, when the user installs the reagent kit 10 on the detection seat, the power supply component can be connected to the front cell electrode 212 and the rear cell electrode 214 when the reagent kit 10 and the detection seat are in place; or, when the detection seat moves the reagent kit 10, since the second side is located in front of the first side in the installation direction, the detection seat stops moving when the power supply component and the reagent kit 10 are in place, so that the detection seat can connect the power supply component and the electrode while moving in position. The method is simple and easy to implement.

[0081] In this embodiment, both the front chamber electrode 212 and the rear chamber electrode 214 are exposed on the second side of the housing 110. The method of electrically connecting the reagent kit 10 and the power supply assembly is simple and convenient, reducing the possibility of failures such as inability to detect due to misalignment between the reagent kit 10 and the blood cell analyzer, and improving the stability of the electrical connection between the reagent kit 10 and the blood cell analyzer.

[0082] Optionally, the hematology analyzer also includes a pipetting assembly for pipetting the various non-detection and detection cells 210 of the reagent kit 10 to add the first test sample and / or the second test sample into the detection cell 210 for testing.

[0083] Specifically, after the detection port 20 receives and connects to the reagent kit 10, the pipetting assembly moves to the pipette tip placement area 416 of the reagent kit 10 and inserts the pipette tip. The pipetting assembly then moves to the diluent pool 412 of the reagent kit 10 and draws the diluent from the diluent pool 412 using the pipette tip to add the diluent to the pre-dilution pool 414 and the detection pool 210. The sample pool 417 of the reagent kit 10 stores the test sample. The pipetting assembly transfers the test sample stored in the sample pool 417 to the pre-dilution pool 414 to obtain the pre-diluted test sample. The pipetting assembly draws the pre-diluted test sample from the pre-dilution pool 414 using the pipette tip and adds the pre-diluted test sample to the pre-pool 211 of the detection pool 210 to obtain a first test sample diluted to a preset multiple. The blood cell analyzer controls the pressure source to open, and the first test sample in the fore chamber 211 flows through the micropores of the microporous sheet 310 to the rear chamber 213 under pressure. When the first test sample passes through the micropores, it generates a pulse. The blood cell analyzer obtains the test data of the sample by acquiring the pulse signal of the microporous sheet 310, so as to complete the test of the first test sample.

[0084] Furthermore, the pipetting assembly is also used to transfer the first test sample in the pre-cell 211 to the non-test cell of the kit 10 in response to the completion of the first test sample test, so that the pipetting assembly can continue to add the second test sample to the pre-cell 211 and test the second test sample, thereby realizing the reuse of the test cell 210.

[0085] Optionally, the blood cell analyzer includes a first detection component for impedance detection and a second detection component for optical detection.

[0086] After the pipetting assembly prepares the first and second test samples through pipetting operations, the hematology analyzer can perform impedance detection on the first test sample through the first detection assembly and optical detection on the second test sample through the second detection assembly; alternatively, the hematology analyzer can perform optical detection on the first test sample through the second detection assembly and impedance detection on the second test sample through the first detection assembly; or alternatively, the hematology analyzer can also perform impedance detection on the first test sample through the first detection assembly, perform colorimetric detection on the second test sample through the second detection assembly, and then continue to perform impedance detection on the second test sample through the first detection assembly to obtain detection data for multiple hematology parameters.

[0087] The above description is merely an embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A reagent kit, characterized in that, include: case; A detection pool is disposed in the housing, including a front pool and a rear pool, wherein the rear pool is disposed on one side of the front pool; A microporous sheet is located between the front pool and the rear pool, and the microporous sheet has micropores, through which the front pool and the rear pool are connected; The front and rear electrodes are provided, with one end of the rear electrode located in the rear chamber and one end of the front electrode located in the front chamber. The other end of one of the front and rear electrodes extends beyond the microporous sheet to the side where the other electrode is located, and is exposed on the same side of the housing as the other end of the other electrode. One end of the forecell electrode extends beyond the microporous sheet and is exposed on the same side of the housing together with the other end of the rearcell electrode. The rear pool includes a first pool body, a second pool body, and a cover. The first pool body is connected to the second pool body, and the cover is installed on the end of the housing near the rear pool electrode. The first pool body, the second pool body, and the cover are arranged to form the rear pool. The cover includes a cover and an abutment. One end of the abutment is connected to the cover, and the other end of the abutment abuts against the microporous sheet. The first pool body and the cover member enclose each other to form a first cavity, and the second pool body and the cover member enclose each other to form a second cavity. The abutment member is disposed in the second cavity. The first cavity is located on the side of the second cavity away from the direction of gravity. The first pool body includes a first surface connected to the second pool body, and the angle between the first surface and the direction of gravity is within 15°. Between 75°.

2. The reagent kit according to claim 1, characterized in that, The forecell electrode and the rearcell electrode have equal lengths exposed in the housing.

3. The reagent kit according to claim 1, characterized in that, The rear chamber electrode and the front chamber electrode are arranged along the direction of gravity of the reagent kit.

4. The reagent kit according to claim 1, characterized in that, The reagent kit includes a connector and a pressure chamber. The pressure chamber is in communication with the rear chamber, and the connector is connected to the pressure chamber. One end of the connector, the front chamber electrode, and the rear chamber electrode are all exposed on the same side of the housing.

5. The reagent kit according to claim 4, characterized in that, The pressure chamber and the rear chamber are arranged along the direction of gravity of the reagent kit.

6. The reagent kit according to claim 1, characterized in that, The bottom of the forecell is provided with a first opening, one end of the forecell electrode extends to the position of the forecell corresponding to the first opening, and the other end of the forecell electrode passes over the microporous sheet.

7. A blood cell analyzer, characterized in that, include: A test socket for mounting the reagent kit as described in any one of claims 1-6; The detection socket includes a power supply assembly for contacting the pre-cell electrode and the post-cell electrode, and for providing detection current to the reagent kit so that the hematology analyzer can detect the sample to be tested in the reagent kit.

8. A blood cell analyzer, characterized in that, include: A detection port for receiving the reagent kit as described in any one of claims 1-6, the detection port further being used to move the reagent kit to a detection position; A power supply assembly is used to interface with the pre-cell electrode and the post-cell electrode at the detection position. The power supply assembly is used to provide detection current to the reagent kit so that the hematology analyzer can detect the sample to be tested in the reagent kit.

9. The blood cell analyzer according to claim 7 or 8, characterized in that, The housing includes a first side and a second side, which are arranged along the installation direction or movement direction of the reagent kit. The second side is located in front of the first side, and the forecell electrode and the rear cell electrode are both exposed on the second side of the housing.

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