Film having protein adsorption resistance and preparation method thereof

An anti-protein adsorption and thin film technology, applied in electrolytic organic production, electrolytic organic material coating, electrolytic components, etc., to achieve the effects of reducing non-specific protein adsorption, good permeability, and strong manufacturability

Inactive Publication Date: 2015-04-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these materials can weaken the immune response to a certain extent in the body, they will still non-specifically adsorb some proteins, allowing subsequent reactions to continue

Method used

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  • Film having protein adsorption resistance and preparation method thereof
  • Film having protein adsorption resistance and preparation method thereof
  • Film having protein adsorption resistance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The film of this embodiment is prepared by the following method:

[0028] (1) Bromination of the enzyme electrode: first place the enzyme electrode in 0.1mol / L, pH=8 phosphate buffered saline (PBS), dissolve 10ul BIBB in 0.5ml dichloromethane, then add the aforementioned PBS dropwise , stirred at 800 rpm for 1 hour; the brominated enzyme electrode was taken out, washed with deionized water, and dried at room temperature;

[0029] (2) Preparation of polymer monomer solution: take 8 g of sulfobetaine methacrylate (SBMA) monomer, dissolve it in 20 ml of PBS with a pH of 7.4 at 0.1 mol / L, and add the catalyst at a final concentration of 5 mmol / l Divalent copper ion solution, add ligand bipyridine (BPY) at a final concentration of 5 mmol / l, mix thoroughly to form a clear solution;

[0030] (3) Electrically induced atom transfer radical polymerization: the brominated enzyme electrode was used as the working electrode, the silver / silver chloride electrode was used as the refe...

Embodiment 2

[0033] The film of this embodiment is prepared by the following method:

[0034] (1) Bromination of the enzyme electrode: first place the enzyme electrode in 0.1mol / L, pH=8 PBS, dissolve 10ul of dibromoisobutyryl bromide (BIBB) in 0.5ml of dichloromethane, and then add it dropwise In the aforementioned PBS, stir at 800 rpm for 1 hour; take out the brominated enzyme electrode, wash it with deionized water, and dry it at room temperature;

[0035] (2) Preparation of polymer monomer solution: Take 8g of SBMA monomer, dissolve it in 20ml of PBS with a pH of 7.4 at 0.1mol / L, add the catalyst divalent copper ion solution at a final concentration of 5mmol / L, and add a solution of divalent copper ions at a final concentration of 5mmol / L Add the ligand tris(2-pyridylmethyl)amine (TPMA) and mix well to form a clear solution;

[0036] (3) Electrically induced atom transfer radical polymerization: the brominated enzyme electrode was used as the working electrode, the silver / silver chlor...

Embodiment 3

[0039] The film of this embodiment is prepared by the following method:

[0040] (1) Bromination of the enzyme electrode: first place the enzyme electrode in 0.2mol / L, pH=8 PBS, dissolve 5ul BIBB in 1ml dichloromethane, add dropwise to the aforementioned PBS, at 800 rpm Stir for 1 hour; take out the brominated enzyme electrode, wash it with deionized water, and dry it at room temperature;

[0041] (2) Preparation of polymer monomer solution: take 1 g of SBMA monomer, dissolve it in 20 ml of PBS with a pH of 8 at 0.2 mol / L, add the catalyst divalent copper ion solution at a final concentration of 5 mmol / L, and add a solution of divalent copper ions at a final concentration of 10 mmol / L Add the ligand BPY and mix well to form a clear solution;

[0042] (3) Electrically induced atom transfer radical polymerization: the brominated enzyme electrode was used as the working electrode, the silver / silver chloride electrode was used as the reference electrode, the platinum wire electr...

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Abstract

The invention discloses a film having protein adsorption resistance and a preparation method thereof. The film is a polymer of a zwitterionic monomer and covers the surface of an enzyme electrode. The film is prepared by polymerizing the zwitterionic monomer by virtue of electrically induced atom transfer free radical polymerization on the surface of an enzyme electrode. The film, which covers the surface of an enzyme electrode, has good protein adsorption resistance and meanwhile, the high-sensitivity detection through a sensor is ensured; an electrochemical means is fully utilized, the process is simple, the manufacturability is strong, the time is short and the film can be used for large-scale preparation; the thickness of the film can be controlled at 10-40 nm and good permeability is achieved; and at most 99% of non-specific protein adsorption can be reduced.

Description

technical field [0001] The invention belongs to the field of polymer synthesis, and relates to a zwitterionic polymer film and an electropolymerization method thereof. Background technique [0002] In recent years, with the development of science and technology, especially the development of microelectronics, electrochemistry, polymer chemistry and other technologies, the development of biosensors has made great progress. For implantable electrodes, the immune rejection of the sensor by the body is an important reason for electrode failure. Immune rejection mainly includes the following processes: (1) Non-specific protein adsorption: occurs in the earliest stage of foreign body implantation, and is the main cause of short-term failure of implanted electrodes; (2) Inflammatory cell infiltration: occurs in several Within hours, inflammatory cells gradually infiltrate into the implant site and secrete related biological factors; (3) Fibrous envelope formation: usually occurs a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C25D9/02C25B3/00
Inventor 叶学松胡一川杨光
Owner ZHEJIANG UNIV
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