Biological enzyme electrode for biosensor, and its prepn. method

A biosensor and bioenzyme technology, which is applied in the direction of instruments, scientific instruments, and material analysis through electromagnetic means, can solve the problems of poor practicability and no comprehensive improvement effect, and achieve improved anti-interference performance, widened measurement linear range, Overcome the effect of interference current

Active Publication Date: 2005-01-12
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on the improved method for expanding the linear range of measurement.
The above methods are limited to improving the performance of a single aspect of the biosensor, without a comprehensive improvement effect, and poor practicability
The Chinese patent with the application number 03257848.2 is a utility model patent applied earlier by the same applicant and the same main inventor of the present invention. It discloses a biosensor that integrates PEM membrane technology, nanotechnology, embedded enzyme technology and protective layer technology The structure of the bio-enzyme electrode used improves the comprehensive effect and improves the practicability, but there is still room for further improvement of its structure and preparation method

Method used

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  • Biological enzyme electrode for biosensor, and its prepn. method
  • Biological enzyme electrode for biosensor, and its prepn. method
  • Biological enzyme electrode for biosensor, and its prepn. method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The gold foil was printed on the carrier made of polycarbonate according to the shape shown in Figure 4 by spraying method to form the substrate of the working electrode and the counter electrode. The size of the carrier is 40 mm in length, 7 mm in width, and 0.2 mm in thickness. The outer side length of the working electrode and the counter electrode is 35mm, the inner side length is 32mm, and the upper end is 1mm. The lower end of the working electrode is a rectangle with a width of 3.5mm and a height of 3mm or a circle with a diameter of 1-3mm. The rectangular part of the lower end of the counter electrode is 2mm wide and 3mm high. . The upper ends of the working electrode and the counter electrode are aligned with one side of the carrier. Polish the lower part of the working electrode with aluminum powder, rinse it with distilled water to make the surface of the electrode as clean as a mirror, and immerse it in polyallylamine, polyethyleneimine, polydiallyl with a ...

Embodiment 2-4

[0064] Carbon, platinum, or silver are used to form the substrates of the working electrode and the counter electrode respectively, and the others are the same as in Example 1.

Embodiment 5-10

[0066] Nano-silver particles, nano-silica particles, nano-copper particles, mixed nano-gold particles and nano-silica particles, mixed nano-gold particles and nano-silica particles and nano-copper particles, mixed nano-gold particles and Nano-silica particles, nano-copper particles and nano-silver particles are replaced by nano-gold particles, and the others are the same as in Example 1.

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Abstract

On substrate of electrode made from organic insulating material, conducting material is printed through spraying method so as to form working electrode and basal body, and a layer of polycarbonate insulator is coated on surfaces of intermediate part between them. Lower part of basal body of working electrode is coated a conversion zone formed through integrated application including techniques of high polymer built up film or ferrocene covering, embedding enzyme, nano technique, and diffusing astriction so as to fabricate biologic enzyme electrode. The invention raises each performance of biologic sensor and practicability integrally.

Description

technical field [0001] The technical scheme of the invention relates to a biochemical electrode used for analyzing materials by testing electrochemical variables, specifically a bioenzyme electrode for a biosensor and a preparation method thereof. Background technique [0002] Biosensor is a new sensor technology developed in recent decades, which has been widely used in clinical diagnosis, industrial control, food and drug analysis, biopharmaceutical development, environmental analysis, military field, biotechnology and biochip. application. However, the bioenzyme electrodes currently available in the market for biosensors such as glucose and cholesterol are all monomolecular membranes, which have low anti-interference performance due to interference currents, short service life due to easy detachment of the enzyme layer, low test sensitivity and narrow linear range of measurement. shortcoming. In order to improve the anti-interference performance of biosensors, US 5,229,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 陈强吴宝艳韩君黄加栋史海滨韩松岩蒋琳洁
Owner NANKAI UNIV
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