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Preparation of disposable carbon-base glucose chip and electrochemical detection method

An electrochemical, disposable technology, applied in the direction of material electrochemical variables, biological testing, material inspection products, etc., can solve the problems of complicated production technology, achieve good chemical stability and biocompatibility, simple preparation method, good application foreground effect

Inactive Publication Date: 2007-02-14
SOUTHEAST UNIV
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Problems solved by technology

However, due to its complicated production technology, it is of great significance to develop a blood glucose detection chip technology with independent intellectual property rights to reduce the detection cost, and it is of great significance for the screening, early clinical diagnosis, curative effect observation and guiding treatment of hyperlipidemia, hyperglycemia and diabetes

Method used

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  • Preparation of disposable carbon-base glucose chip and electrochemical detection method

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Embodiment Construction

[0030] 1). Choose PVC material as the substrate, carve a track on it, clean it thoroughly with NaOH solution and deionized water, and brush it with silver paste for conductivity after drying.

[0031] 2). Printing graphite ink made of polyvinyl alcohol, ultrafine graphite powder, and cellulose diacetate on the above-mentioned silver paste track to form a printed carbon electrode,

[0032] 3). Cover the carbon electrode with a silicone rubber layer, and only expose the conductive terminal. The central dot is the carbon paste electrode, the left side is the Ag / AgCl reference electrode, and the right side is the auxiliary electrode.

[0033] 4). Blending the PVA aqueous solution with glucose oxidase or a mixture of glucose oxidase and ferrocenic acid, and spotting on the surface of the above-mentioned printed carbon electrode, drying under an ultraviolet lamp to make a printed carbon electrode;

[0034] 5). Preparation of enzyme-modified printed carbon electrode chip: polyvinyl a...

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Abstract

A method for preparing carbon-radical glucose chip of disposable type includes applying PVC material as base material then making track on it and coating silver size on it in order to use said size as conduction material, printing prepared graphite ink on said silver size track to form printed carbon electrode, covering carbon electrode by silia gel rubber layer, making aqueous solution with glucose oxidase and dripping the mixture on surface of printed carbon electrode, using PVA as film substrate material to fix enzyme on surface of carbon electrode by mixing said substrate material with enzyme and electronic transmission media.

Description

technical field [0001] The invention relates to the preparation of a disposable carbon-based glucose detection chip, and combines the advantages of electrochemical analysis with the high selectivity and high sensitivity of an enzyme electrode to realize rapid detection of glucose concentration in blood. Background technique [0002] With the improvement of human living standards, the incidence of various metabolic syndromes is increasing year by year, among which the incidence of hyperlipidemia, hyperglycemia and diabetes is on the rise. For example, the number of diabetic patients in the world was 143 million in 1997, and it reached 300 million in 2005, which doubled the number. The incidence of diabetes in my country is also serious, and the number of patients has exceeded 30 million, accounting for 2.5% of the total population. At the same time, there are a considerable number of patients with impaired glucose tolerance (pre-diabetes), and 5%-15% of these patients will c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N27/30
Inventor 刘松琴
Owner SOUTHEAST UNIV
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