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Current enzyme electrode for detecting catalase-positive bacteria and preparation method thereof

A current-type, positive bacteria technology, applied in the field of biosensing, can solve the problems of high detection cost, low stability, complicated operation, etc., and achieve the effect of good repeatability, short detection time and high sensitivity

Inactive Publication Date: 2010-09-08
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to extract the NADH in the bacterial cells first, the operation is complicated, the stability is not high, the detection limit is high, and the detection cost is high, so it is not suitable for large-scale rapid detection on site

Method used

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  • Current enzyme electrode for detecting catalase-positive bacteria and preparation method thereof
  • Current enzyme electrode for detecting catalase-positive bacteria and preparation method thereof
  • Current enzyme electrode for detecting catalase-positive bacteria and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Such as figure 1 and figure 2 As shown, the current-type enzyme electrode electrode prepared in this example for testing catalase-positive bacteria in food includes: electrode substrate 1, connection terminal 2, electrode connection wire 3, working electrode 4, counter electrode 5, insulating Layer 6, reaction substrate film 7, wherein: connecting terminal 2, electrode wire 3, working electrode 4 and counter electrode 5 are arranged between electrode substrate 1 and insulating layer 6 respectively, and working electrode 4 is positioned at the middle of counter electrode 5 and There is a gap, one end of the two electrode wires 3 is respectively connected to the working electrode 4 or the counter electrode 5, the other end of the two electrode wires 3 is respectively connected to the two terminals 2, and the reaction substrate film 7 is located on the working electrode. above electrode 4 and counter electrode 5.

[0039] The electrode substrate 1 is 37mm long, 9mm wide...

Embodiment 2

[0056] In the preparation process of the screen-printed electrode, except that the electrode substrate is made of polystyrene, the others are the same as in Example 1.

Embodiment 3

[0058] In the preparation process of the screen-printed electrode, the screen-printed electrode terminal, electrode connection, working electrode and counter electrode were all printed with conductive carbon paste, and the others were the same as in Example 1.

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Abstract

The invention relates to a current enzyme electrode for detecting catalase-positive bacteria and a preparation method thereof in the technical field of biological sensing; the electrode comprises an electrode substrate, wiring terminals, electrode connection cables, a working electrode, a counter electrode, an insulation layer and reaction substrate films, and is characterized in that the wiring terminals, the electrode connection cables, the working electrode and the counter electrode are respectively arranged between the electrode substrate and the insulation layer; the working electrode is arranged in the middle of the electrode with a gap left between; one end of each electrode connection cable is respectively connected with the working electrode and the counter electrode, and the other end thereof is respectively connected with the two wiring terminals; and the reaction substrate films are arranged above the working electrode and the counter electrode. The electrode which is prepared by the method has the advantages of high sensitivity and no need of bacterial culturing, can realize continuous detection of a plurality of samples by replacing different disposable electrode strips, is simple to operate, is easy for industrial popularization, and has important practical value in food bacterial pollution monitoring.

Description

technical field [0001] The invention relates to a device in the technical field of biosensing and a preparation method thereof, in particular to a current-type enzyme electrode for detecting catase-positive bacteria and a preparation method thereof. Background technique [0002] Catalase-positive bacteria refer to bacteria that can produce catalase, and they are widely found in various foods. Many catalase-positive bacteria, including Escherichia coli, Listeria, Salmonella typhimurium, Vibrio parahaemolyticus, and Staphylococcus aureus, have been etiologically recognized as zoonotic and foodborne pathogens. It is generally recognized worldwide and directly threatens human health. Therefore, establishing a rapid and effective method for detecting Staphylococcus aureus in food has important practical value for ensuring food safety and public health. [0003] At present, the methods for detecting catalase-positive bacteria are mainly traditional bacterial identification metho...

Claims

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Application Information

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IPC IPC(8): G01N27/327C12Q1/04
Inventor 刘国艳柴春彦姚冰刘晓芳
Owner SHANGHAI JIAO TONG UNIV
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