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Enzyme electrode and preparation method thereof

An enzyme electrode and electrode technology, applied in the field of biosensors, can solve problems such as changing enzyme activity, and achieve the effects of good stability, high sensitivity and wide linear range

Inactive Publication Date: 2011-04-20
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of doped semiconductor nanowires to modify enzyme activity has not been reported

Method used

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  • Enzyme electrode and preparation method thereof
  • Enzyme electrode and preparation method thereof
  • Enzyme electrode and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of Sb-doped SnO 2 Nanowires: Put the gold-plated silicon chip into a magnetic boat filled with tin powder and antimony powder, wherein the mass ratio of tin powder and antimony powder is 100:5, at 850°C and 80sccm N 2 Sb-doped SnO can be obtained on the surface of a silicon wafer by heating under airflow conditions for 2 hours 2 Nanowires;

[0030] Doped SnO containing 1 mg / mL of the above Sb 2 The ethanol solution of nanowires and the phosphate buffer solution containing 2 mg / mL HRP were uniformly mixed in equal volumes, and 6 μL was drip-coated on the surface of the glassy carbon electrode treated by the above method, and dried in the refrigerator at 4 ° C to prepare the nanowire / HRP composite Membrane enzyme electrode.

[0031] A three-electrode system was used to test the above-mentioned enzyme electrode, in which the platinum electrode was used as the counter electrode, the Ag / AgCl (saturated KCl) electrode was used as the reference electrode, the ab...

Embodiment 2

[0033] Doped SnO with 2mg of Sb 2The nanowires were dispersed in 1 mL of 1wt% Nafion ethanol solution, mixed evenly with a phosphate buffer solution containing 6 mg / mL myoglobin (Mb) at a volume ratio of 1:0.9, and 7 μL was drip-coated on the gold treated by the above method. The surface of the electrode was dried in a refrigerator at 4° C. to prepare a nanowire / Mb composite film enzyme electrode.

[0034] According to the method in Example 1 with different concentrations of H 2 o 2 The enzyme electrode of Example 2 was tested as a substrate, and the sensitivity had a good linear relationship in the range of 1-600 μM, and the stability was good, and 91% of the activity was still maintained after four weeks of testing.

Embodiment 3

[0036] Sb doped SnO 2 The nanowires were dispersed into an acetic acid solution containing 0.3wt% chitosan (the concentration of acetic acid in the solution was 0.05M), wherein the Sb doped SnO in the solution 2 The concentration of the nanowires is 1 mg / mL, and the solution is uniformly mixed with the phosphate buffer solution containing 2 mg / mL cytochrome C at a volume ratio of 1:1.25, and 8 μL is drip-coated on the surface of the cleaned pyrolytic graphite electrode, and the refrigerator is 4 °C and then dried to prepare the nanowire / cytochrome C composite film enzyme electrode.

[0037] According to the method in Example 1 with different concentrations of H 2 o 2 The enzyme electrode of embodiment 3 is tested as substrate, has very wide linear range (10~400 μ M), very high sensitivity (90mA M -1 cm -2 ) and good stability, the test still maintained 85% of the activity after one month.

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Abstract

The invention discloses an enzyme electrode which belongs to the technical field of biosensors. The enzyme electrode consists of a base electrode and a responding layer fixed on the surface of the base electrode, wherein the responding layer consists of doped semiconductor nanometer wires on which enzyme is fixed. The invention also discloses a preparation method of the enzyme electrode. The preparation method comprises the step of adopting the doped semiconductor nanometer wires to fix enzyme molecules so as to achieve the direct electrochemical performance of the enzyme molecules. The enzyme electrode has better catalytic activity and wider linear range.

Description

technical field [0001] The invention belongs to the field of biosensors, and in particular relates to an enzyme electrode and a preparation method thereof. Background technique [0002] As an analytical device, biosensors have important practical value in the fields of environmental monitoring, clinical testing, food and drug analysis, and biochemical analysis. Among them, enzymes as biocatalysts have excellent characteristics of high efficiency and specificity, making enzyme-based electrochemical biosensors become the mainstream of biosensor research. [0003] The development of electrochemical biosensors has mainly gone through three stages: the first stage is the first generation of biosensors that use oxygen as the electron transport medium, and measure the concentration of the analyte by detecting the consumption of oxygen or the increase of hydrogen peroxide in the system. concentration. Due to its influence by oxygen partial pressure and H 2 o 2 Due to the shortco...

Claims

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Application Information

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IPC IPC(8): G01N27/327B82B3/00
Inventor 李景虹李利淼黄瑾
Owner TSINGHUA UNIV
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