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ZnO nanowire biosensor and preparation method thereof

A biosensor and nanowire technology, applied in instruments, scientific instruments, measuring devices, etc., can solve problems affecting direct electron transfer of enzymes, uneven enzyme membranes, and reduced sensitivity, and achieve easy operation, improved detection sensitivity and accuracy, good orientation effect

Inactive Publication Date: 2010-07-14
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after mixing ZnO nanoparticles with enzymes, the ZnO nanoparticles are completely wrapped by the non-conductive enzymes. In addition, due to the agglomeration of ZnO nanoparticles and other reasons, the enzyme film formed on the surface of the electrochemical electrode is not uniform, which affects the Direct electron transfer between the active center of the enzyme and the electrode, as a biosensor, with reduced sensitivity

Method used

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  • ZnO nanowire biosensor and preparation method thereof
  • ZnO nanowire biosensor and preparation method thereof
  • ZnO nanowire biosensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Make a conductive layer on the substrate

[0021] An ITO layer is grown on a glass substrate to obtain an ITO glass substrate.

[0022] 2. Growth of ZnO nanowires on the conductive layer

[0023] Use hydrothermal method to grow ZnO nanowires according to the following process conditions: use 0.01M zinc acetate and 0.01M hexamethylenetetramine to prepare a reaction solution, the growth temperature is 90°C, the growth time is 6h, and after the growth is completed, take it out at 60°C The grown ZnO nanowires have a nanoscale and ideal aspect ratio, and the morphology tends to be consistent, densely and uniformly clustered on the conductive layer, see figure 1 shown.

[0024] 3. Generating an enzyme coating layer on the surface of ZnO nanowires

[0025] Generating the enzyme coating layer by electrostatic adsorption: use K 2 HPO 4 and KH 2 PO 4 The standard solution was prepared as a 0.01M phosphate buffer solution with a Ph value of 6.86. Prepare glucose oxidase...

Embodiment 2

[0029] 1. Make a conductive layer on the substrate

[0030] A ZnO seed layer is grown on a polyimide substrate by magnetron sputtering to obtain a polyimide substrate with a ZnO seed layer.

[0031] 2. Growth of ZnO nanowires on the conductive layer

[0032] ZnO nanowires were grown by electrochemical deposition: 0.005M zinc nitrate and 0.005M hexamethylenetetramine were used to prepare a growth solution and stirred evenly. The conductive layer on the substrate is used as the working electrode in the three-electrode system, the platinum sheet is used as the auxiliary electrode, and Ag / AgCl is used as the reference electrode. The growth temperature is 90° C., the growth time is 1 h, and the deposition potential is -1 V to complete the growth of ZnO nanowires.

[0033] 3. Generating an enzyme coating layer on the surface of ZnO nanowires

[0034] Generating the enzyme coating layer by electrostatic adsorption: use K 2 HPO 4 and KH 2 PO 4 The standard solution was prepared...

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Abstract

The invention relates to a ZnO nanowire biosensor and a preparation method thereof, belonging to the technical field of nano semiconductor materials. In the prior art, the mixed solution of ZnO nano particles and enzyme are spun on an electrochemical electrode so that an enzyme electrode is made; the ZnO nano particles are fully wrapped by the non-conducting enzyme, and besides, an enzyme film formed on the surface of the electrochemical electrode is uneven, thereby affecting the direct electronic transfer between the active center of the enzyme and the electrode; and therefore, the sensitivity of the electrochemical electrode as a biosensor is reduced. The ZnO nanowire biosensor comprises a substrate, ZnO and the enzyme, wherein a conducting layer is arranged on the substrate, a ZnO nanowire is grown on the conducting layer, and an enzyme-wrapped layer is attached to the surface of the nanowire. The method of the invention comprises the following steps of: firstly making the conducting layer on the substrate, then growing the ZnO nanowire on the conducting layer, and thirdly generating the enzyme-wrapped layer on the surface of the nanowire. The biosensor as a working electrode is used for electrochemical working stations as chemical test devices to realize accurate tests.

Description

technical field [0001] The invention relates to a ZnO nanowire biosensor and a preparation method thereof, belonging to the technical field of nanometer semiconductor materials. Background technique [0002] ZnO is a new wide bandgap, direct bandgap II-VI semiconductor oxide material. It has a wide bandgap energy, the energy band width at room temperature is 3.37eV, it has a large exciton binding energy, reaching 60meV, non-toxic, has high thermal stability, and also has a high isoelectric point, isoelectric point is 9.5. In addition, ZnO also has good biocompatibility and electron transfer properties, which make ZnO not only maintain the biological activity of the immobilized enzyme well, but also improve the direct electron transfer between the active center of the enzyme and the electrode, thereby It can be used to prepare biosensors, such as ZnO nano-biosensors, and it is a kind of bioelectrode used in electrochemical workstations. [0003] The existing ZnO nano-biose...

Claims

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Application Information

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IPC IPC(8): G01N27/327C01G9/02
Inventor 李金华李静方铉王晓华魏志鹏赵东旭
Owner CHANGCHUN UNIV OF SCI & TECH
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