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Oxygen-enriched nano bio-enzyme electrode as well as sensor device, preparation method and application of oxygen-enriched nano bio-enzyme electrode

A sensor device and biological enzyme technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of difficult electrode structure, limited linear range of glucose detection, interference of glucose response signals, etc., and meets the requirements of preparation technology with simple and easy conditions. The effect of mass production, good biocompatibility

Active Publication Date: 2018-11-23
SCITECH CENTRY SUZHOU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Insufficient oxygen concentration limits the linear range of glucose detection;
[0004] 2) The oxygen content in the solution to be tested fluctuates, which limits the detection accuracy of the sensor;
[0005] 3) The detection of hydrogen peroxide usually needs to be carried out at a high potential of >0.6V. However, at this potential, most endogenous interfering substances (ascorbic acid, uric acid, etc.) and reducing drugs (acetaminophen, acetylsalicylic acid, etc.) acid, etc.) can also be oxidized on the electrode, thereby interfering with the glucose response signal and affecting the accuracy of glucose detection
These electronic mediators have certain toxicity to the human body, and are easy to fall off from the electrodes, with poor stability
[0008] 2) The electronic medium will compete with oxygen for electrons, resulting in oxygen interference, which will affect the detection accuracy of the glucose sensor, especially for low-concentration glucose solutions.
[0009] In the prior art, a three-phase oxygen-enriched electrode is used to solve the above problems. One end is in contact with the liquid to be measured, and the other end is in contact with an oxygen gas system. However, although this two-electrode or three-electrode system can be used for continuous measurement, the electrode structure is difficult. It is not easy to operate and carry, and cannot meet people's daily needs and mass production

Method used

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  • Oxygen-enriched nano bio-enzyme electrode as well as sensor device, preparation method and application of oxygen-enriched nano bio-enzyme electrode
  • Oxygen-enriched nano bio-enzyme electrode as well as sensor device, preparation method and application of oxygen-enriched nano bio-enzyme electrode
  • Oxygen-enriched nano bio-enzyme electrode as well as sensor device, preparation method and application of oxygen-enriched nano bio-enzyme electrode

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Experimental program
Comparison scheme
Effect test

Embodiment 2

[0106] Hollow mesoporous silica spheres were prepared by the following method:

[0107] (1) 25ml aqueous solution including 100nm polystyrene microspheres 0.2wt%, cetyltrimethylammonium bromide 0.2wt%, sodium hydroxide 0.056wt% and 1,2-bis(triethoxysilyl) Ethane 0.768wt%, stirred and reacted at 80°C for 2 hours, then centrifuged and washed 3 times with water and ethanol to obtain a sample.

[0108] (2) Drop the polystyrene@mesoporous silica balls washed in (1) on the cleaned glass slide, put them into a tube furnace, and calcine for 6 hours with oxygen at 550°C.

[0109] (3) Scrape the hollow mesoporous silica sphere prepared in (2) from the glass slide with a clean blade, add a certain amount of ethanol or deionized water, and make a certain amount of hollow mesoporous silica sphere ethanol or aqueous solution.

[0110] The above slide cleaning method is to use ethanol: acetone: water as a 1:1:1 organic solvent as a cleaning agent, ultrasonic for 15 minutes, and an oven in ...

Embodiment 3

[0119] Hollow mesoporous silica spheres were prepared by the following method:

[0120] (1) 25ml aqueous solution including 100nm polystyrene microspheres 0.2wt%, cetyltrimethylammonium bromide 0.2wt%, sodium hydroxide 0.056wt% and 1,2-bis(triethoxysilyl) Ethane 0.576wt%, stirred and reacted at 80°C for 2 hours, then centrifuged with water and ethanol for 3 times to obtain a sample.

[0121] (2) Drop the polystyrene@mesoporous silica balls washed in (1) on the cleaned glass slide, put them into a tube furnace, and calcine for 6 hours with oxygen at 550°C.

[0122] (3) Scrape the hollow mesoporous silica sphere prepared in (2) from the glass slide with a clean blade, add a certain amount of ethanol or deionized water, and make a certain amount of hollow mesoporous silica sphere ethanol or aqueous solution.

[0123] The above slide cleaning method is to use ethanol: acetone: water as a 1:1:1 organic solvent as a cleaning agent, ultrasonic for 15 minutes, and an oven in an oven...

Embodiment 4

[0132] Prepare hollow alumina spheres by the following method:

[0133] (1) Dissolve 0.6303g of ammonium formate in 50ml of deionized water, and adjust the pH to 4.4 with formic acid. 3 ml (2.5 wt %) of an aqueous solution of 100 nm polystyrene microspheres and 0.351 g of aluminum sulfate sonicated for 15 minutes were added. After reacting at 70°C for two hours, centrifuge and wash with water and ethanol.

[0134] (2) Drop the polystyrene microspheres@alumina balls washed in (1) on the cleaned glass slide, put them into a tube furnace, and calcinate at 550°C for 2 hours with oxygen.

[0135] (3) Scrape the hollow alumina ball prepared in (2) from the glass slide with a clean blade, add a certain amount of ethanol or deionized water, and make a certain amount of ethanol or aqueous solution of the hollow alumina ball.

[0136] The above slide cleaning method is to use ethanol: acetone: water as a 1:1:1 organic solvent as a cleaning agent, ultrasonic for 15 minutes, and an oven...

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Abstract

The invention provides an oxygen-enriched nano bio-enzyme electrode as well as a sensor device and a preparation method and application of oxygen-enriched nano bio-enzyme electrode. According to the oxygen-enriched nano bio-enzyme electrode, an electrode substrate is modified with a hollow structure nano material with the oxygen enrichment function, catalyst particles of hydrogen peroxide and an oxidase corresponding to a substance to be detected. The oxygen-enriched nano bio-enzyme electrode and the device have the advantages that the detection linear range is wide, the sensitivity is high, the anti-interference performance is good, and in addition, batch production can be carried out; and the advantages are mainly derived from the hollow structure material with the oxygen storage function.

Description

technical field [0001] The invention relates to the technical field of electrode material preparation and electrochemical detection, in particular to an oxygen-enriched nano-biological enzyme electrode material, a sensor device, a preparation method and application thereof. Background technique [0002] Glucose is a worldwide public health problem and has become a dangerous disease second only to cardiovascular disease, namely cancer. Today, there are as many as 300 million people with diabetes in the world, among which there are as many as 100 million people with severe diabetes who need real-time control of blood sugar concentration. Therefore, it is of great significance to realize accurate and rapid detection of blood sugar of diabetic patients in daily life. Since 1962, when Leland Clark first proposed a theoretical model for measuring glucose with an enzyme electrode, the research on glucose sensors has never stopped. Nowadays, amperometric glucose sensors are mainly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 封心建唐鹤鸣
Owner SCITECH CENTRY SUZHOU BIOTECH CO LTD
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