Process for the detecting of hydrogen peroxide

A hydrogen peroxide and detection method technology, applied in the direction of material electrochemical variables, preparation of test samples, etc., can solve problems such as difficult long-term storage, decreased stability, decreased activity, etc., and achieve effective and wide-ranging electrochemical effects of products Detection limit, rapid response effect

Inactive Publication Date: 2009-11-18
NANJING NORMAL UNIVERSITY
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Problems solved by technology

Hydrogen peroxide is generally detected by horseradish peroxidase (HRP) catalyzing the reaction of hydrogen peroxide on the electrode to generate a current response. An electron mediator is used between HRP and the electrode to transfer electrons, and HRP is not only expensive, not easy to store for a long time, but also easy to inactivate, resulting in reduced activity and stability

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  • Process for the detecting of hydrogen peroxide
  • Process for the detecting of hydrogen peroxide

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Embodiment 1

[0027] Embodiment 1, the detection method of hydrogen peroxide,

[0028] (1), prepare nano-scale ferrous sulfide: (NH 4 ) 2 SO 4 ·FeSO 4 ·6H 2 O (0.39121g) adds 25mL nitrogen deoxygenation secondary water, after dissolving, add dodecylamine hydrochloride (0.0776g) and dissolve completely to obtain solution A; Na 2 S.9H 2 O (0.1201g), add 10mL nitrogen deoxygenation secondary water to dissolve, then dissolve dodecylamine hydrochloride in 15mL nitrogen deoxygenation secondary water to obtain a 0.014M solution; then mix Na 2 S.9H 2 O and dodecylamine hydrochloride solution, to obtain a milky suspension B. Under ultrasonication, add solutions A and B successively into a three-necked flask protected by a nitrogen atmosphere, the reaction occurs immediately, the solution turns black, continue ultrasonication for 10 minutes and then centrifuge, successively use deoxygenated secondary water, nitrogen-passed absolute ethanol, and wash three times each Precipitation, during the ...

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Abstract

The detection method of hydrogen peroxide, the steps are: (1), preparation of nano-scale ferrous sulfide: first dissolving ferrous ammonium sulfate in secondary water for nitrogen deoxygenation, then adding the mixed solution of sulfide and surfactant, in Mix under ultrasonic conditions, centrifuge and wash the precipitate after the end of the reaction, and vacuum-dry to obtain a nanoscale ferrous sulfide sample; wherein the mol ratio of sulfide and surfactant agent is 1: 1, and the amount of surfactant is 0.014M; (2 ), detection preparation: drip-coat the suspension of ferrous sulfide nanoparticles and the polymer film successively on the glassy carbon electrode, dry and age; (3), detection of hydrogen peroxide: continuously add peroxide in the electrolytic cell Hydrogen is electrochemically detected, and its ampere response is recorded to obtain the detection result. The present invention uses nano-level ferrous sulfide to replace HRP to detect hydrogen peroxide. Since the nano-material has a large specific surface area, the detection sensitivity is improved, and the purpose of simplicity, speed, sensitivity and stability is achieved.

Description

technical field [0001] The invention relates to a novel, simple, fast, sensitive and stable hydrogen peroxide detection method. Background technique [0002] Hydrogen peroxide is a very important active intermediate in clinical medicine and environmental chemistry. The detection of its concentration has important research significance in the fields of chemical analysis, biological analysis, medicine, clinical diagnosis, and environmental analysis. At present, the methods for the determination of hydrogen peroxide mainly include polarography, flow injection conductometric method, electroanalysis, fluorescence analysis, spectrophotometry and chemiluminescence. Among them, the electroanalysis method is widely used because of its simplicity, rapidity, sensitivity and low price. Hydrogen peroxide is generally detected by horseradish peroxidase (HRP) catalyzing the reaction of hydrogen peroxide on the electrode to generate a current response. An electron mediator is used between...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/42G01N1/28
Inventor 戴志晖包建春倪嘉
Owner NANJING NORMAL UNIVERSITY
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