Method for building three-dimensional enzyme electrode surface by using coaxial nanofibers

Abstract

The invention provides a method for building a three-dimensional enzyme electrode surface by using coaxial nanofibers. The method comprises the following steps: preparing a coaxial electrospinning kernel material by dissolving a high molecular weight polymer and a high-orientation nano material into water; preparing a coaxial electrospinning shell material by dissolving enzymes or proteins with catalytic property and a high molecular weight polymer together into water; spinning nanofibers on an electrode surface by using a coaxial electrospinning method; and cross-linking by using a cross-linking agent to obtain a three-dimensional enzyme electrode. The conditions of the coaxial electrospinning method are that spinning voltage is 8-30kV, liquid flow rate is 0.5-2mL / h, and spinning interval is 10-100cm. According to the method, the coaxial electrospinning technology is used to prepare a coaxial nanofiber film electrode, the preparation of the enzyme electrode is finished by relatively simple steps, the surface enzyme immobilization stability of the enzyme electrode is improved, the response speed of the enzyme electrode is improved, and the output power of a fuel battery is increased.

Description

technical field [0001] The invention relates to a preparation method of an enzyme electrode. Background technique [0002] Enzyme electrodes used in traditional biosensors and biofuel cells are mostly prepared by drop coating, electrochemical deposition, or a combination of the two. In the process of preparing modified electrode nanocomposite membranes by drop coating, nanomaterials are easy to aggregate, which affects the distribution of enzymes on the electrode surface and the orientation of enzymes on the electrode surface, and further affects the overall performance of enzyme electrodes. On the other hand, the application of electrodeposition makes the distribution of film-forming materials on the electrode surface uneven, and the stability and repeatability of the electrode surface are poor. [0003] With the continuous development of nanotechnology, electrospinning technology has attracted more and more researchers' attention. Electrospun fibrous membranes have the a...

AI Technical Summary

Problems solved by technology

However, with the increase in the application of enzyme-modified electrodes, its limitations have also been exposed.

For example, some enzymes will lose their activity after being dissolved in a certain matrix for electrospinning, so they cannot be used in the preparation of direct electrospun enzyme-containing fiber membrane modified electrodes

When the active enzyme is immobilized on the surface of the electrospun fiber membrane, due to the hydrophobicity of part of the fiber surface, the enzyme immobilized on the surface of the fiber membrane has poor stability and is easy to fall off.

If a cross-linking agent is used to further immobilize the enzyme, it is easy to lose the enzyme activity due to macromolecular entrapment

Therefore, in the application process, the electron transfer between the enzyme and the electrode is difficult, and the enzyme is easy to fall off from the fiber surface, resulting in limited improvement in the overall performance of the enzyme electrode.

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