Method for building three-dimensional enzyme electrode surface by using coaxial nanofibers

A nanofiber, electrode surface technology, applied in nanotechnology, nanotechnology, battery electrodes, etc., can solve the problem of inability to use direct electrospun enzyme-containing fiber membranes to modify electrode preparation, difficulty in electron transfer between enzymes and electrodes, and comprehensive performance of enzyme electrodes To improve the stability of the enzyme solidification, increase the electron transfer rate, and solve the stability problem.

A nanofiber, electrode surface technology, applied in nanotechnology, nanotechnology, battery electrodes, etc., can solve the problem of inability to use direct electrospun enzyme-containing fiber membranes to modify electrode preparation, difficulty in electron transfer between enzymes and electrodes, and comprehensive performance of enzyme electrodes To improve the stability of the enzyme solidification, increase the electron transfer rate, and solve the stability problem.

CN102645474AInactive Publication Date: 2012-08-22HARBIN ENG UNIV
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  • Method for building three-dimensional enzyme electrode surface by using coaxial nanofibers
  • Method for building three-dimensional enzyme electrode surface by using coaxial nanofibers

Examples

Experimental program
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Embodiment 1

[0018] In the preparation process, hemoglobin is used as an enzyme with catalytic activity, polyethylene glycol is used as a polymer matrix, and carbon nanotubes are used as a doping phase to improve the conductivity of the inner core. Its preparation process is as follows: 0.005 g of hemoglobin and 0.2 g of polyethylene glycol were dissolved in 2 ml of water as the electrospun shell. 0.2 polyethylene glycol and 0.01 g single-walled carbon nanotubes were dissolved in trifluoroethanol as the electrospun core. The nanofibers were spun on the electrode surface by coaxial electrospinning technique. Spinning voltage: 12kV; liquid flow rate: 1mL / h; spinning distance: 20cm. The obtained electrode is cross-linked by a cross-linking agent for several hours and the electrode required for the experiment is obtained.

Embodiment 2

[0020] Using serum albumin as the enzyme with catalytic activity, polyethylene glycol as the polymer matrix, single-walled carbon nanotubes as the doping phase, and other conditions as in Example 1, a coaxial fiber membrane modified electrode can be prepared.

Embodiment 3

[0022] Using glucose oxidase as the enzyme with catalytic activity, polyethylene glycol as the polymer matrix, single-walled carbon nanotubes as the doping phase, and other experimental conditions as in Example 1, a coaxial fiber membrane modified electrode can be prepared.

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Abstract

The invention provides a method for building a three-dimensional enzyme electrode surface by using coaxial nanofibers. The method comprises the following steps: preparing a coaxial electrospinning kernel material by dissolving a high molecular weight polymer and a high-orientation nano material into water; preparing a coaxial electrospinning shell material by dissolving enzymes or proteins with catalytic property and a high molecular weight polymer together into water; spinning nanofibers on an electrode surface by using a coaxial electrospinning method; and cross-linking by using a cross-linking agent to obtain a three-dimensional enzyme electrode. The conditions of the coaxial electrospinning method are that spinning voltage is 8-30kV, liquid flow rate is 0.5-2mL / h, and spinning interval is 10-100cm. According to the method, the coaxial electrospinning technology is used to prepare a coaxial nanofiber film electrode, the preparation of the enzyme electrode is finished by relatively simple steps, the surface enzyme immobilization stability of the enzyme electrode is improved, the response speed of the enzyme electrode is improved, and the output power of a fuel battery is increased.

Description

technical field [0001] The invention relates to a preparation method of an enzyme electrode. Background technique [0002] Enzyme electrodes used in traditional biosensors and biofuel cells are mostly prepared by drop coating, electrochemical deposition, or a combination of the two. In the process of preparing modified electrode nanocomposite membranes by drop coating, nanomaterials are easy to aggregate, which affects the distribution of enzymes on the electrode surface and the orientation of enzymes on the electrode surface, and further affects the overall performance of enzyme electrodes. On the other hand, the application of electrodeposition makes the distribution of film-forming materials on the electrode surface uneven, and the stability and repeatability of the electrode surface are poor. [0003] With the continuous development of nanotechnology, electrospinning technology has attracted more and more researchers' attention. Electrospun fibrous membranes have the a...

Claims

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Application Information

Patent Timeline
22 Aug 2012
Publication
CN102645474A
IPC
G01N27/327; H01M4/88; B82Y30/00; D01D5/00; D01D5/34; D01F8/02; D01F8/16; D01F1/10
CPC
Y02E60/50
Inventors
郑卫; 郭峰