Agarose aquogel fixed enzyme electrode and its preparation method and application

A technology of agarose hydrogel and immobilized enzymes, which is applied in the direction of material analysis, measuring devices, and instruments through electromagnetic means, which can solve the problems of unusable enzyme electrodes, poor biological affinity, and large mass transfer resistance, and achieve swelling Small effect, good biocompatibility, fast response effect

Inactive Publication Date: 2006-11-29
XIANGFAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantages are: the gels used in the traditional embedding method mainly include organic synthetic polymer materials such as polyacrylamide, polyvinyl chl...

Method used

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  • Agarose aquogel fixed enzyme electrode and its preparation method and application
  • Agarose aquogel fixed enzyme electrode and its preparation method and application
  • Agarose aquogel fixed enzyme electrode and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Preparation of hemoglobin-modified electrodes. Agarose (0.4g, agarose, Sigma) was dissolved in 100mL of boiling water, then cooled at room temperature to prepare a 0.4% hydrogel, and the mixed solution of agarose hydrogel and N, N-dimethylformamide (DMF) (agarose:DMF=4:1), and hemoglobin solution (1.34×10 -4 M) Equal volumes are mixed. Take 10 μl of the mixed solution and drop-coat it on the surface of the cracked graphite electrode, cover the small tube with holes, and dry it at room temperature for about 8 hours. The electrode has excellent performance, fast response time (30 seconds); long service life (30 days); high accuracy (<5%); good precision (<5‰) in aqueous phase, aqueous organic phase and organic phase Among them, it can detect nitric oxide, peroxide and ethyl chloride with a wide measurement range.

Embodiment 2

[0023] Prepare a myoglobin-modified electrode. Agarose (0.4g, agarose, Sigma) was dissolved in 100mL of boiling water, then cooled at room temperature to prepare a 0.4% hydrogel, and the mixed solution of agarose hydrogel and N, N-dimethylformamide (DMF) (agarose:DMF=4:1), and myoglobin solution (2.04×10 -5 M) Equal volumes are mixed. Take 10 μl of the mixed solution and drop-coat it on the surface of the cracked graphite electrode, cover the small tube with holes, and dry it at room temperature for about 8 hours. The electrode has excellent performance, fast response time (30 seconds); long service life (30 days); high accuracy (<5%); good precision (<5‰) in aqueous phase, aqueous organic phase and organic phase Among them, it can detect nitric oxide, peroxide and ethyl chloride with a wide measurement range.

Embodiment 3

[0025] Preparation of horseradish peroxidase modified electrode. Agarose (0.4g, agarose, Sigma) was dissolved in 100mL of boiling water, then cooled at room temperature to prepare a 0.4% hydrogel, and the mixed solution of agarose hydrogel and N, N-dimethylformamide (DMF) (agarose:DMF=4:1), and horseradish peroxidase solution (7.82×10 -5 M) Equal volumes are mixed. Take 10 μl of the mixed solution and drop-coat it on the surface of the cracked graphite electrode, cover the small tube with holes, and dry it at room temperature for about 8 hours. The electrode has excellent performance, fast response time (30 seconds); long service life (30 days); high accuracy (<5%); good precision (<5‰) in aqueous phase, aqueous organic phase and organic phase Among them, it can detect peroxide and has a wide measurement range.

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Abstract

The invention provides an agar sugar water gel fixed enzyme electrode and the preparing method and application thereof, dissolving agar sugar of 0.4-0.8g into 100 mL of boiling water and preparing agar sugar water gel, mixing the agar sugar water gel with organic solvent, then mixing with enzyme solution and preparing mixed solution, drop-smearing the mixed solution on the surface of electrode substrate, drying at room temperature and obtaining the agar sugar fixed enzyme electrode. And the invention uses characters and good bioaffinity of agar sugar to prepare the agar sugar fixed enzyme electrode, which has a wide use range, and can be used in water phase, water-containing organic phase and organic phase and has biological catalytic activity, and has quick response, good stability and reproducibility, long service life, high accuracy and precision, and wide measuring range.

Description

technical field [0001] The invention relates to an immobilized enzyme electrode and its preparation method and application, in particular to an agarose hydrogel immobilized enzyme electrode and its preparation method and application. Background technique [0002] Electrochemical enzyme sensors combine the high sensitivity of electrochemical methods with the high selectivity of enzymes, and their main application is to quantitatively detect the content of components in complex samples. In order to improve the specificity, sensitivity, accuracy, stability and service life of the electrochemical enzyme sensor, the research work mainly focuses on the optimization of the conditions for the construction of the enzyme sensor, such as the selection of immobilized enzyme materials, immobilization methods, appropriate media and Electrode base materials, etc. [0003] In the preparation of bioelectrochemical enzyme sensors, the most critical technologies include: (1) how to immobilize...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 刘慧宏
Owner XIANGFAN UNIVERSITY
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