Nano biologic enzyme electrode

A bio-enzyme and electrode technology, applied in the field of ultra-sensitive nano-bio-enzyme electrodes, can solve the problems of decreased effective active surface area, limited enzyme membrane sensitivity, and enzyme overlap

Inactive Publication Date: 2006-05-03
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that it fails to solve the problem of easy agglomeration between nanoparticles, and the agglomeration will cause the immobilized enzymes to overlap each other, and the effective active surface area will decrease, which limits the su

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Step (1) Dissolve 20g of ethylenediamine in 100mL of methanol, stir, blow nitrogen for 15min, add 1.36mol of methyl acrylate dropwise with a constant flow pump at -30°C, react at low temperature for 48h, then raise the temperature to room temperature (20°C) let it ripen for 48h. After the reaction was complete, the solvent methanol and excess methyl acrylate were distilled off under reduced pressure at a temperature lower than 45°C and 0.1 MPa to obtain 0.5G PAMAM.

[0037] Dissolve 25mmol of -0.5G PAMAM above in 100mL of methanol, dropwise add 200mmol of ethylenediamine in methanol solution with a constant flow pump, react at low temperature for 48h, and then react at room temperature for 48h. Also after the reaction is complete, distill under reduced pressure below 45°C and 0.1 MPa, add n-butanol and ethylenediamine to form an azeotropic mixture, and remove it to obtain 1G PAMAM.

[0038] By repeating the above synthesis process, half-generation or whole-generation P...

Embodiment 2

[0047] 0.1M K with a volume ratio of 3:1 2 PtCl 4 and 0.1M of K 2 PdCl 4 Mixed solution replaces the K of 0.1M in embodiment 1 step (2) 2 PtCl 4 Aqueous solution, repeating each step in embodiment 1, finally obtains the platinum electrode that the surface contains the bimetallic Pt / Pd nanoparticle of dendrimer encapsulation and the multilayer sensing film that glucose oxidase forms, and is used for the glucose oxidase sensor detection. Under the same process conditions, when the glucose concentration in the bottom solution is 1mM, the sensitivity of the electrode is 720μA / mM·cm 2 , which is 1200 times more sensitive than the unenhanced blank enzyme electrode.

Embodiment 3

[0049] Replace the glucose oxidase in the step (4) of Example 1 with catalase, replace the glucose in the bottom solution with hydrogen peroxide, repeat the steps in Example 1, and finally obtain the single compound containing dendrimer encapsulation on the surface. A platinum electrode with a multilayer sensing film formed of metal Pt nanoparticles and catalase, and used for the detection of hydrogen peroxide. Under the same process conditions, when the concentration of hydrogen peroxide in the bottom solution is 1mM, the sensitivity of the electrode is 480μA / mM cm 2 , compared with the sensitivity of the blank enzyme electrode without enhancement (1.1μA / mM·cm 2 ) increased by 430 times.

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Abstract

The invention relates to a nanometer biology enzyme electrode which is formed by the tree-sharp macromolecular packaging mental nanometer and enzyme self-assembling. The nanometer particle is packaged inside the tree-sharp macromolecular to keep better activity; the electrode surface forms multi-foil layer with better scattering character and nanometer mental particle which can abridge the respond time and improve the sensing ability of the enzyme sensor. It can make super sensitive nanometer biology enzyme electrode by choosing different enzyme oxide needed by different applied.

Description

technical field [0001] The invention relates to a biochemical electrode used for analyzing materials by testing electrochemical variables, in particular to a supersensitive nano-biological enzyme electrode reinforced with nano-metal particles, and its preparation method. Background technique [0002] Biosensors are widely used in clinical diagnosis, industrial control, food and drug analysis, biopharmaceutical development, environmental analysis, military field, biotechnology and biochip due to their advantages of small size, high precision, good sensitivity and convenient on-site determination. have important uses. For example, a glucose biosensor assembled using glucose oxidase can be used to measure human blood sugar, industrial wastewater, sugar in fermentation or beverages. However, the bioenzyme electrodes currently available in the market for biosensors such as glucose and cholesterol have many disadvantages, such as large background current, poor response characteri...

Claims

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Application Information

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IPC IPC(8): G01N27/327C12Q1/00
Inventor 朱以华杨晓玲朱辉钰郭飞
Owner EAST CHINA UNIV OF SCI & TECH
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