Automatic reaction device and method for pretreating cells

An automatic reaction and pretreatment technology, applied in the field of cell analysis, can solve the problems of bulky, complex and bulky cell pretreatment system, and achieve the effects of reducing reaction volume, improving pretreatment efficiency, safe and accurate nucleic acid detection

Active Publication Date: 2015-06-03
XI AN JIAOTONG UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide an automatic reaction device and method for pretreatment of cells to solve the complex, bulky and cumbersome problems of the existing cell pretreatment system; Crushing and lysis, purification and extraction of nucleic acid, and PCR amplification are integrated in one reaction box

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  • Automatic reaction device and method for pretreating cells
  • Automatic reaction device and method for pretreating cells

Examples

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example 1

[0031] Example 1: The blood cell pretreatment method based on the automatic reaction device, comprising the following steps:

[0032] Step 1, cell lysis: inject 600 μL of diluent into the magnetic bead chamber 8, then inhale into the middle chamber 7, and repeatedly push and pull the piston 5 to dissolve, after the magnetic beads are attracted by the magnet at the bottom of the middle chamber 7, push the diluent back to the magnetic bead cavity 8, then inject 500 μL of lysate, 200 μL of blood sample and 20 μL of proteinase K into the sample cavity 12 to be tested, then inhale into the middle cavity 7, release the magnetic beads and repeatedly push and pull the piston 5 to carry out the mixing reaction, and then the magnetic beads Adsorb to the bottom, and push the reaction solution back to the sample cavity 12 to be tested;

[0033] Step 2, nucleic acid purification: Inject 700 μL of the first washing liquid into the first washing liquid chamber 9, suck it into the middle cham...

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Abstract

The invention discloses automatic reaction device and method for pretreating cells. The device comprises a reaction box body, a base, a box cover and a piston, wherein a middle cavity is formed in the center of the reaction box body; seven cavities are formed in the periphery of the hollow cavity; seven square cavities are correspondingly formed between the middle cavity and the seven cavities in the periphery; a control valve is put into each square cavity; communication between an inner chamber and an outer chamber is controlled through up and down movement; the lower part of the reaction box body is tightly connected with the base; the box cover is arranged at the upper part of the reaction box body; a piston rod which is matched with the middle cavity penetrates through the box cover; and the outer side of a second PCR reagent cavity is connected with a PCR chip for detecting nucleic acid. According to the reaction device and method, crushing and cracking of original samples, and purifying and extracting of the nucleic acid and PCR amplification are integrated in a reaction box; the operation process is simplified; the pretreatment efficiency is greatly improved; meanwhile, the reaction volume is reduced; the portability of an instrument is improved; and a foundation is laid for rapid, safe and accurate nucleic acid detection.

Description

【Technical field】 [0001] The invention relates to the technical field of cell analysis, in particular to an automatic reaction device and method for pretreating cells. 【Background technique】 [0002] Nucleic acid detection is the most eye-catching field in the post-genome era, and it will have an immeasurable impact on genetics, clinical medicine, and molecular diagnostics. In order to complete high-quality nucleic acid detection, cells need to be pretreated quickly and efficiently to extract high-purity nucleic acids. Cell pretreatment mainly includes three steps of cell lysis, nucleic acid purification and PCR amplification, aiming to purify and proliferate the nucleic acid in the cells after pulverizing the cells to achieve the purity and concentration required for detection. [0003] At present, the pretreatment of cells on the market generally adopts an extension operation, that is, each step of the operation is completed on multiple instruments, which will not only ma...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N15/10
Inventor 韦学勇南浪李政苗保刚蒋庄德彭年才
Owner XI AN JIAOTONG UNIV
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